Avaliação de alimentador artificial para Lutzomyia longipalpis e melhorias na utilização de baterias da armadilha CDC
Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is the main vector of the protozoan Leishmania infantum in Brazil. Its surveillance is carried out by surveillance centers, such as the Zoonosis Surveillance Unit (UVZ) in Natal-RN. In the field, this vector is monitored throug...
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Formato: | Dissertação |
Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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Endereço do item: | https://repositorio.ufrn.br/handle/123456789/55660 |
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Resumo: | Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is the main vector
of the protozoan Leishmania infantum in Brazil. Its surveillance is carried out by
surveillance centers, such as the Zoonosis Surveillance Unit (UVZ) in Natal-RN. In the
field, this vector is monitored through collections using the CDC trap. In addition,
laboratory maintenance requires blood ingestion, usually from a vertebrate, which can
bring contamination risks to the colony and impair the food source. This study aimed
to standardize an artificial feeding model for L. longipalpis in a laboratory environment
and enable the use of solar energy for the functioning of CDC traps. Feeders commonly
used in mosquito colonies were tested. An artificial feeder called Flebfeeding was also
constructed, using equipment such as a thermostat, boiler, aquarium pump, plastic
hoses, water heating reservoir, and a flat-bottomed flask. The results showed that the
FlebFeeding system was efficient in maintaining the blood temperature during feeding
and attracting insects, with an average feeding success rate of 53% for females.
Engorged females laid an average of 17.49 eggs, and the eggs were viable, with the
hatching of larvae and adults in the rearing pots. To evaluate the efficiency of solar
charging, a charger was built with a 100W solar panel directly connected to wires
attached to the positive and negative terminals of a voltage regulator and the battery.
Although the charging took longer than the conventional charger, about 20 hours to
complete, with an average current of 0.44A, it was found that the charging was efficient
and had a similar field use durability to a conventionally charged battery, which took
an average of 10.6 hours to reach full battery charge, with an average current of 1A.
The results indicate that artificial feeding through Flebfeeding is effective for L.
longipalpis females and offers advantages over conventional feeding, as reducing the
use of animals in laboratory experiments is an important ethical principle. As for
charging CDC trap batteries with photovoltaic panels, it is a viable option and may
potentially generate savings in public expenses. However, further research and costbenefit analyses are necessary to evaluate the feasibility of the techniques on a larger scale. |
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