Indução de imunoglobulina g total frente a antígenos quiméricos recombinantes de Trypanosoma cruzi associados a diferentes adjuvantes vacinais
Trypanosoma cruzi protozoan infections affect humans by causing Chagas disease (CD). Currently, there are no prophylactic or therapeutic vaccines in the context of CD, and the discovery of possible protozoan antigenic candidates may improve immunogenicity in vaccine strategies. The main of this stud...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/50533 |
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| Resumo: | Trypanosoma cruzi protozoan infections affect humans by causing Chagas disease (CD). Currently, there are no prophylactic or therapeutic vaccines in the context of CD, and the discovery of possible protozoan antigenic candidates may improve immunogenicity in vaccine strategies. The main of this study main was to use a murine model of immunization to evaluate the total IgG antibody profile of four recombinant chimeric Trypanosoma cruzi proteins. In that order, a mix associated or not with the adjuvants, such as aluminum hydroxide and incomplete Freund's – AIF, were used. Besides, the characterization of three structural polypeptides (VP1, VP2, VP3) immunostimulatory capacity also was performed. BALB/c mice were immunized,
and blood samples were collected at 15, 30, and 45 days after primary immunization. Thus, the immune profile was evaluated using an indirect Enzyme-Linked Immunosorbent Assay (ELISA). Results demonstrated a higher production of total anti-IBMP-8.1, 8.2, 8.3, and 8.4 IgG when associated with the AIF-associated group (Mix + AIF), and IBMP-8.3 and 8.4 proteins showed more immunogenicity isolated. For the activity of VPs with T. cruzi antigenic candidates, VP1 and VP3 demonstrated greater induction of total IgG. Further studies are needed to analyze secondary immunization, the humoral immune response of IgG subclasses, and cellular immune response through cytokines analysis. |
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