Atividade antioxidante de fucoidan modificado pelo método redox com ácido gálico
Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capac...
Uloženo v:
| Hlavní autor: | |
|---|---|
| Další autoři: | |
| Médium: | doctoralThesis |
| Jazyk: | pt_BR |
| Vydáno: |
Universidade Federal do Rio Grande do Norte
|
| Témata: | |
| On-line přístup: | https://repositorio.ufrn.br/handle/123456789/50945 |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!
|
| Shrnutí: | Antioxidant compounds decrease the amount of intracellular reactive oxygen species
(ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts.
Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox
method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells
(MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 ± 3.0 mg GA per
gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total
antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three
times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to
H2O2 increased ROS levels and activated caspase-3 along with caspase-9. In
addition, the cell viability decreased approximately 80%. FucA-GA also provided the
most effective protection against oxidative damage caused by H2O2. Treatment with
FucA-GA (1.0 mg/mL) increased cell viability (~80%) and decreased intracellular
ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg/mL)
abolished H2O2-induced oxidative stress in zebra fish embryos. Overall, FucA-GA
protected MC3T3 cells from oxidative stress and could represent a possible adjuvant
for the treatment of bone fragility by counteracting oxidative phenomena. |
|---|