Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona

The intestinal microbiota may undergo changes due to the damage caused by chemotherapy treatment, facilitating the dissemination of opportunistic multiresistant microorganisms resistant to antimicrobials during the treatment of cancer patients. In this context, the objective of this study was to e...

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Autor principal: Coutinho, Joanny Rayane da Silva
Outros Autores: Medeiros, Lilian Giotto Zaros de
Formato: Dissertação
Idioma:pt_BR
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
Microbiota intestinal
Câncer
Resistência
Tratamento quimioterápico
Bactérias oportunistas
CNPQ::CIENCIAS BIOLOGICAS
Endereço do item:https://repositorio.ufrn.br/handle/123456789/52688
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id ri-123456789-52688
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language pt_BR
topic Microbiota intestinal
Câncer
Resistência
Tratamento quimioterápico
Bactérias oportunistas
CNPQ::CIENCIAS BIOLOGICAS
spellingShingle Microbiota intestinal
Câncer
Resistência
Tratamento quimioterápico
Bactérias oportunistas
CNPQ::CIENCIAS BIOLOGICAS
Coutinho, Joanny Rayane da Silva
Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
description The intestinal microbiota may undergo changes due to the damage caused by chemotherapy treatment, facilitating the dissemination of opportunistic multiresistant microorganisms resistant to antimicrobials during the treatment of cancer patients. In this context, the objective of this study was to evaluate the susceptibility of bacterial isolates obtained from the feces of cancer patients treated at a Reference Center in the state of Rio Grande do Norte against the antibacterials ciprofloxacin and ceftriaxone. For this, all research participants answered a questionnaire about socioeconomic conditions, eating habits and hygiene and use of antibiotics and the stool samples obtained were sown on Nutrient Agar (AN), Macconkey Agar (MC) and Salmonella- Shigella (SS) and performed the MIC and MBC of the antimicrobials ciprofloxacin and ceftriaxone considering concentrations from 0.06μg/mL to 128μg/mL. The collected data were analyzed using chi-square test, ANOVA and Newman-Keusl test. Most patients declared themselves white (60%) female (93%), urban area residents (93%), had completed high school (30%) and most (50%) used the antibiotic amoxicillin before beginning of the study. The most frequent age group observed was between 46 and 65 years (53.3%) and 83.3% of the patients had breast cancer. Regarding the stage of cancer, 30% had cancer in stage III of the disease and 33.3% were unable to inform, with a higher symptomatological incidence of vomiting (88.7%), followed by diarrhea (66.7%) and nausea ( 50%). Considering the MIC, the drug ciprofloxacin showed inhibitory activity in 90% of the isolates at a concentration of 8 μg/mL (MIC90) and 50% inhibition at a concentration of 2 μg/mL (MIC50). Regarding ceftriaxone, the bacterial samples showed MIC with inhibitory activity of 100% of the isolates at a concentration of 4 μg/mL (MIC90) and 70.4% inhibition at concentrations of 2 μg/mL and 0.5 μg/mL (MIC50). In the control group, the MIC was 98% inhibited by the drug ciprofloxacin and 100% by the drug ceftriaxone (MIC90), confirming the change in the MIC of fecal bacterial samples from cancer patients. It is concluded that the bacterial isolates obtained from cancer patients undergoing chemotherapy treatment showed a higher percentage of resistance to the tested antimicrobials when compared to the group of control individuals.
author2 Medeiros, Lilian Giotto Zaros de
author_facet Medeiros, Lilian Giotto Zaros de
Coutinho, Joanny Rayane da Silva
format masterThesis
author Coutinho, Joanny Rayane da Silva
author_sort Coutinho, Joanny Rayane da Silva
title Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
title_short Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
title_full Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
title_fullStr Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
title_full_unstemmed Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
title_sort susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona
publisher Universidade Federal do Rio Grande do Norte
publishDate 2023
url https://repositorio.ufrn.br/handle/123456789/52688
work_keys_str_mv AT coutinhojoannyrayanedasilva susceptibilidadebacterianadamicrobiotaentericadepacientesoncologicosfrenteaciprofloxacinaeceftriaxona
_version_ 1773960872033517568
