Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans

Escherichia coli and Candida albicans are responsible for harming both human and animal health. The most used method for the treatment of these diseases is the use of antibiotics, but the excessive use causes resistance of pathogenic microorganisms. The use of pyroligneous extract has been a widely...

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Autor principal: Lima, Nayane da Silva
Outros Autores: Pimenta, Alexandre Santos
Formato: bachelorThesis
Idioma:pt_BR
Publicado em: Universidade Federal do Rio Grande do Norte
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Endereço do item:https://repositorio.ufrn.br/handle/123456789/46352
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id ri-123456789-46352
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language pt_BR
topic Infecções
Carbonização da madeira
Maltodextrina
Infections
Wood carbonization
Maltodextrin
spellingShingle Infecções
Carbonização da madeira
Maltodextrina
Infections
Wood carbonization
Maltodextrin
Lima, Nayane da Silva
Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans
description Escherichia coli and Candida albicans are responsible for harming both human and animal health. The most used method for the treatment of these diseases is the use of antibiotics, but the excessive use causes resistance of pathogenic microorganisms. The use of pyroligneous extract has been a widely used alternative. In order to facilitate the transport, handling and application, the use of a new formulation for the EP, in gel and solid, is possible from the addition of maltodextrin to the solution, in order to soften the odor and provide the viscosity range for the new products. formulas. The behavior and bactericidal and fungicidal potential of pyroligneous extract in gel and solid on Escherichia coli and Candida albicans were aimed at, in addition to establishing the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) and Minimum Fungicide Concentration (MFC). To establish the concentrations to be used in the microdilution test, the well disk agar diffusion test was performed in advance. For the microdilution test the concentrations used were: For E. coli with the EP in gel it was used: 15%, 5%, 1%, 0.5%, 0.25% and 0.125%; and for solid EP: 60, 50, 40, 30, 20, 18, 16, 10 and 1 mg. For C. albicans with the EP in gel was used: 4%, 3%, 2%, 1%, 0.5% and 0.25%; and for solid EP: 18, 16, 14, 12, 10, 8, 6, 4 mg. The concentrations were diluted and the microdilution test was mounted on two plates, one for the bacteria and one for the fungus. The concentrations were added, followed by their respective pathogens. The positive control treatment was performed with the addition of the culture medium corresponding to each one of them and the pathogen, while the negative control was used with the antibiotics gentamicin and fluconazole. After the incubation time, the Plates were analyzed in the Microplate Reader and the results submitted to one-way ANOVA, followed by the Tukey Test. The EP in gel on E. coli, obtained the same results for MIC and MBC: 1 ml, value that was the lowest non-significant concentration compared to the other concentrations. The MIC and MBC of solid EP for E. coli were 10 mg/m and 16 mg/ml, respectively. For the EP in gel on C. albicans, the MIC and MFC obtained equal values, 1 ml. For solid EP, MIC and MFC also showed the same results: 18 mg/ml for both. Therefore, it was possible to prove the antibacterial and antifungal action of the pyroligneous extract in gel and solid, in the respective concentrations, on Escherichia Coli and Candida albicans.
author2 Pimenta, Alexandre Santos
author_facet Pimenta, Alexandre Santos
Lima, Nayane da Silva
format bachelorThesis
author Lima, Nayane da Silva
author_sort Lima, Nayane da Silva
title Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans
title_short Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans
title_full Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans
title_fullStr Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans
title_full_unstemmed Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans
title_sort potencial bactericida e fungicida do extrato pirolenhoso de eucalyptus urograndis nas formulações em gel e sólido sobre escherichia coli e candida albicans
publisher Universidade Federal do Rio Grande do Norte
publishDate 2022
url https://repositorio.ufrn.br/handle/123456789/46352
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AT limanayanedasilva bactericidalandfungicidalpotentialofpyrolenyextractofeucalyptusurograndisingelandsolidformulationsonescherichiacoliandcandidaalbicans
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spelling ri-123456789-463522022-09-09T12:53:41Z Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans Bactericidal and fungicidal potential of pyroleny extract of Eucalyptus urograndis in gel and solid formulations on Escherichia coli and Candida albicans Lima, Nayane da Silva Pimenta, Alexandre Santos https://orcid.org/0000-0001-9633-0749 http://lattes.cnpq.br/8157164488516750 https://orcid.org/0000-0002-2134-2080 http://lattes.cnpq.br/5285780811057236 Souza, Elias Costa de http://lattes.cnpq.br/2443233154986868 Carnaval, Tatiane Kelly Barbosa de Azevêdo http://lattes.cnpq.br/3960259666564003 Infecções Carbonização da madeira Maltodextrina Infections Wood carbonization Maltodextrin Escherichia coli and Candida albicans are responsible for harming both human and animal health. The most used method for