Estudo da formação de agregados de agarose através de análise reológica e espalhamento dinâmico de luz
The agarose is a natural polymer extract from red algae (Rhodophyceae). This polysaccharide can form physical gels when is removed heat from the system. This property has great applicability in the food and pharmaceutical industries, particularly in DNA analysis. For this work we prepared aqueous so...
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| Formato: | Dissertação |
| Idioma: | por |
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| Endereço do item: | https://repositorio.ufrn.br/jspui/handle/123456789/22377 |
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| Resumo: | The agarose is a natural polymer extract from red algae (Rhodophyceae). This polysaccharide can form physical gels when is removed heat from the system. This property has great applicability in the food and pharmaceutical industries, particularly in DNA analysis. For this work we prepared aqueous solutions at different concentrations of agarose. Measures of Dynamic Light Scattering (DLS) were made while the system temperature was altered so as to obtain structures more or less organized of macromolecules. For DLS measurements, the Kohrausch-William-Watts equation (KWW) was fitted to the data to obtain the average of relaxation rate of the process. Additionally rheometry tests were made also with the temperature variation. For both techniques, were made mathematical adjustments with the intention of studying the helix-coil transition of the polymer chains during the temperature change, relating them by calculating the apparent activation energy. In both approaches it was found that energy tends to decrease for dilute solutions and higher temperatures. |
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