spelling ri-123456789-526882023-06-13T23:06:04Z Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona Coutinho, Joanny Rayane da Silva Medeiros, Lilian Giotto Zaros de http://lattes.cnpq.br/9394771968259142 https://orcid.org/0000-0003-0167-6451 http://lattes.cnpq.br/6775535046477169 Motta Neto, Renato http://lattes.cnpq.br/6909091962347443 Melo, Maria Celeste Nunes de https://orcid.org/0000-0002-9826-4981 http://lattes.cnpq.br/0580551464788795 Aires, Caio Augusto Martins Microbiota intestinal Câncer Resistência Tratamento quimioterápico Bactérias oportunistas CNPQ::CIENCIAS BIOLOGICAS The intestinal microbiota may undergo changes due to the damage caused by chemotherapy treatment, facilitating the dissemination of opportunistic multiresistant microorganisms resistant to antimicrobials during the treatment of cancer patients. In this context, the objective of this study was to evaluate the susceptibility of bacterial isolates obtained from the feces of cancer patients treated at a Reference Center in the state of Rio Grande do Norte against the antibacterials ciprofloxacin and ceftriaxone. For this, all research participants answered a questionnaire about socioeconomic conditions, eating habits and hygiene and use of antibiotics and the stool samples obtained were sown on Nutrient Agar (AN), Macconkey Agar (MC) and Salmonella- Shigella (SS) and performed the MIC and MBC of the antimicrobials ciprofloxacin and ceftriaxone considering concentrations from 0.06μg/mL to 128μg/mL. The collected data were analyzed using chi-square test, ANOVA and Newman-Keusl test. Most patients declared themselves white (60%) female (93%), urban area residents (93%), had completed high school (30%) and most (50%) used the antibiotic amoxicillin before beginning of the study. The most frequent age group observed was between 46 and 65 years (53.3%) and 83.3% of the patients had breast cancer. Regarding the stage of cancer, 30% had cancer in stage III of the disease and 33.3% were unable to inform, with a higher symptomatological incidence of vomiting (88.7%), followed by diarrhea (66.7%) and nausea ( 50%). Considering the MIC, the drug ciprofloxacin showed inhibitory activity in 90% of the isolates at a concentration of 8 μg/mL (MIC90) and 50% inhibition at a concentration of 2 μg/mL (MIC50). Regarding ceftriaxone, the bacterial samples showed MIC with inhibitory activity of 100% of the isolates at a concentration of 4 μg/mL (MIC90) and 70.4% inhibition at concentrations of 2 μg/mL and 0.5 μg/mL (MIC50). In the control group, the MIC was 98% inhibited by the drug ciprofloxacin and 100% by the drug ceftriaxone (MIC90), confirming the change in the MIC of fecal bacterial samples from cancer patients. It is concluded that the bacterial isolates obtained from cancer patients undergoing chemotherapy treatment showed a higher percentage of resistance to the tested antimicrobials when compared to the group of control individuals. A microbiota intestinal pode sofrer alterações devido aos danos causados pelo tratamento quimioterápico, facilitando a disseminação de microrganismos oportunistas multirresistentes a antimicrobianos durante o tratamento de pacientes oncológicos. Nesse contexto, o objetivo deste estudo foi avaliar a susceptibilidade de isolados bacterianos obtidos de fezes de pacientes oncológicos atendidos em um Centro de Referência no estado do Rio Grande do Norte frente aos antibacterianos ciprofloxacina e ceftriaxona. Para isso, todos os participantes da pesquisa responderam um questionário sobre as condições socioeconômicas, hábitos alimentares e de higiene e uso de antibióticos e as amostras de fezes obtidas foram semeadas nos meios Ágar Nutriente (AN), Ágar Macconkey (MC) e Ágar Salmonella-Shigella (SS) e realizado a CIM e a CBM dos antimicrobianos ciprofloxacina e ceftriaxona considerando as concentrações de 0,06μg/mL à 128μg/mL. Os dados coletados foram analisados usando teste qui-quadrado, ANOVA e teste de Newman-Keusl. A maioria dos pacientes se declararam brancos (60%) do sexo feminino (93%), moradores da zona urbana (93%), possuíam ensino médio completo (30%) e a maioria (50%) fez uso do antibiótico amoxicilina antes do início do estudo. A faixa etária mais frequente observada foi entre 46 a 65 anos (53,3%) e 83,3% dos pacientes apresentaram câncer de mama. Em relação ao estágio de câncer, 30% apresentaram câncer em estágio III da doença e 33,3% não soube informar, havendo maior incidência sintomatológica de vômito (88,7%), seguido de diarreia (66,7%) e náusea (50%). Considerando a CIM, o fármaco ciprofloxacina apresentou atividade inibitória em 90% dos isolados na concentração de 8 μg/mL (CIM90) e 50% de inibição na concentração de 2 μg/mL (CIM50) . Em relação à ceftriaxona, as amostras bacterianas demonstraram a CIM com atividade inibitória de 100% dos isolados na concentração de 4 μg/mL (CIM90) e 70,4% de inibição nas concentrações de 2 μg/mL e 0,5 μg/mL (CIM50). No grupo controle a CIM teve inibição 98% no fármaco ciprofloxacina e 100% no fármaco ceftriaxona (CIM90), comprovando a alteração da CIM das amostras bacterianas fecais dos pacientes oncológicos. Conclui-se que os isolados bacterianos obtidos de pacientes oncológicos submetidos a tratamento quimioterápicos apresentaram um percentual mais elevado de resistência aos antimicrobianos testados quando comparado ao grupo de indivíduos controles. 2023-06-13T23:05:29Z 2023-06-13T23:05:29Z 2023-02-27 masterThesis COUTINHO, Joanny Rayane da Silva. Susceptibilidade bacteriana da microbiota entérica de pacientes oncológicos frente a ciprofloxacina e ceftriaxona. Orientador: Lilian Giotto Zaros de Medeiros. 2023. 73f. Dissertação (Mestrado em Biologia Parasitária) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2023. https://repositorio.ufrn.br/handle/123456789/52688 pt_BR Acesso Aberto application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM BIOLOGIA PARASITÁRIA

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