Nanopartículas híbridas lipídico-poliméricas contendo coenzima-Q10 para potencial atividade anticâncer
Cancer is a disease of high incidence and prevalence all over the planet and its treatment is quite problematic due to the high toxicity degree of the drugs used, difficulty in targeting and limitations related to evasive mechanisms of tumors. Coenzyme Q-10 is an endogenous antioxidant molecule,...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/46095 |
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| Resumo: | Cancer is a disease of high incidence and prevalence all over the planet and its
treatment is quite problematic due to the high toxicity degree of the drugs used,
difficulty in targeting and limitations related to evasive mechanisms of tumors.
Coenzyme Q-10 is an endogenous antioxidant molecule, highly lipophilic and widely
studied for several applications, including its potential anticancer effect.
Nanotechnology is used as a device to minimize these problems and be able to
promote safer, more effective and humanized treatment for patients. Within
nanotechnology, hybrid lipid-polymeric nanoparticles are new systems that combine
the versatility of polymers with the biocompatibility of lipids, producing systems that
bring out the best of both structuring agents. It was aimed to obtain and characterize
NPH of PLGA and cholesterol loaded with CoQ10. In this work, was caried out the
incorporation of Coenzyme Q-10 into lipid-polymeric cholesterol/PLGA hybrid
nanoparticles, stabilized with Span 80® and Tween 80®, by the nanoprecipitation
method. The characterization of these nanoparticles was evaluated in terms of
diameter, polydispersity index (PdI) zeta potential (PZ) and pH, and the same
parameters were used to measure the system stability during 45 days. The of
Coenzyme Q-10 encapsulation efficiency was measured by an indirect method, by
VIVASPIN® filters with 10 KDa cut-off, and a hemolysis test was performed to assess
the system's compatibility with erythrocytes. Nanoparticles were obtained with a size
between 100-200 nm, with low PdI (around 0.1), the PZ showed that the nanoparticles
produced are anionic with a surface charge around 18 mV, with encapsulation
efficiency above 90% and a high degree of hemolysis was observed as a preliminary
result. The systems were successfully obtained and characterized, allowing progress
in the study, seeking improvements and new tests. |
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