Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança

Tephrosia toxicaria (Sw.) Pers. is a legume occurring in the Northeast Brazil that has attracted the attention of researchers because it serves as a matrix to obtain numerous adjuvant formulations for integrated management strategies of vectors and diseases, such as plant extracts, due to its select...

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Autor principal: Silva, Giulian César da
Outros Autores: Uchoa, Adriana Ferreira
Formato: doctoralThesis
Idioma:pt_BR
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
Recursos naturais
Extratos botânicos
Timbó
Arboviroses
Estresse oxidativo
Endereço do item:https://repositorio.ufrn.br/handle/123456789/47244
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id ri-123456789-47244
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language pt_BR
topic Recursos naturais
Extratos botânicos
Timbó
Arboviroses
Estresse oxidativo
spellingShingle Recursos naturais
Extratos botânicos
Timbó
Arboviroses
Estresse oxidativo
Silva, Giulian César da
Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
description Tephrosia toxicaria (Sw.) Pers. is a legume occurring in the Northeast Brazil that has attracted the attention of researchers because it serves as a matrix to obtain numerous adjuvant formulations for integrated management strategies of vectors and diseases, such as plant extracts, due to its selective toxicity and environmental compatibility. The search for new natural adjuvant formulations for the management of diseases, vectors and pathogens shows that plant extracts are still promising, especially considering the great Brazilian biodiversity, they are obtained from renewable sources, their toxic selectivity to target organisms and environmental compatibility. The development of the present research was stimulated by the possibility of expanding the repertoire of options for natural adjuvants, which aimed to investigate the bioactive potential and biosecurity of aqueous and hydroethanolic extracts from seeds, roots, stems and leaves of T. toxicaria. The first chapter of this thesis deals with a bibliographic review of the current state of art on the use of botanical insecticides in the integrated management of arbovirus vectors, with emphasis on species belonging to the genera Aedes, Culex and Anopheles. The second chapter describes the versatility of T. toxicaria extracts in the Aedes control, highlighting the hydroethanolic root extract (RHA) as the most promising larvicidal extract, demonstrating the lowest lethal concentration for 50% (300 µg/mL) and 90% (840 µg/mL) of Aedes larvae, observed at 24 h post-exposure. In field tests, RHA (840 µg/mL) is a promising oviposition deterrent agent, reducing Aedes egg laying by approximately 90%. In addition to manifesting insecticidal properties, RHA manifests antioxidant activity, mainly in control of metal ion homeostasis and restoration of the metabolic activity of cells under oxidative stress conditions, under low concentrations (100 µg/mL). Although leishmanicidal molecules are also expressed in RHA, its solubilization was less pronounced, requiring a higher concentration of extract to inhibit the growth of Leishmania amazonensis promastigotes (IC50 = 3.53 mg/mL). In the concentration range of 1024 – 32 μg/mL, RHA was not able to inhibit the growth of the bacterial and fungal strains investigated. RHA concentrates carbohydrates, phenolic compounds and proteins, with estimated molecular masses between 10 and 24 kDa. Protein analyzes suggest the expression of protease inhibitors and lectins in RHA. In the concentration range of 300 – 1 μg/mL, RHA does not compromise the initial development and biomass of Lactuca sativa; and in the concentration range of 1000 – 1 μg/mL, RHA does not compromise the metabolic activity of HepG2 (CC50 = 2.68 mg/mL) and 3T3 (CC50 = 5.07 mg/mL). The promising bioinsecticidal and antioxidant effects, associated with the absence of toxicity events against non-target organisms and cell lines, suggest T. toxicaria as a natural source to be sustainably exploited to obtain extracts with wide biological and pharmacological use.
