Potencial biotecnológico de Synechococcus nidulans cultivada em meio alternativo utilizando extrato de bagaço de caju
The cultivation of microalgae has attracted a lot of attention in recent years, since these microorganisms are sources of bioactive compounds that offer a wide area of application, including in the area of cosmetics. Particularly, Brazil has the fourth largest cosmetics market in the world, as we...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Acceso en liña: | https://repositorio.ufrn.br/handle/123456789/55195 |
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| Resumo: | The cultivation of microalgae has attracted a lot of attention in recent years, since these
microorganisms are sources of bioactive compounds that offer a wide area of application,
including in the area of cosmetics. Particularly, Brazil has the fourth largest cosmetics
market in the world, as well as favorable climatic conditions for the cultivation of
microalgae. However, given the numerous species of microalgae that are little explored
and the high cost of biomass production, it is necessary to invest in research that involves
the study of little-studied species and low-cost biomass production strategies. In this
sense, the objective of this research was to evaluate the potential of the microalgae
Synechococcus nidulans, a species little studied, using mixotrophic cultures with the
addition of Cashew Bagasse Extract (EBC) as an organic carbon source in order to
increase biomass production and evaluate the potential production of bioactive
compounds of interest to the cosmetics industry. S. nidulans cultivation experiments were
carried out under autotrophic and mixotrophic conditions with different concentrations of
EBC (0.5%, 1.5% and 2.5% (v/v)) and the biomass composition was determined in terms
of carbohydrates, proteins, lipids, ash and carotenoids. Cultivations were carried out in
triplicate, under controlled conditions (temperature of 24 ± 1ºC, light intensity of
approximately 310 µmol/m2/s photons, provided by 5 fluorescent lamps of 40 W,
constant aeration, photoperiod of 12 h light/dark). Crops subjected to stress with EBC
indicated better biomass yields. A 25.9% drop in ash levels was observed in mixotrophic
cultivation with the addition of 2.5% (v/v) of EBC, unlike carbohydrate levels, which
increased by up to 28.5% with the addition of 2.5% (v/v) of EBC in relation to the control
culture. The results showed that the use of EBC in the production of bioactives by
microalgae reduced the yields of total lipids and total carotenoids in relation to the control
culture (13.22% ±0.25% and 10.02mg/g ±4.33 mg/ g). However, the increase in biomass
production in mixotrophic crops justifies the increased production of bioactives at a lower
cost compared to control. Regarding total protein production, the cultivation with addition
of 2.5% EBC stood out, presenting 54.07% ±3.59%. Thus, the results demonstrate that
the microalgae under study contains biocompounds of interest to the cosmetics industry
and that these can be explored in mixotrophic cultivation with agro-industrial residues as
a source of organic carbon. |
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