Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante
The red seaweed Gracilaria birdiae (Gb) is cultivated and used as food in Northeast Brazil and its sulfated polysaccharide (agaran), called SPGb, is said to contain several properties of academic interest, including as an antioxidant agent. Chromium picolinate (ChrPic) is a bioinorganic compound...
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Estresse oxidativo Nutracêutico Gracilaria birdiae Micronúcleo Agarana CNPQ::CIENCIAS BIOLOGICAS |
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Estresse oxidativo Nutracêutico Gracilaria birdiae Micronúcleo Agarana CNPQ::CIENCIAS BIOLOGICAS Morais, Yara Campanelli de Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
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The red seaweed Gracilaria birdiae (Gb) is cultivated and used as food in
Northeast Brazil and its sulfated polysaccharide (agaran), called SPGb, is said to
contain several properties of academic interest, including as an antioxidant agent.
Chromium picolinate (ChrPic) is a bioinorganic compound with anti-inflammatory
capacity. The objective was to produce blends containing SPGb and ChrPic and
evaluate them as better antioxidant agents, since blends correspond to the association
of compounds whose objective is to improve their activity/efficiency, in relation to
isolated producers. Regarding the properties attributed to SPGb and ChrPic, there is
no record of blends uniting them. ChrPic was purchased commercially and SPGb
obtained from seaweed, after an extraction technique through exposure to ultrasound
waves, basic medium and proteolysis. The identification of SPGb was confirmed by
chemical analysis and nuclear magnetic resonance (1H1D and HSQC). ChrPic (from
0.5 to 2.0 mg/mL) did not show copper or iron chelating activity, whereas SPGb (2.0
mg/mL) showed activity of 70 and 73% for these tests, respectively. ChrPic (1.0
mg/mL) showed approximately 80% and 100% scavenging activity for superoxide and
hydroxyl radicals, respectively, while SPGb (2.0 mg/mL) did not show this activity. Five
blends were produced (B1; B2; B3; B4; B5) whose antioxidant activity was evaluated
and data indicated B5 as the blend with the most potent antioxidant agent.
Micronucleus test (CBMN) demonstrated that B5 does not have genotoxic activity, as
well as for cytotoxicity in murine fibroblasts (3T3) and Chinese hamster ovary cells
(CHO-K1), B5 and SPGb also did not show genotoxic activity, ChrPic (0.2 mg/mL)
decreased the ability of cells to reduce MTT by 30%, but all samples protected cells by
more than 70%. When 3T3 cells were exposed to hydrogen peroxide (H2O2) (0.6 mM)
they showed about 50% of the ability to reduce MTT compared to cells not exposed to
peroxide, so three different moments of H2O2 presence were tested. In the first
moment, cells were incubated with peroxide and subsequently exposed to SPGb,
ChrPic or B5 for 24 hours. In this case, SPGb was not effective in protecting cells from
peroxide, while cells exposed to ChrPic (0.025; 0.05 and 0.1 mg/mL) and B5 (0.05
mg/mL) reduced MTT by 100%. In the second moment, the samples were placed in
the cells along with hydrogen peroxide and it was noticed that a reduction of less than
30% of MTT was observed in the presence of SPGb, of up to 55% in the presence of
ChrPic, and of 96% in the presence of B5 (0.05 mg/mL). In the third moment, the cells
were exposed to SPGb, ChrPic or B5 for 24 h, and subsequently to H2O2 (0.6 mM). It
was verified that the presence of SPGb was unable to prevent the decrease in the
capacity of the cells in reducing MTT, while cells exposed to ChrPic and B5, at all
concentrations, reduced MTT by 100%. Blend B5 is a potential antioxidant agent and
more in vivo studies are needed to confirm its nutraceutical activity. |
| author2 |
Rocha, Hugo Alexandre de Oliveira |
| author_facet |
Rocha, Hugo Alexandre de Oliveira Morais, Yara Campanelli de |
| format |
doctoralThesis |
| author |
Morais, Yara Campanelli de |
| author_sort |
Morais, Yara Campanelli de |
| title |
Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
| title_short |
Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
| title_full |
Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
| title_fullStr |
Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
| title_full_unstemmed |
Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
| title_sort |
blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante |
| publisher |
Universidade Federal do Rio Grande do Norte |
| publishDate |
2023 |
| url |
https://repositorio.ufrn.br/handle/123456789/51337 |
| work_keys_str_mv |
AT moraisyaracampanellide blendsdepolissacarideossulfatadosepicolinatodecromoproducaoeavaliacaodesuaatividadeantioxidante |
| _version_ |
1773957769509994496 |
| spelling |
ri-123456789-513372023-02-23T16:49:08Z Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante Morais, Yara Campanelli de Rocha, Hugo Alexandre de Oliveira http://lattes.cnpq.br/9254975868645972 https://orcid.org/0000-0003-2252-1221 http://lattes.cnpq.br/4651814546820796 Moura, Carlos Eduardo Bezerra de Fidelis, Gabriel Pereira Macedo, Marina de Oliveira Cardoso Santos, Pablo de Castro http://lattes.cnpq.br/6456286305129050 Estresse oxidativo Nutracêutico Gracilaria birdiae Micronúcleo Agarana CNPQ::CIENCIAS BIOLOGICAS The red seaweed Gracilaria birdiae (Gb) is cultivated and used as food in Northeast Brazil and its sulfated polysaccharide (agaran), called SPGb, is said to contain several properties of academic interest, including as an antioxidant agent. Chromium picolinate (ChrPic) is a bioinorganic compound with anti-inflammatory capacity. The objective was to produce blends containing SPGb and ChrPic and evaluate them as better antioxidant agents, since blends correspond to the association of compounds whose objective is to improve their activity/efficiency, in relation to isolated producers. Regarding the properties attributed to SPGb and ChrPic, there is no record of blends uniting them. ChrPic was purchased commercially and SPGb obtained from seaweed, after an extraction technique through exposure to ultrasound waves, basic medium and proteolysis. The identification of SPGb was confirmed by chemical analysis and nuclear magnetic resonance (1H1D and HSQC). ChrPic (from 0.5 to 2.0 mg/mL) did not show copper or iron chelating activity, whereas SPGb (2.0 mg/mL) showed activity of 70 and 73% for these tests, respectively. ChrPic (1.0 mg/mL) showed approximately 80% and 100% scavenging activity for superoxide and hydroxyl radicals, respectively, while SPGb (2.0 mg/mL) did not show this activity. Five blends were produced (B1; B2; B3; B4; B5) whose antioxidant activity was evaluated and data indicated B5 as the blend with the most potent antioxidant agent. Micronucleus test (CBMN) demonstrated that B5 does not have genotoxic activity, as well as for cytotoxicity in murine fibroblasts (3T3) and Chinese hamster ovary cells (CHO-K1), B5 and SPGb also did not show genotoxic activity, ChrPic (0.2 mg/mL) decreased the ability of cells to reduce MTT by 30%, but all samples protected cells by more than 70%. When 3T3 cells were exposed to hydrogen peroxide (H2O2) (0.6 mM) they showed about 50% of the ability to reduce MTT compared to cells not exposed to peroxide, so three different moments of H2O2 presence were tested. In the first moment, cells were incubated with peroxide and subsequently exposed to SPGb, ChrPic or B5 for 24 hours. In this case, SPGb was not effective in protecting cells from peroxide, while cells exposed to ChrPic (0.025; 0.05 and 0.1 mg/mL) and B5 (0.05 mg/mL) reduced MTT by 100%. In the second moment, the samples were placed in the cells along with hydrogen peroxide and it was noticed that a reduction of less than 30% of MTT was observed in the presence of SPGb, of up to 55% in the presence of ChrPic, and of 96% in the presence of B5 (0.05 mg/mL). In the third moment, the cells were exposed to SPGb, ChrPic or B5 for 24 h, and subsequently to H2O2 (0.6 mM). It was verified that the presence of SPGb was unable to prevent the decrease in the capacity of the cells in reducing MTT, while cells exposed to ChrPic and B5, at all concentrations, reduced MTT by 100%. Blend B5 is a potential antioxidant agent and more in vivo studies are needed to confirm its nutraceutical activity. