Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos
Species of the genus Candida are responsible for about 80% of serious fungal infections, being Candida albicans the most commonly isolated species in these cases, and it is also frequently associated with cutaneous and mucocutaneous fungal infections. Among the filamentous fungi responsible for c...
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Candidíases Dermatofitoses Produtos naturais Fitoconstituintes CNPQ::CIENCIAS BIOLOGICAS |
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Candidíases Dermatofitoses Produtos naturais Fitoconstituintes CNPQ::CIENCIAS BIOLOGICAS Sales, Fábio do Nascimento Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos |
| description |
Species of the genus Candida are responsible for about 80% of serious fungal infections,
being Candida albicans the most commonly isolated species in these cases, and it is also
frequently associated with cutaneous and mucocutaneous fungal infections. Among the
filamentous fungi responsible for cutaneous mycoses in humans, dermatophytes, a group of
crosely related fungi, affect 25% of the world population. Furthermore, both Candida and
dermatophyte species have shown resistance to antifungal drugs. In this context, it is
perceived an incessant search of the scientific community for molecular prototypes that
present antifungal potential, such as those of plant origin, due to their many antimicrobial
properties described in the literature. Therefore, the present proposal aims to evaluate the
antifungal activity of the essential oil from the plant Lippia grata Schauer (Verbenaceae)
against Candida albicans and different species of dermatophyte fungi. The essential oil was
obtained from the hydrodistillation process, with subsequent analysis of percentage content
(%) and phytochemistry by gas chromatography associated with mass spectrometry. The
bioactivity of the essential oil obtained from the plant Lippia grata was investigated on 26
fungal samples by the method of Minimum Inhibitory Concentration (MIC) by the
microdilution broth technique and Minimum Fungicidal Concentration (MFC) using solid
medium, while the synergistic activity was evaluated using the “checkerboard” method. The
quantification tests of biofilm production and antibiofilm activity of Lippia grata essential oil
were performed using crystal violet to measure the optical densities obtained. The yield of
essential oil of Lippia grata was 2.45%. Four main phytoconstituents were identified in the
chemical characterization of Lippia grata essential oil: carvacrol (51.4%) as the majority
constituent, thymol (15.6%), ρ-cymene (12.2%) and γ-terpinene (7.4%). Antifungal activity of
Lippia grata essential oil was found for 100% of the tested strains with MIC and MFC
ranging from 390.62 µg/mL to 3.125 µg/mL against Candida albicans strains. For
dermatophytes, the MIC ranged from 48.82 µg/mL to 390.62 µg/mL, while the MFC ranged
from 48.82 µg/mL a 781.25 µg/mL. Regarding the evaluation of the effect of combinations of
drugs, synergistic (50%) and additive (50%) interactions were observed on the strains tested.
The results of biofilm formation revealed that 100% of the strains were biofilm producers. For
Candida albicans strains, 4 (22.22%) were weak producing strains, 7 (38.88%) moderate
producing strains and 7 (38.88%) strong producing strains. For dermatophyte strains, 2 (25%)
moderate producing strains and 6 (75%) were strong producing strains. For the antibiofilm
activity of the essential oil, it was verified inhibition of biofilm ranging from 28.8.% to 72.4%
for Candida albicans strains and 4.39% to 90.4% for dermatophytes, when these strains were
post-treated, respectively, with concentrations of 2xCIM, CIM e 2/CIM of Lippia grata
essential oil. It was concluded that the essential oil of Lippia grata showed antifungal activity,
motivating new studies with the intention of future development of phytodrugs directed to the
treatment of fungal infections. |
| author2 |
Andrade, Vânia Sousa |
| author_facet |
Andrade, Vânia Sousa Sales, Fábio do Nascimento |
| format |
masterThesis |
| author |
Sales, Fábio do Nascimento |
| author_sort |
Sales, Fábio do Nascimento |
| title |
Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos |
| title_short |
Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos |
| title_full |
Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos |
| title_fullStr |
Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos |
| title_full_unstemmed |
Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos |
| title_sort |
atividade antifúngica do óleo essencial de lippia grata schauer (verbenaceae) frente candida albicans e dermatófitos |
| publisher |
Universidade Federal do Rio Grande do Norte |
| publishDate |
2023 |
| url |
https://repositorio.ufrn.br/handle/123456789/55256 |
| work_keys_str_mv |
AT salesfabiodonascimento atividadeantifungicadooleoessencialdelippiagrataschauerverbenaceaefrentecandidaalbicansedermatofitos |
| _version_ |
1797696652151095296 |
| spelling |
ri-123456789-552562024-03-19T04:04:46Z Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos Sales, Fábio do Nascimento Andrade, Vânia Sousa http://lattes.cnpq.br/7461951524172543 https://orcid.org/0000-0003-4138-4373 http://lattes.cnpq.br/9327124853897215 Bastos, Rafael Wesley https://orcid.org/0000-0001-6781-9617 http://lattes.cnpq.br/3333946080391361 Ximenes, Eulália Camelo Pessoa de Azevedo Candidíases Dermatofitoses Produtos naturais Fitoconstituintes CNPQ::CIENCIAS BIOLOGICAS Species of the genus Candida are responsible for about 80% of serious fungal infections, being Candida albicans the most commonly isolated species in these cases, and it is also frequently associated with cutaneous and mucocutaneous fungal infections. Among the filamentous fungi responsible for cutaneous mycoses in humans, dermatophytes, a group of crosely related fungi, affect 25% of the world population. Furthermore, both Candida and dermatophyte species have shown resistance to antifungal drugs. In this context, it is perceived an incessant search of the scientific community for molecular prototypes that present antifungal potential, such as those of plant origin, due to their many antimicrobial properties described in the literature. Therefore, the present proposal aims to evaluate the antifungal activity of the essential oil from the plant Lippia grata Schauer (Verbenaceae) against Candida albicans and different species of dermatophyte fungi. The essential oil was obtained from the hydrodistillation process, with subsequent analysis of percentage content (%) and phytochemistry by gas chromatography associated with mass spectrometry. The bioactivity of the essential oil obtained from the plant Lippia grata was investigated on 26 fungal samples by the method of Minimum Inhibitory Concentration (MIC) by the microdilution broth technique and Minimum Fungicidal Concentration (MFC) using solid medium, while the synergistic activity was evaluated using the “checkerboard” method. The quantification tests of biofilm production and antibiofilm activity of Lippia grata essential oil were performed using crystal violet to measure the optical densities obtained. The yield of essential oil of Lippia grata was 2.45%. Four main phytoconstituents were identified in the chemical characterization of Lippia grata essential oil: carvacrol (51.4%) as the majority constituent, thymol (15.6%), ρ-cymene (12.2%) and γ-terpinene (7.4%). Antifungal activity of Lippia grata essential oil was found for 100% of the tested strains with MIC and MFC ranging from 390.62 µg/mL to 3.125 µg/mL against Candida albicans strains. For dermatophytes, the MIC ranged from 48.82 µg/mL to 390.62 µg/mL, while the MFC ranged from 48.82 µg/mL a 781.25 µg/mL. Regarding the evaluation of the effect of combinations of drugs, synergistic (50%) and additive (50%) interactions were observed on the strains tested. The results of biofilm formation revealed that 100% of the strains were biofilm producers. For Candida albicans strains, 4 (22.22%) were weak producing strains, 7 (38.88%) moderate producing strains and 7 (38.88%) strong producing strains. For dermatophyte strains, 2 (25%) moderate producing strains and 6 (75%) were strong producing strains. For the antibiofilm activity of the essential oil, it was verified inhibition of biofilm ranging from 28.8.