Imunoexpressão de ING 4, VEGF e NF-κB em lesões periapicais inflamatórias
Inflammatory periapical lesions (IPLs) are pathological conditions resulting from infections of odontogenic origin, being mainly represented by periapical granulomas (PGs) and radicular cysts (RCs). Its pathogenesis is associated with immunological and angiogenic mechanisms. This retrospective st...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/50911 |
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| Resumo: | Inflammatory periapical lesions (IPLs) are pathological conditions resulting from infections
of odontogenic origin, being mainly represented by periapical granulomas (PGs) and radicular
cysts (RCs). Its pathogenesis is associated with immunological and angiogenic mechanisms.
This retrospective study, semi-quantitative and comparative aimed to analyze the
immunohistochemical expression of ING-4, VEGF and NF-κB in IPLs, and to correlate the
pattern of expression of these proteins. The sample consisted of 26 were PGs, 17 RCs and 19
residual radicular cysts (RRCs). Epithelial thickness and inflammatory infiltrate were
evaluated, and the correlation of these findings with the expression pattern of ING-4, VEGF
and NF-κB proteins in selected IPLs. To perform the statistical analysis, the chi-square,
Kruskal-Wallis, Mann-Whitney and Spearman correlation tests were used (p < 0.05). The
inflammatory infiltrate exhibited greater intensity in the PG, followed by the RC, and finally,
the RRC (p < 0.05). Although there was no statistically significant association when
associating the expression of ING-4 in inflammatory cells of the connective tissue or fibrous
capsule the groups of IPLs, the PG and RC showed higher expression of this protein. There
was no statistically significant association between ING-4 and the intensity of the
inflammatory infiltrate. Immunoexpression of VEGF in the nucleus of inflammatory cells in
the connective tissue or fibrous capsule shows a significant association with IPLs, in which
there is greater expression of this protein occurring in cysts (p= 0,002). The highest
expression of NF-κB was evidenced in cases of PGs, both at the nuclear and cytoplasmic level
of inflammatory cells (p=0,005; p= 0,002). There was no statistically significant association
when comparing the expression of NF-κB between the cysts, but the median expression of
this protein was expression was higher for the RCs. In the fibrous capsule, nuclear and
cytoplasmic NF-κB immunoexpression in inflammatory cells was higher in periapical lesions
with intense inflammatory infiltrate (p<0.001). Therefore, it is suggested that ING-4, VEGF
and NF-κB participate in the etiopathogenesis of IPLs, and that there is a directly proportional
relationship between the expression of these proteins. ING-4 did not exert regulatory activity
in the inflammation associated with these lesions. |
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