Efeito crônico da varfarina na biodistibuição do 99mTc-DTPA em ratos Wistar
Oral anticoagulant therapy, such as warfarin, is performed in the prophylaxis of thrombosis and embolism in many vascular disorders that affect mostly individuals with more advanced age. It acts by inhibiting the formation of coagulation factors II, VII, IX and X, and therefore, an antagonist of vit...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/43153 |
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| Resumo: | Oral anticoagulant therapy, such as warfarin, is performed in the prophylaxis of thrombosis and embolism in many vascular disorders that affect mostly individuals with more advanced age. It acts by inhibiting the formation of coagulation factors II, VII, IX and X, and therefore, an antagonist of vitamin K. In turn, drugs labeled with radioactive isotopes are commonly used in nuclear medicine for diagnostic purposes. As an example, there is technetium-99m, which is widely used radioisotope in human scans for diagnosis of various diseases and organ dysfunction. It has ideal physical characteristics for use in diagnostic nuclear medicine, such as is mono-gamma-emitting low energy (140keV), has a relatively short half-life (6.02 h) and emits no particulate radiation (α or β -). This study aimed to evaluate if the warfarin chronically administered for 10 consecutive days, alters the normal biodistribution of the radiopharmaceutical acid diethylene triamine pentaacetic labeled with technetium-99m (99mTc-DTPA) in the kidneys, bladder and blood of Wistar rat. For this, we used 12 Wistar rats, 3 months old and body weight ± 300 g, they were divided into control group (n = 6) and treated group (n = 6). The control group received 0.25 ml of filtered water and the treated group received 0.25 ml of warfarin solution (0,30mg/kg), both by gavage during 10 days. At the 10th day it was administered 0.1 ml of 99mTc-DTPA (0,66MBq radioactivity) via tail vein 1 hour after the treatment of two groups. After 1 h, all the animals were euthanasia for removal of the kidneys, bladder and blood sample. These samples were brought to the gamma counter to determine the percentage of the total injected activity per gram tissue (% ATI/g). There was a statistically significant increase in the % ATI/g of the kidney, respectively in the treated group compared to the control group (1.732±0.145 and 0.802±0.170) and a decrease in bladder (0.646±0,131 and 2.786±0.481 e) and blood (0.050±0.009 and 1.226±0.105) of treated group compared to control group, respectively. Based on these results, it appears that warfarin altered the normal 99mTc-DTPA biodistribution, probably, due to their biological and/or metabolites effects. |
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