% to 72.4% for Candida albicans strains and 4.39% to 90.4% for dermatophytes, when these strains were post-treated, respectively, with concentrations of 2xCIM, CIM e 2/CIM of Lippia grata essential oil. It was concluded that the essential oil of Lippia grata showed antifungal activity, motivating new studies with the intention of future development of phytodrugs directed to the treatment of fungal infections. Espécies do gênero Candida são responsáveis por cerca de 80% das infecções fúngicas graves, sendo a espécie Candida albicans a mais comumente isolada nestes casos, além de ser associada frequentemente a infecções fúngicas cutâneas e mucocutâneas. Dentre os fungos filamentosos responsáveis por micoses cutâneas em humanos, os dermatófitos, um grupo de fungos estritamente relacionados, afetam 25% da população mundial e entre 18 a 23% da população brasileira. Além disso, tanto às espécies de Candida quanto de dermatófitos passaram a apresentar resistência aos antifúngicos nas últimas décadas. Diante desse contexto, percebe-se uma busca incessante da comunidade científica por protótipos moleculares que apresentem potencial antifúngico, como os de origem vegetal, devido a suas muitas propriedades antimicrobianas descritas na literatura. Portanto, este trabalho teve como objetivo avaliar a atividade antifúngica do óleo essencial da planta Lippia grata Schauer (Verbenaceae) frente leveduras do gênero Candida e diferentes espécies de fungos dermatófitos. O óleo essencial foi obtido a partir do processo de hidrodestilação, com posterior análise de seu teor percentual (%) e fitoquímica realizada por cromatografia gasosa associada à espectofotometria de massas. A bioatividade do óleo essencial obtido da planta Lippia grata foi investigada sobre 26 amostras fúngicas pelo método de Concentração Inibitória Mínima (CIM) pela técnica de microdiluição em caldo e Concentração Fungicida Mínima (CFM) utilizando meio sólido, enquanto a atividade sinérgica foi avaliada utilizando o método “checkerboard”. Os testes de quantificação da produção de biofilme e atividade antibiofilme do óleo essencial de Lippia grata foram realizados utilizando o cristal violeta para mensurar as densidades ópticas obtidas. O rendimento do óleo essencial de Lippia grata foi de 2.45%. Foram identificados quatro principais fitoconstituintes na caracterização química do óleo essencial de Lippia grata: carvacrol (51.4%) como constituinte majoritário, timol (15.6%), ρ-cimeno (12.2%) e γ-terpineno (7.4%). Constatou-se atividade antifúngica do óleo essencial de Lippia grata para 100% das cepas testadas com CIM e CFM variando entre 390.62 µg/mL a 3.125 µg/mL para cepas de Candida albicans. Para dermatófitos, a CIM variou entre 48.82 µg/mL a 390.62 µg/mL, enquanto a CFM variou de 48.82 µg/mL a 781.25 µg/mL. Com relação à avaliação do efeito de combinações de drogas, observou-se interação sinérgica (50%) e aditiva (50%) sobre as cepas testadas. Os resultados para a formação de biofilme revelaram que 100% das cepas foram produtoras de biofilme. Para as cepas de Candida albicans, 4 (22.22%) foram fracas produtoras, 7 (38.88%) moderadas produtoras e 7 (38.88%) fortes produtoras. Para as cepas de dermatófitos, 2 (25%) moderadas produtoras e 6 (75%) foram fortes produtoras. Para a atividade antibiofilme do óleo essencial, verificou-se inibição de biofilme variando de 28.8% a 72.4% para as cepas de Candida albicans e 4.39% a 90.4% para dermatófitos, quando essas cepas foram pós-tratadas, respectivamente, com as concentrações de 2xCIM, CIM e 2/CIM do óleo essencial de Lippia grata. Concluiu-se que o óleo essencial de Lippia grata apresentou atividade antifúngica, motivando novos estudos com o intuito de desenvolvimento futuro de fitofármacos direcionados ao tratamento de infecções fúngicas. 2024-06-29 2023-11-09T23:42:39Z 2023-03-24 masterThesis SALES, Fábio do Nascimento. Atividade antifúngica do óleo essencial de Lippia grata Schauer (Verbenaceae) frente Candida albicans e dermatófitos. Orientadora: Dra. Vânia Sousa Andrade. 2023. 132f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2023. https://repositorio.ufrn.br/handle/123456789/55256 pt_BR Acesso Embargado application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM BIOLOGIA PARASITÁRIA |