Eficácia anti-Hipergliceminante, anti perda óssea e anti-inflamatória do probiótico Lactobacillus rhamnosus EM 1107 em modelo experimental de periodontite associada a diabetes
Periodontal disease (PD) and diabetes mellitus (DM) have a well-established bidirectional relationship in the literature as they share similar chronic inflammatory mechanisms. Due to its microbial and immunological effects, probiotic therapy has been suggested as an adjuvant to non-surgical periodon...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/45708 |
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| Resumo: | Periodontal disease (PD) and diabetes mellitus (DM) have a well-established bidirectional
relationship in the literature as they share similar chronic inflammatory mechanisms. Due
to its microbial and immunological effects, probiotic therapy has been suggested as an
adjuvant to non-surgical periodontal treatment. Considering metformin (MET) as the
standard drug for the treatment of diabetes, the aim of this study was to evaluate the antihyperglycemic, anti-bone loss and anti-inflammatory efficacy of the probiotic
Lactobacillus rhamnosus EM1107 in an experimental model of ligature-induced
periodontitis in diabetic rats treated with metformin. This pre-clinical, in vivo,
randomized, blinded and controlled trial consisted of 114 male Wistar rats randomly
divided into six groups of 19 animals each: control, ligation, ligation/DM, ligation/Prob,
ligation/DM/Prob and ligation /DM/Prob/Met. For 30 days the animals received a
probiotic via oral gavage. DM was induced on the 14th day of the experiment with
injection of Streptozotocin (STZ) into the penile vein, followed by ligation for PD
induction and MET gavage on the 19th day, and euthanasia on the 30th day. An extremely
significant reduction in serum glucose was observed in both the Prob and Metformin
groups (p<0.001) by biochemical analysis. Microtomographic analysis (μCT) in the
maxilla showed that diabetic animals treated with probiotic and metformin had
significantly lower linear and volumetric bone loss than those that had no treatment at all.
By histomorphological analysis (HE), all groups that received probiotics had a reduction
in inflammatory infiltrate, in addition to preservation of cementum and alveolar bone.
The TRAP analysis revealed that the Ligature/DM/Prob/Met group had the greatest
reduction in osteoclast count (p<0.01). There was a significant reduction in IL-1β and
TNF-α in the Ligature/Prob (p<0.05) and Ligature/DM/Prob (p<0.01) groups by means
of the ELISA assay in gingival tissue. Our findings suggest the anti-hyperglycemic, antibone loss and anti-inflammatory efficacy of the probiotic Lactobacillus rhamnosus
EM1107 administered either alone or in association with Metformin in DM with PD. |
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