Estudo de produção, caracterização e biocompatibilidade in vitro de nanocápsulas poliméricas contendo óleo de copaíba em células pulmonares
Copaiba oil has been largely used due to its therapeutic properties. Nanocapsules were revealed to be a great nanosystem to carry natural oils due to their ability to improve the bioaccessibility and the bioavailability of lipophilic compounds. The aim of this study was to produce and characteriz...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/52036 |
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| Resumo: | Copaiba oil has been largely used due to its therapeutic properties. Nanocapsules
were revealed to be a great nanosystem to carry natural oils due to their ability to
improve the bioaccessibility and the bioavailability of lipophilic compounds. The aim of
this study was to produce and characterize copaiba oil nanocapsules (CopNc) and to
evaluate their hemocompatibility, cytotoxicity, and genotoxicity. Copaiba oil was
chemically characterized by GC-MS and FTIR. CopNc was produced using the
nanoprecipitation method. The physicochemical stability, toxicity, and biocompatibility
of the systems, in vitro, were then evaluated. β-bisabolene, cis-α-bergamotene,
caryophyllene, and caryophyllene oxide were identified as the major copaiba oil
components. CopNc showed a particle size of 215 ± 10 nm, a polydispersity index of
0.15 ± 0.01, and a zeta potential of −18 ± 1. These parameters remained unchanged
over 30 days at 25 ± 2 °C. The encapsulation efficiency of CopNc was 54 ± 2%. CopNc
neither induced hemolysis in erythrocytes, nor cytotoxic and genotoxic effects in lung
cells at the range of concentrations from 50 to 200 μg·mL−1
. In conclusion, CopNc
showed suitable stability and physicochemical properties. Moreover, this formulation
presented a remarkable safety profile on lung cells. These results may pave the way
to further use CopNc for the development of phytotherapeutic medicine intended for
pulmonary delivery of copaiba oil. |
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