author2 Uchoa, Adriana Ferreira
author_facet Uchoa, Adriana Ferreira
Silva, Giulian César da
format doctoralThesis
author Silva, Giulian César da
author_sort Silva, Giulian César da
title Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
title_short Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
title_full Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
title_fullStr Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
title_full_unstemmed Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
title_sort extratos de tephrosia toxicaria (sw.) pers.: prospecção de atividades anti-aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança
publisher Universidade Federal do Rio Grande do Norte
publishDate 2022
url https://repositorio.ufrn.br/handle/123456789/47244
work_keys_str_mv AT silvagiuliancesarda extratosdetephrosiatoxicariaswpersprospeccaodeatividadesantiaedesantioxidanteantimicrobianaeleishmanicidaebiosseguranca
AT silvagiuliancesarda tephrosiatoxicariaswpersextractsprospectionofantiaedesantioxidantantimicrobialandleishmanicidalactivitiesandbiosecurity
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spelling ri-123456789-472442022-05-23T20:26:37Z Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança Tephrosia toxicaria (Sw.) Pers. extracts: prospection of anti-Aedes, antioxidant, antimicrobial and leishmanicidal activities, and biosecurity Silva, Giulian César da Uchoa, Adriana Ferreira https://orcid.org/0000-0002-4734-4228 http://lattes.cnpq.br/2134578884742038 https://orcid.org/0000-0002-3822-6502 http://lattes.cnpq.br/6644671747055211 Rocha, Hugo Alexandre de Oliveira https://orcid.org/0000-0003-2252-1221 http://lattes.cnpq.br/4651814546820796 Scortecci, Katia Castanho https://orcid.org/0000-0002-4690-2785 http://lattes.cnpq.br/4808910380593455 Ximenes, Maria de Fátima Freire de Melo http://lattes.cnpq.br/4231925509338101 Paiva, Patrícia Maria Guedes Morais, Selene Maia de Recursos naturais Extratos botânicos Timbó Arboviroses Estresse oxidativo Tephrosia toxicaria (Sw.) Pers. is a legume occurring in the Northeast Brazil that has attracted the attention of researchers because it serves as a matrix to obtain numerous adjuvant formulations for integrated management strategies of vectors and diseases, such as plant extracts, due to its selective toxicity and environmental compatibility. The search for new natural adjuvant formulations for the management of diseases, vectors and pathogens shows that plant extracts are still promising, especially considering the great Brazilian biodiversity, they are obtained from renewable sources, their toxic selectivity to target organisms and environmental compatibility. The development of the present research was stimulated by the possibility of expanding the repertoire of options for natural adjuvants, which aimed to investigate the bioactive potential and biosecurity of aqueous and hydroethanolic extracts from seeds, roots, stems and leaves of T. toxicaria. The first chapter of this thesis deals with a bibliographic review of the current state of art on the use of botanical insecticides in the integrated management of arbovirus vectors, with emphasis on species belonging to the genera Aedes, Culex and Anopheles. The second chapter describes the versatility of T. toxicaria extracts in the Aedes control, highlighting the hydroethanolic root extract (RHA) as the most promising larvicidal extract, demonstrating the lowest lethal concentration for 50% (300 µg/mL) and 90% (840 µg/mL) of Aedes larvae, observed at 24 h post-exposure. In field tests, RHA (840 µg/mL) is a promising oviposition deterrent agent, reducing Aedes egg laying by approximately 90%. In addition to manifesting insecticidal properties, RHA manifests antioxidant activity, mainly in control of metal ion homeostasis and restoration of the metabolic activity of cells under oxidative stress conditions, under low concentrations (100 µg/mL). Although leishmanicidal molecules are also expressed in RHA, its solubilization was less pronounced, requiring a higher concentration of extract to inhibit the growth of Leishmania amazonensis promastigotes (IC50 = 3.53 mg/mL). In the concentration range of 1024 – 32 μg/mL, RHA was not able to inhibit