Quantificação de biomoléculas e investigação de atividades biológicas do extrato e frações do cogumelo Clavulina coralloides
Currently, the search for natural sources that have biomolecules with biomedical applicability is widely performed and seen as promising. The use of fungi as a source of compounds with importance in biology and medicine is growing and prosperous, in view of the relevance of studies that demonstrate...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/43236 |
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| Resumo: | Currently, the search for natural sources that have biomolecules with biomedical applicability is widely performed and seen as promising. The use of fungi as a source of compounds with importance in biology and medicine is growing and prosperous, in view of the relevance of studies that demonstrate success as antimicrobial, anticancer agents, anticoagulants, antioxidants, immunopotentiators, among others. But there are many fungi with possible biotechnological potential that remain unexplored. Therefore, this unprecedented work aims to present the characterization of the crude extract (CE) and fractions from a fractionation using increasing concentrations of ammonium sulfate (F1, F2 and F3) of the fungus Clavulina coralloides, exploring its hemagglutinating, antioxidant (TAC), anticoagulant (APTT and PT), protease inhibitor and cytotoxic (MTT) activities. The crude extract and its fractions had their proteins, carbohydrates and total phenolic compounds quantified. The samples also had their protein profiles analyzed by SDS-PAGE. All fractions exhibited haemagglutinating activity, although F2 stood out for this and for the higher protein content. CE was the only one with determinable quantification of carbohydrate, and higher concentration of phenolic compounds. All samples showed positive results for the inhibition of proteases, however they did not exhibit considerable antioxidant activity. No sample showed cytotoxic activity for normal cultured fibroblasts (3T3), on the contrary, they promoted induction of their proliferation in all tested concentrations. The data obtained require the need for more in-depth tests, together with the investigation of possible new bioactive molecules. |
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