Eficácia e segurança do extrato de sementes de Angico (Anadenanthera colubrina) contra Aedes aegypti e Aedes albopictus, em condições de laboratório e de campo
Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) are etiological agent vectors of numerous arboviruses of medical importance. Limitations in chemical insecticides prompted the search for effective and ecologically friendly options, such as botanical insecticides. The present study describ...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/52184 |
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| Resumo: | Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) are etiological agent
vectors of numerous arboviruses of medical importance. Limitations in chemical
insecticides prompted the search for effective and ecologically friendly options,
such as botanical insecticides. The present study describes the aedicidal potential
of Anadenanthera colubrina (Vell.) seed extract at different stages of the vectors'
life cycle, its attractive/repellent action on oviposition, partial biochemical
characterization and evaluation of cyto- and ecotoxicity. The hot aqueous extract
of A. colubrina seeds (EAQA) was obtained in distilled water extraction (1:10, m/v)
for 7 h at 80 °C. The larvicidal effect of EAQA was evaluated in serial dilutions,
determining the lethal LC50 and LC90 concentrations (mg/mL) after 24 h and 48 h
of exposure. The larvicidal activity for A. aegypti and A. albopictus recorded CL50
and CL90 at 24 h (0.16 and 0.94 mg/mL; 0.36 and 1.28 mg/mL) and 48 h (0. 02
and 0.26 mg/mL; 0.06 and 0.62 mg/mL), respectively. EAQA was pupicid for both
insects at concentrations higher than larvicidal concentrations (24 h LC50 ≥ 0.85
mg/mL and 48 h LC50 ≥ 0.53 mg/mL). 0.26 mg/mL was chosen as the diagnostic
concentration for ovicidal activity and oviposition, showing ovicidal activity for both
insects, compromising larval hatching by 76% and reducing adult emergence of
surviving larvae by 25%. Under field conditions, EAQA exerts an attractive effect
on oviposition. EAQA showed a higher concentration of total carbohydrates (15.5
mg/mL) compared to total soluble protein (2.0 mg/mL) and total phenolic
compounds (0.1 mg/mL). Bromelain inhibitors, papain, trypsin, and chymotrypsin,
were observed in EAQA with enzyme inhibition activity in 90.94%, 68.93%,
68.75%, and 9.32%, respectively. Cytotoxicity was not detected for the cell lines
tested (3T3) and (HepG2), nor ecotoxicity for the microcrustaceans Ceriodaphnia
dubia and Mysidopsis juniae. EAQA has the potential to be an adjuvant in Aedes
integrated management strategies due to the versatility of action against different
stages of the vectors' life cycle, as well as their cyto- and eco-compatibility. |
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