Identificação de aflatoxina b1 em amostras de farelo de amendoim vendidas como complemento para o açaí.
Introduction: One of the problems related to peanut production is aflatoxin, a mycotoxin from secondary metabolites produced by fungi, belonging to the genus Aspergillus sp. The aflatoxins AFB1, AFB2, AFG1 e AFG2 are commonly found in peanut samples, among these, AFB1 the most common to be found...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/35696 |
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| Resumo: | Introduction: One of the problems related to peanut production is aflatoxin, a mycotoxin from
secondary metabolites produced by fungi, belonging to the genus Aspergillus sp. The aflatoxins
AFB1, AFB2, AFG1 e AFG2 are commonly found in peanut samples, among these, AFB1 the
most common to be found in food and classified as the most toxic. Objective: To investigate
the presence of Aflatoxin B1 in peanut flour samples sold as an accompaniment to the açaí
consumed and commercially available in Natal/RN. Methodology: Twelve samples of peanut
bran were analyzed, based on cost and location of points. The collections were made in two
different years. The samples were from low-cost, high-cost açaí and supermarket açaí in the
city. The extraction and purification method used in this research was adapted from the
methodology established by the International Official Methods of Analysis (AOAC). In the
detection of aflatoxins, the analysis was done under ultraviolet light and it was used a ThinLayer Chromatography (TLC) of silica with fluorescence. The samples which reported
flowering in the TLC were confirmed with chemical affirmation, spraying the 25% aqueous
sulfuric acid solution on the chromatographic plates. Results: Two samples presented aflatoxin
B1 contamination and they were confirmed using the chemical affirmer. Conclusion:
Confirmed the need to monitor and control these products consumed worldwide and the
application of more sensitive analytical methods to identify and quantify by comparison with
the current legislation and the need for larger sampling. |
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