A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells

Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several...

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Principais autores: Morais, Ana Heloneida de Araújo, Rabelo, Luciana, Monteiro, Norberto de Kássio Vieira, Serquiz, Raphael Paschoal, Santos, Paula, Oliveira, Ruth, Oliveira, Adeliana Silva de, Rocha, Hugo, Uchôa, Adriana Ferreira, Santos, Elizeu Antunes dos
Formato: article
Idioma:English
Publicado em: Marine Drugs
Assuntos:
HeLa
Lectin
Antitumor
Marine sponge
Cinachyrella apion
Endereço do item:https://repositorio.ufrn.br/handle/123456789/58138
http://dx.doi.org/10.3390/md10040727
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spelling ri-123456789-581382024-04-12T21:25:02Z A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells Morais, Ana Heloneida de Araújo Rabelo, Luciana Monteiro, Norberto de Kássio Vieira Serquiz, Raphael Paschoal Santos, Paula Oliveira, Ruth Oliveira, Adeliana Silva de Rocha, Hugo Uchôa, Adriana Ferreira Santos, Elizeu Antunes dos HeLa Lectin Antitumor Marine sponge Cinachyrella apion Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer 2024-04-12T21:25:01Z 2024-04-12T21:25:01Z 2012-03 article RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024. https://repositorio.ufrn.br/handle/123456789/58138 http://dx.doi.org/10.3390/md10040727 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Marine Drugs
institution Repositório Institucional
collection RI - UFRN
language English
topic HeLa
Lectin
Antitumor
Marine sponge
Cinachyrella apion
spellingShingle HeLa
Lectin
Antitumor
Marine sponge
Cinachyrella apion
Morais, Ana Heloneida de Araújo
Rabelo, Luciana
Monteiro, Norberto de Kássio Vieira
Serquiz, Raphael Paschoal
Santos, Paula
Oliveira, Ruth
Oliveira, Adeliana Silva de
Rocha, Hugo
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
description Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer
format article
author Morais, Ana Heloneida de Araújo
Rabelo, Luciana
Monteiro, Norberto de Kássio Vieira
Serquiz, Raphael Paschoal
Santos, Paula
Oliveira, Ruth
Oliveira, Adeliana Silva de
Rocha, Hugo
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
author_facet Morais, Ana Heloneida de Araújo
Rabelo, Luciana
Monteiro, Norberto de Kássio Vieira
Serquiz, Raphael Paschoal
Santos, Paula
Oliveira, Ruth
Oliveira, Adeliana Silva de
Rocha, Hugo
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
author_sort Morais, Ana Heloneida de Araújo
title A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_short A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_full A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_fullStr A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_full_unstemmed A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_sort lactose-binding lectin from the marine sponge cinachyrella apion (cal) induces cell death in uuman cervical adenocarcinoma cells
publisher Marine Drugs
publishDate 2024
url https://repositorio.ufrn.br/handle/123456789/58138
http://dx.doi.org/10.3390/md10040727
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