Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were the lipases A and B from Candida antarctica, the lipases from Rhizomocur miehei and, Them...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Principais autores: Peña, Sara Arana, Rios, Nathalia Saraiva, Sanchez, Carmen Mendez, Lokha, Yuliya, Carballares, Diego, Gonçalves, Luciana Rocha Barros, Lafuente, Roberto Fernandez
Formato: article
Idioma:English
Publicado em: Elsevier
Assuntos:
Enzyme coimmobilization
Enzyme ordering
Combilipases
Endereço do item:https://repositorio.ufrn.br/handle/123456789/45068
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id ri-123456789-45068
record_format dspace
spelling ri-123456789-450682024-03-19T04:02:00Z Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering Peña, Sara Arana Rios, Nathalia Saraiva Sanchez, Carmen Mendez Lokha, Yuliya Carballares, Diego Gonçalves, Luciana Rocha Barros Lafuente, Roberto Fernandez Enzyme coimmobilization Enzyme ordering Combilipases This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were the lipases A and B from Candida antarctica, the lipases from Rhizomocur miehei and, Themomyces lanuginosus and the phospholipase Lecitase Ultra. The utilized strategy was a layer by layer immobilization, coating the immobilized enzymes with polyethylenimine followed by the crosslinking of the enzyme and PEI with glutaraldehyde to prevent enzyme release, and them adding a new lipase layer. The use of previously inactivated biocatalysts (using diethyl p-nitrophenylphosphate) permitted to visualize the immobilization of each enzyme layer, which was later confirmed by SDS-PAGE. This also confirmed the successful and complete covalent crosslinking of the glutaraldehyde treated enzyme layers. Activity of the combibiocatalysts was followed using diverse substrates. The protocol was successful and permitted to immobilize in an ordered way the 5 different enzymes in a down-up distribution 2030-12 2021-11-29T21:16:03Z 2020-02-15 article ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087. 0141-8130 https://repositorio.ufrn.br/handle/123456789/45068 10.1016/j.ijbiomac.2019.10.087 en Elsevier
institution Repositório Institucional
collection RI - UFRN
language English
topic Enzyme coimmobilization
Enzyme ordering
Combilipases
spellingShingle Enzyme coimmobilization
Enzyme ordering
Combilipases
Peña, Sara Arana
Rios, Nathalia Saraiva
Sanchez, Carmen Mendez
Lokha, Yuliya
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
description This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were the lipases A and B from Candida antarctica, the lipases from Rhizomocur miehei and, Themomyces lanuginosus and the phospholipase Lecitase Ultra. The utilized strategy was a layer by layer immobilization, coating the immobilized enzymes with polyethylenimine followed by the crosslinking of the enzyme and PEI with glutaraldehyde to prevent enzyme release, and them adding a new lipase layer. The use of previously inactivated biocatalysts (using diethyl p-nitrophenylphosphate) permitted to visualize the immobilization of each enzyme layer, which was later confirmed by SDS-PAGE. This also confirmed the successful and complete covalent crosslinking of the glutaraldehyde treated enzyme layers. Activity of the combibiocatalysts was followed using diverse substrates. The protocol was successful and permitted to immobilize in an ordered way the 5 different enzymes in a down-up distribution
format article
author Peña, Sara Arana
Rios, Nathalia Saraiva
Sanchez, Carmen Mendez
Lokha, Yuliya
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
author_facet Peña, Sara Arana
Rios, Nathalia Saraiva
Sanchez, Carmen Mendez
Lokha, Yuliya
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
author_sort Peña, Sara Arana
title Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_short Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_full Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_fullStr Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_full_unstemmed Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_sort coimmobilization of different lipases: simple layer by layer enzyme spatial ordering
publisher Elsevier
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/45068
work_keys_str_mv AT penasaraarana coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
AT riosnathaliasaraiva coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
AT sanchezcarmenmendez coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
AT lokhayuliya coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
AT carballaresdiego coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
AT goncalveslucianarochabarros coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
AT lafuenterobertofernandez coimmobilizationofdifferentlipasessimplelayerbylayerenzymespatialordering
_version_ 1797696436844888064

Registros relacionados