Efeito protetor de Lacticaseibacillus casei no modelo de mucosite intestinal induzida por 5-fluorouracil em camundongos
Mucositis in the gastrointestinal tract is an inflammation of the mucosa that has as one of the causes the administration of 5-Fluorouracil (5-FU) in anticancer therapy. There is still no elucidated treatment for the cure or prophylaxis of mucositis. In this context, the present work's objec...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/50842 |
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| Resumo: | Mucositis in the gastrointestinal tract is an inflammation of the mucosa that has as one of
the causes the administration of 5-Fluorouracil (5-FU) in anticancer therapy. There is still
no elucidated treatment for the cure or prophylaxis of mucositis. In this context, the
present work's objective was to evaluate the effect of oral administration of
Lacticaseibacilus casei (L. casei) in an animal model of intestinal mucositis. Initially,
Lacticaseibacilus casei (1x109 CFU/mL) or saline was orally administered to Swiss mice
starting 15 days before induction of mucositis by a single intraperitoneal administration
of 5-FU (450mg/kg). After euthanasia, on day 18th, tissue samples from the colon and
each segment of the small intestine were collected for histopathology. The jejunal tissues
present iNOS and TNF-α immunoexpression, IL-1β, IL-6, and TNF-α levels,
malondialdehyde (MDA) accumulation, and nuclear factor kappa B gene expression (NFκB P65), toll receptor -like 4 (TLR-4), mucin-2 (MUC-2), occludin (OCLN) and zonula
occludent-1 (ZO-1). L. casei reduced 5-FU-induced inflammation in the colon and small
intestine (p<0.05), the levels of TNF-α, IL-1beta, IL-6 (p<0.05), and MDA (p< 0.05), as
well as the expression of iNOS and TNF-α proteins (p<0.05). Furthermore, the gene
expression of OCLN and ZO-1 was upregulated (p<0.05). In contrast, the gene expression
of NF-κB P65 and TLR4 (p<0.05) was downregulated. The L.casei group also showed a
higher population of lactic acid bacteria (p<0.05) compared to the control groups. Thus,
the oral administration of L.casei demonstrated a potential protective effect against 5-FUinduced intestinal mucositis in Swiss mice. |
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