Competência vetorial de Triatoma brasiliensis e Triatoma pseudomaculata (Hemiptera: Reduviidae) infectados com diferentes DTUs (Discrete Typing Units) de Trypanosoma cruzi
The objective of this study was to evaluate the biological aspects of Triatoma brasiliensis and Triatoma pseudomaculata experimentally infected by different DTUs (Discrete Typing Units) of Trypanosoma cruzi. Nymphs of the 3rd, 4th and 5th instars of T. brasiliensis (n=120) and T. pseudomaculata (...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/57174 |
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| Resumo: | The objective of this study was to evaluate the biological aspects of Triatoma brasiliensis and
Triatoma pseudomaculata experimentally infected by different DTUs (Discrete Typing Units)
of Trypanosoma cruzi. Nymphs of the 3rd, 4th and 5th instars of T. brasiliensis (n=120) and T.
pseudomaculata (n=120) were fed to mice infected with a T. cruzi isolate or a mixture of
parasite isolates. An uninfected group of insects was used as a control. Triatomine infection by
T. cruzi was assessed daily and individually by analyzing excreta up to 90 days after infection.
The biological aspects of triatomines, such as excretion, occurrence of ecdysis and mortality,
were evaluated daily. The volume of blood ingested (μL) and weight (mg) 4 hours after the
insects' blood meal were checked every 15 or 30 days. Infection by T. cruzi was detected in all
insects of the species T. brasiliensis, while in T. pseudomaculata it was observed only in
specimens with a single infection with isolate RN02 and in those infected with isolates
RN02+RN23 and RN02+RN23+ CL - Brener clone. The average percentage of infection of the
species T. brasiliensis was 23.8% and 31.4%, in insects that received a blood meal at intervals
of 15 and 30 days, respectively. For T. pseudomaculata, the average percentage of infection
was 28.6% (feeding every 15 days) and 15.4% (feeding every 30 days). The volume of blood
ingested by insects T. brasiliensis and T. pseudomaculata with mixed infection was lower than
that of insects with single infection and the control. The occurrence of ecdysis was higher in
the T. brasiliensis species with single infection and in T. pseudomaculata control insects.
Mortality was higher in T. pseudomaculata with mixed infection, while no significant
difference was observed in the T. brasiliensis species. Excretion was higher in T.
pseudomaculata control insects. For the first time we observed the occurrence of
kleptohematophagy in T. brasiliensis. These data showed that these two species are potentially
effective vectors in the transmission of T. cruzi, with T. brasiliensis being more susceptible to
infection. Therefore, it is essential to carry out vector control activities continuously and
efficiently to reduce the risk of T. cruzi transmission in endemic areas where these vectors occur. |
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