Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography

−A phenomenological model was used to describe sequentially the three steps (flowthrough, washing and elution) of expanded bed adsorption chromatography for recovery of chitosanases from Bacillus cereus. Additionally, a hybrid strategy for model parameter estimation was carried out using particle...

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প্রধান লেখক: Souza, Domingos Fabiano de Santana, Padilha, Carlos Eduardo de Araújo, Araújo, Nathália Kelly de, Oliveira, Jackson Araújo de, Macedo, Gorete Ribeiro de, Santos, Everaldo Silvino dos
বিন্যাস: article
ভাষা:English
প্রকাশিত: Springer
বিষয়গুলি:
Modeling
Expanded bed adsorption
Chitosanases
Particle swarm optimization
Gauss-Newton
Optimization
অনলাইন ব্যবহার করুন:https://repositorio.ufrn.br/handle/123456789/45193
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spelling ri-123456789-451932023-02-14T19:50:55Z Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography Souza, Domingos Fabiano de Santana Padilha, Carlos Eduardo de Araújo Araújo, Nathália Kelly de Oliveira, Jackson Araújo de Macedo, Gorete Ribeiro de Santos, Everaldo Silvino dos Modeling Expanded bed adsorption Chitosanases Particle swarm optimization Gauss-Newton Optimization −A phenomenological model was used to describe sequentially the three steps (flowthrough, washing and elution) of expanded bed adsorption chromatography for recovery of chitosanases from Bacillus cereus. Additionally, a hybrid strategy for model parameter estimation was carried out using particle swarm optimization and Gauss-Newton algorithms. The model was validated with independent experimental data and the statistical criteria (χ2 and mean squared error tests) showed that the hybrid strategy was more promising than just the heuristic method. With the calibrated model, surface response methodology was applied to obtain the optimal operational conditions, and experiments were performed to confirm these results. Overall, a value of 41.08% for yield was obtained using 700 mM NaCl during elution. In summary, all approach employed in this work was relevant for maximizing the yield of the chromatographic process 2021-12-06T18:24:29Z 2021-12-06T18:24:29Z 2016-07 article PADILHA, Carlos Eduardo de Araújo; ARAðJO, Nathália Kelly de; SOUZA, Domingos Fabiano de Santana; OLIVEIRA, Jackson Araújo de; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography. Korean Journal Of Chemical Engineering, [S.L.], v. 33, n. 9, p. 2650-2658, 18 jul. 2016. Springer Science and Business Media LLC. http://dx.doi.org/10.1007/s11814-016-0127-1. Disponível em: https://link.springer.com/article/10.1007/s11814-016-0127-1. Acesso em: 05 nov. 2021. 1975-7220 https://repositorio.ufrn.br/handle/123456789/45193 10.1007/s11814-016-0127-1. en application/pdf Springer
institution Repositório Institucional
collection RI - UFRN
language English
topic Modeling
Expanded bed adsorption
Chitosanases
Particle swarm optimization
Gauss-Newton
Optimization
spellingShingle Modeling
Expanded bed adsorption
Chitosanases
Particle swarm optimization
Gauss-Newton
Optimization
Souza, Domingos Fabiano de Santana
Padilha, Carlos Eduardo de Araújo
Araújo, Nathália Kelly de
Oliveira, Jackson Araújo de
Macedo, Gorete Ribeiro de
Santos, Everaldo Silvino dos
Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography
description −A phenomenological model was used to describe sequentially the three steps (flowthrough, washing and elution) of expanded bed adsorption chromatography for recovery of chitosanases from Bacillus cereus. Additionally, a hybrid strategy for model parameter estimation was carried out using particle swarm optimization and Gauss-Newton algorithms. The model was validated with independent experimental data and the statistical criteria (χ2 and mean squared error tests) showed that the hybrid strategy was more promising than just the heuristic method. With the calibrated model, surface response methodology was applied to obtain the optimal operational conditions, and experiments were performed to confirm these results. Overall, a value of 41.08% for yield was obtained using 700 mM NaCl during elution. In summary, all approach employed in this work was relevant for maximizing the yield of the chromatographic process
format article
author Souza, Domingos Fabiano de Santana
Padilha, Carlos Eduardo de Araújo
Araújo, Nathália Kelly de
Oliveira, Jackson Araújo de
Macedo, Gorete Ribeiro de
Santos, Everaldo Silvino dos
author_facet Souza, Domingos Fabiano de Santana
Padilha, Carlos Eduardo de Araújo
Araújo, Nathália Kelly de
Oliveira, Jackson Araújo de
Macedo, Gorete Ribeiro de
Santos, Everaldo Silvino dos
author_sort Souza, Domingos Fabiano de Santana
title Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography
title_short Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography
title_full Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography
title_fullStr Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography
title_full_unstemmed Modeling and simulation of Bacillus cereus chitosanase activity during purification using expanded bed chromatography
title_sort modeling and simulation of bacillus cereus chitosanase activity during purification using expanded bed chromatography
publisher Springer
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/45193
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