Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil

Oral mucositis (OM) is characterized by the presence of severe ulcers in the oral region that affects patients treated with chemotherapy. It occurs in almost all patients who receive radiotherapy of the head and neck, as well as patients who undergo hematopoietic cell transplantation. The pathophysi...

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Principais autores: Ribeiro, Susana Barbosa, Araújo, Aurigena Antunes de, Oliveira, Maisie Mitchele Barbosa, Silva, Alaine Maria dos Santos, Silva Júnior, Arnóbio Antônio da, Guerra, Gerlane Coelho Bernardo, Brito, Gerly Anne de Castro, Leitão, Renata Ferreira de Carvalho, Araújo Júnior, Raimundo Fernandes de, Garcia, Vinícius Barreto, Vasconcelos, Roseane Carvalho, Medeiros, Caroline Addison Carvalho Xavier de
Formato: article
Idioma:English
Publicado em: MDPI
Assuntos:
5-fluorouracil
PLGA
Nanoparticles
Oral mucositis
Endereço do item:https://repositorio.ufrn.br/handle/123456789/46007
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spelling ri-123456789-460072022-04-08T11:56:22Z Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil Ribeiro, Susana Barbosa Araújo, Aurigena Antunes de Oliveira, Maisie Mitchele Barbosa Silva, Alaine Maria dos Santos Silva Júnior, Arnóbio Antônio da Guerra, Gerlane Coelho Bernardo Brito, Gerly Anne de Castro Leitão, Renata Ferreira de Carvalho Araújo Júnior, Raimundo Fernandes de Garcia, Vinícius Barreto Vasconcelos, Roseane Carvalho Medeiros, Caroline Addison Carvalho Xavier de 5-fluorouracil PLGA Nanoparticles Oral mucositis Oral mucositis (OM) is characterized by the presence of severe ulcers in the oral region that affects patients treated with chemotherapy. It occurs in almost all patients who receive radiotherapy of the head and neck, as well as patients who undergo hematopoietic cell transplantation. The pathophysiology of OM is complex, and there is no effective therapy. The aim of this study was to evaluate the effect of dexamethasone-loaded poly(D,L-Lactic-co-glycolic) nanoparticles (PLGA-DEX NPs) on an OM model induced in hamsters. The NPs were synthesized using the emulsification-solvent evaporation method and were characterized by the size, zeta potential, encapsulation efficiency, atomic force microscopy, physicochemical stability, and the in vitro release. The OM was induced by the administration of 5-FU on the first and second days and mechanical trauma on the 4th day of the experiment. PLGA-DEX NPs were administered to treat OM. The animals were euthanized on the 10th day. Macroscopic and histopathological analyses were performed, measurement of malonaldehyde (MDA) and ELISA was used to determine the levels of IL-1β and TNF-α. Immunoexpressions of NF-κB, COX-2, and TGF-β were determined by immunohistochemistry, and qRT-PCR was used to quantify the gene expression of the GILZ, MKP1, and NF-κB p65. The PLGA-DEX NPs (0.1 mg/kg) significantly reduced macroscopic and histopathological scores, decreased MDA, TNF-α and IL-1β levels, immunostaining for NF-κB, COX-2, TGF-β, and suppressed NF-κB p65 mRNA expression, but increased GILZ and MKP1 expression 2022-02-16T16:32:59Z 2022-02-16T16:32:59Z 2021-01-04 article RIBEIRO, Susana Barbosa et al. Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil. Pharmaceutics, [s. l.], v. 13, n. 1, p. 53, 4 jan. 2021. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823510/. Acesso em: 16 fev. 2022. http://dx.doi.org/10.3390/pharmaceutics13010053 1999-4923 https://repositorio.ufrn.br/handle/123456789/46007 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf MDPI
institution Repositório Institucional
collection RI - UFRN
language English
topic 5-fluorouracil
PLGA
Nanoparticles
Oral mucositis
spellingShingle 5-fluorouracil
PLGA
Nanoparticles
Oral mucositis
Ribeiro, Susana Barbosa
Araújo, Aurigena Antunes de
Oliveira, Maisie Mitchele Barbosa
Silva, Alaine Maria dos Santos
Silva Júnior, Arnóbio Antônio da
Guerra, Gerlane Coelho Bernardo
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira de Carvalho
Araújo Júnior, Raimundo Fernandes de
Garcia, Vinícius Barreto
Vasconcelos, Roseane Carvalho
Medeiros, Caroline Addison Carvalho Xavier de
Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil
description Oral mucositis (OM) is characterized by the presence of severe ulcers in the oral region that affects patients treated with chemotherapy. It occurs in almost all patients who receive radiotherapy of the head and neck, as well as patients who undergo hematopoietic cell transplantation. The pathophysiology of OM is complex, and there is no effective therapy. The aim of this study was to evaluate the effect of dexamethasone-loaded poly(D,L-Lactic-co-glycolic) nanoparticles (PLGA-DEX NPs) on an OM model induced in hamsters. The NPs were synthesized using the emulsification-solvent evaporation method and were characterized by the size, zeta potential, encapsulation efficiency, atomic force microscopy, physicochemical stability, and the in vitro release. The OM was induced by the administration of 5-FU on the first and second days and mechanical trauma on the 4th day of the experiment. PLGA-DEX NPs were administered to treat OM. The animals were euthanized on the 10th day. Macroscopic and histopathological analyses were performed, measurement of malonaldehyde (MDA) and ELISA was used to determine the levels of IL-1β and TNF-α. Immunoexpressions of NF-κB, COX-2, and TGF-β were determined by immunohistochemistry, and qRT-PCR was used to quantify the gene expression of the GILZ, MKP1, and NF-κB p65. The PLGA-DEX NPs (0.1 mg/kg) significantly reduced macroscopic and histopathological scores, decreased MDA, TNF-α and IL-1β levels, immunostaining for NF-κB, COX-2, TGF-β, and suppressed NF-κB p65 mRNA expression, but increased GILZ and MKP1 expression
format article
author Ribeiro, Susana Barbosa
Araújo, Aurigena Antunes de
Oliveira, Maisie Mitchele Barbosa
Silva, Alaine Maria dos Santos
Silva Júnior, Arnóbio Antônio da
Guerra, Gerlane Coelho Bernardo
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira de Carvalho
Araújo Júnior, Raimundo Fernandes de
Garcia, Vinícius Barreto
Vasconcelos, Roseane Carvalho
Medeiros, Caroline Addison Carvalho Xavier de
author_facet Ribeiro, Susana Barbosa
Araújo, Aurigena Antunes de
Oliveira, Maisie Mitchele Barbosa
Silva, Alaine Maria dos Santos
Silva Júnior, Arnóbio Antônio da
Guerra, Gerlane Coelho Bernardo
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira de Carvalho
Araújo Júnior, Raimundo Fernandes de
Garcia, Vinícius Barreto
Vasconcelos, Roseane Carvalho
Medeiros, Caroline Addison Carvalho Xavier de
author_sort Ribeiro, Susana Barbosa
title Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil
title_short Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil
title_full Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil
title_fullStr Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil
title_full_unstemmed Effect of Dexamethasone-Loaded PLGA Nanoparticles on Oral Mucositis Induced by 5-Fluorouracil
title_sort effect of dexamethasone-loaded plga nanoparticles on oral mucositis induced by 5-fluorouracil
publisher MDPI
publishDate 2022
url https://repositorio.ufrn.br/handle/123456789/46007
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