Avaliação da mutagenicidade de microesferas à base de hidroxiapatita nanoestruturada pelo teste de reversão da Salmonella typhimurium
Bone injuries caused by degenerative and infectious diseases, traumas or disorders are a challenge for current medicine, as the treatment considered the “gold standard” - autologous transplants - presents some limitations. Hydroxyapatite (HA) is a bioceramic material already known for its osteoinduc...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/51325 |
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| Resumo: | Bone injuries caused by degenerative and infectious diseases, traumas or disorders are a challenge for current medicine, as the treatment considered the “gold standard” - autologous transplants - presents some limitations. Hydroxyapatite (HA) is a bioceramic material already known for its osteoinductive and osteoconductive potential that allows changes in its structure and composition in order to enhance its regenerative capabilities. Thus, the Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa (REGENERA) developed microspheres based on nanostructured HA containing substitution with different ions aiming at optimizing these materials for future clinical applications. Regulatory authorities, such as the Organization for Economic Cooperation and Development (OECD), request the assessment of mutagenicity as one of the requirements to certify the biocompatibility of drugs and biomaterials, being the reversion test in Salmonella typhimurim one of the most suitable for this purpose. To analyze the mutagenic potential of nanostructured HA-based microspheres, strains TA100 and TA98 were used in this study. Initially, the strains were evaluated for their spontaneously reversion and mutagens-induced reversion rates , followed the established protocols. The cells were exposed to the samples, in the presence and absence of the lysosomal extract of mouse liver cells (fraction S9,) in order to evaluate the mutagenicity of the metabolization products of the samples. The results indicate the absence of mutagenicity of the samples in both strains, with and without treatment with the S9 fraction, with a mutagenicity ratio (RM) of less than 2 being observed in all assays. |
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