the treatment of these diseases is the use of antibiotics, but the excessive use causes resistance of pathogenic microorganisms. The use of pyroligneous extract has been a widely used alternative. In order to facilitate the transport, handling and application, the use of a new formulation for the EP, in gel and solid, is possible from the addition of maltodextrin to the solution, in order to soften the odor and provide the viscosity range for the new products. formulas. The behavior and bactericidal and fungicidal potential of pyroligneous extract in gel and solid on Escherichia coli and Candida albicans were aimed at, in addition to establishing the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) and Minimum Fungicide Concentration (MFC). To establish the concentrations to be used in the microdilution test, the well disk agar diffusion test was performed in advance. For the microdilution test the concentrations used were: For E. coli with the EP in gel it was used: 15%, 5%, 1%, 0.5%, 0.25% and 0.125%; and for solid EP: 60, 50, 40, 30, 20, 18, 16, 10 and 1 mg. For C. albicans with the EP in gel was used: 4%, 3%, 2%, 1%, 0.5% and 0.25%; and for solid EP: 18, 16, 14, 12, 10, 8, 6, 4 mg. The concentrations were diluted and the microdilution test was mounted on two plates, one for the bacteria and one for the fungus. The concentrations were added, followed by their respective pathogens. The positive control treatment was performed with the addition of the culture medium corresponding to each one of them and the pathogen, while the negative control was used with the antibiotics gentamicin and fluconazole. After the incubation time, the Plates were analyzed in the Microplate Reader and the results submitted to one-way ANOVA, followed by the Tukey Test. The EP in gel on E. coli, obtained the same results for MIC and MBC: 1 ml, value that was the lowest non-significant concentration compared to the other concentrations. The MIC and MBC of solid EP for E. coli were 10 mg/m and 16 mg/ml, respectively. For the EP in gel on C. albicans, the MIC and MFC obtained equal values, 1 ml. For solid EP, MIC and MFC also showed the same results: 18 mg/ml for both. Therefore, it was possible to prove the antibacterial and antifungal action of the pyroligneous extract in gel and solid, in the respective concentrations, on Escherichia Coli and Candida albicans. A Escherichia coli e a Candida albicans são responsáveis por prejudicarem tanto à saúde humana quanto a animal. O método mais utilizado para o tratamento dessas enfermidades é a utilização de antibióticos, porém o uso demasiado causa resistência dos micro-organismos patogénicos. A utilização do extrato pirolenhoso vem sendo uma alternativa bastante empregada. Visando facilitar o transporte, manuseio e aplicação, a utilização de uma nova formulação para o EP, em gel e sólido, é possível a partir da adição de maltodextrina a solução, a fim de suavizar o odor e proporcionar o alcance da viscosidade para as novas fórmulas. Objetivou-se o comportamento e o potencial bactericida e fungicida extrato pirolenhoso em gel e sólido sobre a Escherichia coli e a Candida albicans, além de estabelecer a Concentração Inibitória Mínima (CIM) e a Concentração Bactericida Mínima (CBM) e Concentração Fungicida Mínima (CFM). Para estabelecer as concentrações a serem utilizadas no teste de microdiluição, foi o realizado o teste de difusão em ágar por disco de poço antecipadamente. Para o teste de microdiluição as concentrações utilizadas foram: Para a E. coli com o EP em gel utilizou-se: 15%, 5%, 1%, 0,5%, 0,25% e 0,125% e para o EP sólido: 60, 50, 40, 30, 20, 18, 16, 10 e 1 mg. Para a C. albicans com o EP em gel foi utilizado: 4%, 3%, 2%, 1%, 0,5% e 0,25%; e para o EP sólido: 18, 16, 14, 12, 10, 8, 6, 4 mg. As concentrações foram diluídas e o teste de microdiluição foi montado em duas placas, uma para a bactéria e uma para o fungo. Adicionou-se as concentrações, seguidas dos seus respectivos patógenos. O tratamento controle positivo foi realizado com a adição do meio de cultura correspondente a cada uma delas e o patógeno, já o controle negativo foi utilizado os antibióticos gentamicina e fluconazol. Após o tempo de incubação, as Placas foram analisadas no Leitor de Microplacas e os resultados submetidos a ANOVA um critério, seguido do Teste de Tukey. O EP em gel sobre a E. coli, obteve os resultados para CIM e CBM iguais: 1 ml, valor que foi a menor concentração não significativa comparado as outras concentrações. A CIM e CBM do EP sólido para a E. coli foi 10 mg/m e 16 mg/ml, respectivamente. Para o EP em gel sobre a C. albicans, a CIM e CFM obtiveram valores iguais, 1 ml. Para o EP sólido a CIM e a CFM também apresentaram resultados iguais: 18 mg/ml para ambas. Portanto, foi possível comprovar a ação antibactericida e antifúngica do extrato pirolenhoso em gel e sólido, nas respectivas concentrações, sobre a Escherichia Coli e a Candida albicans. 2022-02-25T18:54:04Z 2022-02-25T18:54:04Z 2022-02-09 bachelorThesis LIMA, Nayane da Silva. Potencial bactericida e fungicida do extrato pirolenhoso de Eucalyptus urograndis nas formulações em gel e sólido sobre Escherichia coli e Candida albicans. 2022. 51f. Trabalho de Conclusão de Curso (Graduação em Engenharia Florestal), Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do Norte, Macaíba, 2022. https://repositorio.ufrn.br/handle/123456789/46352 pt_BR Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN Engenharia Florestal Unidade Acadêmica Especializada em Ciências Agrárias