Perfil imuno-histoquímico de marcadores associados à transição epitelial-mesenquimal em lesões odontogênicas epiteliais benignas
Odontogenic cysts and tumors, lesions that affect the maxillomandibular complex, may exhibit a more aggressive clinical-biological behavior. And the epithelial-mesenchymal transition (EMT), a process by which epithelial cells lose phenotypic properties and acquire characteristics of mesenchymal c...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/49932 |
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| Resumo: | Odontogenic cysts and tumors, lesions that affect the maxillomandibular complex, may exhibit
a more aggressive clinical-biological behavior. And the epithelial-mesenchymal transition
(EMT), a process by which epithelial cells lose phenotypic properties and acquire
characteristics of mesenchymal cells, including increased motility and invasiveness, through
the regulation of central transcription factors and their associated pathways, may be part of
characteristics associated with odontogenic lesions. Thus, the present work sought to analyze
and compare the immunohistochemical expression of proteins (Zeb1, E-cadherin, N-cadherin
and vimentin) involved in the MET process in benign epithelial odontogenic lesions. The
sample consisted of 88 cases of odontogenic lesions, comprising 28 cases of ameloblastoma
(AB), 30 of odontogenic keratocyst (CO) and 30 of dentigerous cyst (CD). All specimens
submitted to the immunohistochemical technique were evaluated by light microscopy and
submitted to the random choice of 5 (five) fields, which were photographed at a magnification
of 400x. The evaluation of the expression of each marker, based on the analysis in its specific
cellular compartment, was carried out in a semi-quantitative manner, through the multiplication
of the scores associated with the percentage of immunostained cells by the scores related to the
intensity of staining, with an average of the five fields and the result defined as low expression
or high expression, according to the methodology used. The associations were made using the
chi-square test and the correlations using the Spearman correlation test. The significance level
was set at 5% (p < 0.05). The results showed a prevalence peak between the 2nd and 3rd decades
of life, in all the lesions studied, with a greater involvement in the posterior region of the
mandible, and the ABs were the largest lesions, with 65% measuring above 2, 5cm.
Immunohistochemistry showed low expression of Zeb1 in the odontogenic epithelium of the
lesions studied, high expression of E-cadherin, high expression of N-cadherin and an
intermediate expression of vimentin. When the correlation between the markers was performed, a positive and moderate correlation was observed in the cases of AB between nuclear Zeb1 and
membrane E-cadherin, cytoplasmic Zeb1 and membrane E-cadherin and between cytoplasmic
E-cadherin and vimentin. As well as a moderate positive correlation, in CD cases, between
nuclear Zeb1 and cytoplasmic vimentin, and between cytoplasmic Zeb1 and vimentin.
Therefore, we can conclude that Zeb1 may be acting indirectly on the pathways responsible for
the growth and morphological characteristics of these lesions studied. Furthermore, the
differential expression of E-cadherin, N-cadherin and vimentin was shown to be part of a partial
TEM process in the benign epithelial odontogenic lesions studied. |
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