Estrutura e diversidade do locus rfb em bactérias do gênero Leptospira e sua associação com a classificação sorológica
Leptospirosis is considered a globally important zoonosis due to its widespread distribution and virulence, affecting both humans and commercially important animals. It is caused by pathogenic bacteria of the genus Leptospira and phylum Spirochaetes, and contamination occurs through direct or ind...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/52699 |
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| Resumo: | Leptospirosis is considered a globally important zoonosis due to its widespread distribution
and virulence, affecting both humans and commercially important animals. It is caused by
pathogenic bacteria of the genus Leptospira and phylum Spirochaetes, and contamination
occurs through direct or indirect contact with the contaminant agent present in the
environment, such as urine from infected animals or contaminated water and soil. The genus
has 68 species that can be grouped into two major groups according to their lifestyle:
pathogenic and saprophytic. In addition to taxonomic classification, samples of these genera
can be classified based on their antigenic characteristics into serogroups and serovars.
Serological classification is of great relevance in the fields of epidemiology and clinical
analysis, but the methods used for this classification are laborious, require infrastructure and
specialized labor, and take days to obtain results. In this study, we aimed to find genetic
patterns associated with the serological classification of Leptospira bacteria by analyzing the
genetic composition of the rfb locus and proposing methods that allow for the classification of
Leptospira samples at the serogroup level. To do this, we used genomic data from 67 species
classified into 27 serogroups, which are distributed in 722 samples available in public
databases. We identified the genes that are part of the rfb locus through orthologous groups in
samples that contained the intact rfb locus in a single contig. We used a hierarchical clustering
method to group samples with similar genetic profiles of the rfb locus. This analysis made it
possible to contemplate the diversity of the genetic composition profile of the rfb locus in the
genus Leptospira and to observe correspondence between serogroup classification and the
groups formed by hierarchical clustering. The generated clustering suggests the classification
of samples into six large classes that, in addition to presenting serological affinity, share
similarities in the genetic composition of the rfb locus. It was observed that samples of the
same serogroup share similarities in the genetic composition of the rfb locus. Additionally, it
was possible to verify the existence of different gene blocks that may be conserved in samples
belonging to different species and serogroups. It is presumed that different combinations of
these gene blocks result in the synthesis of different O-antigen structures of
lipopolysaccharides and consequently different serogroups. This study allows for the suggestion of molecular markers that allow for the use of molecular strategies for the
serological identification of Leptospira. |
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