the growth of the bacterial and fungal strains investigated. RHA concentrates carbohydrates, phenolic compounds and proteins, with estimated molecular masses between 10 and 24 kDa. Protein analyzes suggest the expression of protease inhibitors and lectins in RHA. In the concentration range of 300 – 1 μg/mL, RHA does not compromise the initial development and biomass of Lactuca sativa; and in the concentration range of 1000 – 1 μg/mL, RHA does not compromise the metabolic activity of HepG2 (CC50 = 2.68 mg/mL) and 3T3 (CC50 = 5.07 mg/mL). The promising bioinsecticidal and antioxidant effects, associated with the absence of toxicity events against non-target organisms and cell lines, suggest T. toxicaria as a natural source to be sustainably exploited to obtain extracts with wide biological and pharmacological use. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq Tephrosia toxicaria (Sw.) Pers. é uma leguminosa ocorrente na região Nordeste do Brasil que vem despertando atenção de pesquisadores ao servir como matriz para obtenção de inúmeras formulações adjuvantes às estratégias de manejo integrado de vetores e doenças, como extratos vegetais, devido a sua toxicidade seletiva e compatibilidade ambiental. A possibilidade de ampliar o repertório de opções de adjuvantes naturais estimulou o desenvolvimento da presente pesquisa, que teve por objetivo investigar o potencial bioativo e biossegurança de extratos aquosos e hidroetanólicos de sementes, raízes, caules e folhas de T. toxicaria. O primeiro capítulo desta tese trata de uma revisão bibliográfica do estado da arte atual sobre a utilização de inseticidas botânicos adjuvantes ao manejo integrado de vetores de arbovírus, com ênfase em espécies dos gêneros Aedes, Culex e Anopheles. O segundo capítulo da tese descreve a versatilidade dos extratos de T. toxicaria no controle de Aedes, destacando o extrato hidroetanólico de raízes (RHA) como o extrato larvicida mais promissor, demonstrando as menores concentrações letais para 50% (300 µg/mL) e 90% (840 µg/mL) das larvas de Aedes, observadas 24 h pós-exposição. Em testes de campo, RHA (840 µg/mL) comportou-se como um agente deterrente de oviposição, reduzindo a postura de ovos de Aedes em aproximadamente 90%. Além de manifestar propriedades inseticidas, RHA apresenta atividade antioxidante, principalmente no controle da homeostase de íons metálicos e restauração da atividade metabólica de células em condições de estresse oxidativo, sob baixas concentrações (100 µg/mL). Embora moléculas leishmanicidas também sejam expressas em RHA, sua solubilização foi menos acentuada, demandando uma concentração maior de extrato para inibir o crescimento de formas promastigotas de Leishmania amazonensis (IC50 = 3,53 mg/mL). No intervalo de concentrações de 1024 – 32 μg/mL, RHA não foi capaz de inibir o crescimento das cepas bacterianas e fúngicas investigadas. RHA concentra carboidratos, compostos fenólicos e proteínas, com massas moleculares estimadas entre 10 e 24 kDa. Análises proteicas sugerem a expressão de inibidores de proteases e lectinas em RHA. No intervalo de concentrações de 300 – 1 μg/mL, RHA não compromete o desenvolvimento inicial e biomassa de Lactuca sativa; e no intervalo de concentrações de 1000 – 1 μg/mL, RHA não compromete a atividade metabólica de HepG2 (CC50 = 2,68 mg/mL) e 3T3 (CC50 = 5,07 mg/mL). Os promissores efeitos bioinseticida e antioxidante, associados a ausência de eventos de toxicidade contra organismos não-alvo e linhagens celulares, sugerem T. toxicaria como um recurso natural a ser sustentavelmente empregado na obtenção de extratos com amplo uso biológico e farmacológico. 2022-05-23T20:25:57Z 2022-05-23T20:25:57Z 2022-03-25 doctoralThesis SILVA, Giulian César da. Extratos de Tephrosia toxicaria (Sw.) Pers.: prospecção de atividades Anti-Aedes, antioxidante, antimicrobiana e leishmanicida, e biossegurança. 2022. 156f. Tese (Doutorado em Bioquímica e Biologia Molecular) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2022. https://repositorio.ufrn.br/handle/123456789/47244 pt_BR Acesso Aberto application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM BIOQUÍMICA E BIOLOGIA MOLECULAR

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