Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after ampl...

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Principais autores: Maciel, Bruna Leal Lima, Medeiros, Amanda Fernandes de, Costa, Izael de Sousa, Carvalho, Fabiana Maria Coimbra de, Kiyota, Sumika, Souza, Beatriz Blenda Pinheiro de, Sifuentes, Daniel Nogoceke, Serquiz, Raphael Paschoal, Uchôa, Adriana Ferreira, Santos, Elizeu Antunes dos, Morais, Ana Heloneida de Araújo
Formato: article
Idioma:English
Publicado em: Journal of Enzyme Inhibition and Medicinal Chemistry
Assuntos:
Tamarind
Antitryptic
Wistar rats
CCK
Endereço do item:https://repositorio.ufrn.br/handle/123456789/57470
http://dx.doi.org/10.1080/14756366.2017.1419220
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spelling ri-123456789-574702024-02-01T16:00:59Z Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity Maciel, Bruna Leal Lima Medeiros, Amanda Fernandes de Costa, Izael de Sousa Carvalho, Fabiana Maria Coimbra de Kiyota, Sumika Souza, Beatriz Blenda Pinheiro de Sifuentes, Daniel Nogoceke Serquiz, Raphael Paschoal Uchôa, Adriana Ferreira Santos, Elizeu Antunes dos Morais, Ana Heloneida de Araújo Tamarind Antitryptic Wistar rats CCK A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M þ 14H]þ ¼ 19,594,690 Da and [M þ 13H]þ ¼ 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 1010mol.L1 and Ki was 2.9 1011mol.L1 . The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application 2024-02-01T16:00:58Z 2024-02-01T16:00:58Z 2018-01 article MEDEIROS, Amanda Fernandes de; COSTA, Izael de Sousa; CARVALHO, Fabiana Maria Coimbra de; KIYOTA, Sumika; SOUZA, Beatriz Blenda Pinheiro de; SIFUENTES, Daniel Nogoceke; SERQUIZ, Raphael Paschoal; MACIEL, Bruna Leal Lima; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos; MORAIS, Ana Heloneida de Araújo. Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity. Journal Of Enzyme Inhibition And Medicinal Chemistry, [S.l.], v. 33, n. 1, p. 334-348, 1 jan. 2018. DOI: 10.1080/14756366.2017.1419220. Disponível em: https://www.tandfonline.com/doi/full/10.1080/14756366.2017.1419220. Acesso em: 30 jan. 2024. https://repositorio.ufrn.br/handle/123456789/57470 http://dx.doi.org/10.1080/14756366.2017.1419220 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Journal of Enzyme Inhibition and Medicinal Chemistry
institution Repositório Institucional
collection RI - UFRN
language English
topic Tamarind
Antitryptic
Wistar rats
CCK
spellingShingle Tamarind
Antitryptic
Wistar rats
CCK
Maciel, Bruna Leal Lima
Medeiros, Amanda Fernandes de
Costa, Izael de Sousa
Carvalho, Fabiana Maria Coimbra de
Kiyota, Sumika
Souza, Beatriz Blenda Pinheiro de
Sifuentes, Daniel Nogoceke
Serquiz, Raphael Paschoal
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
Morais, Ana Heloneida de Araújo
Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
description A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M þ 14H]þ ¼ 19,594,690 Da and [M þ 13H]þ ¼ 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 1010mol.L1 and Ki was 2.9 1011mol.L1 . The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application
format article
author Maciel, Bruna Leal Lima
Medeiros, Amanda Fernandes de
Costa, Izael de Sousa
Carvalho, Fabiana Maria Coimbra de
Kiyota, Sumika
Souza, Beatriz Blenda Pinheiro de
Sifuentes, Daniel Nogoceke
Serquiz, Raphael Paschoal
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
Morais, Ana Heloneida de Araújo
author_facet Maciel, Bruna Leal Lima
Medeiros, Amanda Fernandes de
Costa, Izael de Sousa
Carvalho, Fabiana Maria Coimbra de
Kiyota, Sumika
Souza, Beatriz Blenda Pinheiro de
Sifuentes, Daniel Nogoceke
Serquiz, Raphael Paschoal
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
Morais, Ana Heloneida de Araújo
author_sort Maciel, Bruna Leal Lima
title Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_short Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_full Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_fullStr Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_full_unstemmed Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
title_sort biochemical characterisation of a kunitz-type inhibitor from tamarindus indica l. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity
publisher Journal of Enzyme Inhibition and Medicinal Chemistry
publishDate 2024
url https://repositorio.ufrn.br/handle/123456789/57470
http://dx.doi.org/10.1080/14756366.2017.1419220
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