Caracterização e estudo de efeitos antimicrobianos de nanopartículas de magnetita (Fe3O4)
The use of magnetic nanoparticles has emerged as a new alternative to combat antibiotic-resistant bacteria. Its main mechanism of action is to increase the oxidative stress in bacterial cells through the generation of reactive oxygen species and physical damage to the cell surface. In this perspecti...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/49340 |
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| Resumo: | The use of magnetic nanoparticles has emerged as a new alternative to combat antibiotic-resistant bacteria. Its main mechanism of action is to increase the oxidative stress in bacterial cells through the generation of reactive oxygen species and physical damage to the cell surface. In this perspective, we synthesized magnetite nanoparticles (IONP's) and demonstrated in vitro their influence on the growth of Staphylococcus aureus BMB9393 (MRSA) and Pseudomonas aeruginosa (multi-resistant), at a concentration of 12 mg/ml and at different exposure times, as also its action in the formation and in the formed biofilms. The IONP's were produced by the polyol method and characterized by XRD, VSM, FT-IR and zeta potential. Then, diluted bacterial suspensions were distributed in conical tubes: one group was subjected to contact with the nanoparticles and the other was exposed to only the culture medium (positive control), both left in an oven at 37°C. After 2, 6 and 8 hours, an aliquot of each was taken and pipetted into a 96-well plate for optical density (OD) reading. From the best exposure time, we performed the cell viability test using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). The antibiofilm assay was performed using crystal violet and the results obtained from the measurement of OD. The data were plotted in an Excel spreadsheet to perform the calculations and build the graphs. After 6h of exposure, the nanoparticle was able to inhibit 69% in S. aureus, but compared to the 2h exposure (79%), there was a 10% difference in growth inhibition, meaning a better result in this bacteria, since the difference in P. aeruginosa between 2h (78%) and 6h (80%) was only 2%. Both bacteria were poor biofilm producers, but the nanoparticles was able to inhibit formation by 19% in S. aureus and 14% in P. aeruginosa. While in the mature biofilm, the IONP's managed to decrease the biomass in 12% of S. aureus and in only 6% of P. aeruginosa. The data showed that the nanoparticles synthesized by the polyol method inhibited the growth of resistant bacteria, as well as demonstrated antibiofilm activity, placing them as a possible antimicrobial therapy. |
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