Análise genômica de microrganismos degradadores de hidrocarbonetos do petróleo e seu potencial de atuação em hidrocarbonetos prioritários
Contamination of soils and marine ecosystems by hydrocarbons that constitute petroleum from large and small oil spills throughout its supply chain brings serious consequences to the environment. Among the existing strategies to mitigate environmental impacts in affected areas, bioremediation by...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/52138 |
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| Resumo: | Contamination of soils and marine ecosystems by hydrocarbons that constitute
petroleum from large and small oil spills throughout its supply chain brings
serious consequences to the environment. Among the existing strategies to
mitigate environmental impacts in affected areas, bioremediation by
bioaugmentation using organisms capable of degrading oil is an alternative that
offers a better cost-benefit ratio and promotes greater removal of compounds
when compared to physical-chemical methods. National and international
environmental regulatory agencies list 179 compounds as priority for
bioremediation due to their toxic and/or mutagenic potential. From previous
works, the members of the research group of the Laboratory of Molecular Biology
and Genomics have been obtaining bacterial isolates from samples of
environments contaminated by oil, maintaining a stock of these isolates that
compose a bank of microorganisms preserved by the laboratory. The genomes
of isolates with promising profile to act in bioremediation are being sequenced,
in an attempt to identify their taxonomic and metabolic profile. So far, through
the sequencing of the complete genome of 22 bacterial isolates previously
obtained by the group and sequencing of the 16S gene of 18 isolates obtained
from oil samples collected on beaches on Rio Grande do Norte in the development
of this work, resulted in the identification of 10 genera of bacteria able to grow
using oil as a carbon source. The analysis of the generated data, using the R
programming language, allowed the comparison with their respective reference
genomes, determining their relationships and particularities. It was identified
among all isolates with complete genome sequenced 53 genes that encode
enzymes, present in 20 pathways of degradation and metabolism of xenobiotics
from KEGG, which participate in the degradation process of 37 hydrocarbons
reported as priority, as well as the similarities of the degradation profile of the
isolates. Through in silico analysis, a consortium of 4 isolates was proposed with
potential to act in bioremediation of 34 of the 37 compounds. |
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