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq A alga vermelha Gracilaria birdiae (Gb) é cultivada e usada como alimento no Nordeste do Brasil e seu polissacarídeo sulfatado (agarana), aqui denominado de SPGb, é apontado contendo várias propriedades de interesse acadêmico, inclusive como agente antioxidante. Já o picolinato de cromo (ChrPic) é um composto bioinorgânico com capacidade anti-inflamatória descrita recentemente, sendo assim, teve-se como objetivo produzir blends contendo SPGb e ChrPic e avaliá-los como melhores agentes antioxidantes inicialmente, pois blends correspondem a associação de compostos cujo objetivo é melhorar sua atividade/eficiência, em relação aos produtores isolados. Em relação as propriedades atribuídas ao SPGb e ChrPic, não há registro de blends unindo os mesmos. O ChrPic foi adquirido comercialmente e o SPGb obtido da alga após técnica de extração através de exposição a ondas de ultrassom, meio básico e proteólise. A identificação do SPGb foi confirmada por análises químicas e de ressonância magnética nuclear (1H1D e HSQC). O ChrPic (de 0,5 a 2,0 mg/mL) não apresentou atividade quelante de cobre ou de ferro, já o SPGb (2,0 mg/mL) apresentou atividade de 70 e 73%, para esses testes, respectivamente. Por outro lado, ChrPic (1,0 mg/mL) apresentou cerca 80% e 100% de atividade de sequestro dos radicais superóxido e hidroxila, respectivamente, enquanto SPGb (2,0 mg/mL) não apresentou essa atividade. Foram produzidos 5 blends (B1; B2; B3; B4; B5) cuja atividade antioxidante foi avaliada e dados indicaram B5 como o blend mais potente agente antioxidante. Teste de micronúcleo (CBMN) demonstrou que B5 não possui atividade genotóxica, assim como, para citotoxicidade em fibroblastos murínico (3T3) e células de ovário de hamster chinês (CHO-K1), o B5 e o SPGb também não apresentaram atividades, o ChrPic (0,2 mg/mL) diminuiu em 30% a capacidade das células em reduzir o MTT, porém todas as amostras protegeram as células em mais de 70%. Quando células 3T3 foram expostas a peróxido de hidrogênio (H2O2) (0,6 mM) apresentaram cerca 50% da capacidade de reduzir o MTT em comparação com células não expostas ao peróxido, foram então testados três momentos diferentes de presença de H2O2, sendo o primeiro um reparador, quando as células foram incubadas com peróxido e posteriormente ficaram expostas a SPGb, ChrPic ou B5 por 24 horas. Nesse caso o SPGb não foi efetivo em proteger as células do peróxido, enquanto as células expostas a ChrPic (0,025; 0,05 e 0,1 mg/mL) e B5 (0,05 mg/mL) reduziram em 100% o MTT; já no segundo momento, o concomitante, quando as amostras foram colocadas nas células juntamente com o peróxido de hidrogênio e percebeu-se uma redução de menos de 30% do MTT na presença de SPGb, de até 55% na presença de ChPic e de 96% na presença de B5 (0,05 mg/mL). No terceiro momento, o preventivo, as células foram expostas ao SPGb, ChrPic ou B5 por 24 h, e posteriormente ao H2O2 (0,6 mM), verificou-se que a presença de SPGb foi incapaz de impedir a diminuição da capacidade das células em reduzir o MTT, enquanto as células expostas ao ChrPic e ao B5, em todas as concentrações, reduziram em 100% o MTT. Os dados demonstraram que B5 possui atividade antioxidante e possivelmente utilizado em teste futuros in vivo, para confirmar a sua ação antioxidante e assim indicá-lo como nutracêutico. 2023-02-23T16:48:29Z 2023-02-23T16:48:29Z 2022-11-30 doctoralThesis MORAIS, Yara Campanelli de. Blends de polissacarídeos sulfatados e picolinato de cromo: produção e avaliação de sua atividade antioxidante. Orientador: Hugo Alexandre de Oliveira Rocha. 2022. 99f. Tese (Doutorado em Bioquímica e Biologia Molecular) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2022. https://repositorio.ufrn.br/handle/123456789/51337 pt_BR Acesso Aberto application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM BIOQUÍMICA E BIOLOGIA MOLECULAR |