Análises histopatológicas do fígado da prole de camundongos (Mus musculus) submetidos ao etanol durante os períodos pré e pós natal
The initial effects of ethanol consumption can include a feeling of pleasure and well-being, however, there is no safe concentration that can be ingested without harm or health complications. When it comes to the female body, the use of this drug takes longer to be excreted, as the rate of metaboliz...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/39571 |
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| Resumo: | The initial effects of ethanol consumption can include a feeling of pleasure and well-being, however, there is no safe concentration that can be ingested without harm or health complications. When it comes to the female body, the use of this drug takes longer to be excreted, as the rate of metabolization is slower, making its effects more prolonged. During pregnancy, ethanol has the ability to cross both the placental barrier and be present in breast milk and, as it has a hepatotoxic character, it can trigger a series of negative effects that can harm the health of the offspring, cause complications in childbirth or even death fetal. Histopathological studies showed that the liver of newborns exposed to ethanol during the intrauterine period resembled the alterations found in the livers of adult alcohol users, concluding that excessive and prolonged exposure can affect the structure and function of the liver, causing alterations present in Alcoholic Liver Disease (ALD). The aim of this study was to analyze the liver morphology and histopathology of the offspring of mice exposed to alcohol during the periods of gestation, lactation and gestation plus lactation. After approval by the Animal Use Ethics Committee, 23 mice of the Mus musculus species were used, divided into 4 groups: Control Group (CG) n=6: pregnant women who received water and food ad libitum during pregnancy and lactation; Pregnancy Ethanol Group (GETG) n=5: pregnant women who received ethanol (15%) from copulation to birth; Ethanol Lactation Group (GETL) n=6: pregnant women who received ethanol (15%) from the day the pups were born until weaning; Ethanol Group Pregnancy Lactation (GETGL) n=6: pregnant women received ethanol from copulation until weaning. The puppies were weaned and weighed on the 21st day, after the 34th and 36th day of life, the animals were weighed again and submitted to the euthanasia protocol for the removal and conservation of the liver for further macroscopic analysis and preparation of slides. The data obtained were analyzed by ANOVA and GABRIEL post hoc, with a significance level of 5% (P>0.05). The results showed a reduction in body weight after weaning of animals in groups GETL and GETGL, when compared to group GETG, there was also a decrease in final body weight and liver weight in animals in group GETL when compared to GETG. Exposure to alcohol during the lactation phase may have influenced the composition, aroma and production of milk, negatively inferring the nutrition of puppies. Semiquantitative analyzes showed that the GETGL group presented a greater sum of liver damage when compared to the other groups. In the histopathological alterations, all experimental groups showed tissue damage characteristic of phases of DHA such as: micro and macro vesicular steatosis, ballooning degeneration, inflammatory infiltrate and areas suggestive of necrosis. These findings can be explained by the toxic character of ethanol resulting from its metabolism, which gives the liver tissue an accumulation of reactive oxygen species, affecting both cell homeostasis and providing a pro-inflammatory environment. Thus, in the animal model used, acute alcohol exposure during pregnancy, lactation and pregnancy plus lactation probably had hepatotoxic effects on the offspring. |
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