Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal.
Using of plants, extracts, and isolated metabolites is becoming very popular nowadays. Natural medicine does not acts only preventively, as also treats many diseases caused by resistant microorganisms. This study aimed to evaluate the antimicrobial action of the vegetable extract of Lycium barbarum...
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Fitoterapia Microbiologia e Cromatografia em camada delgada. |
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Fitoterapia Microbiologia e Cromatografia em camada delgada. Carvalho, Isabelle Helena Gurgel de Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
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Using of plants, extracts, and isolated metabolites is becoming very popular nowadays. Natural medicine does not acts only preventively, as also treats many diseases caused by resistant microorganisms. This study aimed to evaluate the antimicrobial action of the vegetable extract of Lycium barbarum (gojiberry) on super infecting microorganisms founded in oral environment, as well as to characterize the chemical profile of the extract. To determination of Minimum Inhibitory Concentration (MIC), Minimum Inhibitory Concentration of Adherence (MICA), and bactericidal and fungicidal kinetics of the extract, Enterococcus faecalis(ATCC), Pseudomonas aeruginosa (ATCC), and Candida albicans (AB) were used. Each test was performed in duplicate and the same procedure was made to the positive control, chlorhexidine digluconate at 0,12%, and nystatin. To the statistical analysis at 5% significance level, t-Student or Mann-Whitney test was applied to the results lecture. The CCD analysis suggested the presence of phenolic acids, flavonoids, coumarins, and carbohydrates. Lycium barbarum presented bacteriostatic and fungistatic activity medium superior to the positive control of chlorexidine, and statistical significant dilution up to 1:4(125 mg/mL) and 1:16 (31,25 mg/mL) on Enterococcus faecalis ATCC and P. aeruginosa ATCC, respectively, and to 1:16 (31,25 mg/mL) to C. albicans (AB). The extract did not presented bactericidal and fungicidal action, however presented nonstick effect of dilution up to 1:32 (15,65 mg/mL) on P. aeruginosa ATCC. It may be concluded that Lycium barbarum presented significant antimicrobial action, justified by the pharmacological components, stimulating new researches about bioactive natural substances, to the treatment of persistent or refractory oral infections. |
| author2 |
Lins, Ruthineia Diógenes Alves Uchoa |
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Lins, Ruthineia Diógenes Alves Uchoa Carvalho, Isabelle Helena Gurgel de |
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bachelorThesis |
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Carvalho, Isabelle Helena Gurgel de |
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Carvalho, Isabelle Helena Gurgel de |
| title |
Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
| title_short |
Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
| title_full |
Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
| title_fullStr |
Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
| title_full_unstemmed |
Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
| title_sort |
ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. |
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Universidade Federal do Rio Grande do Norte |
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2017 |
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https://repositorio.ufrn.br/handle/123456789/39225 |
| work_keys_str_mv |
AT carvalhoisabellehelenagurgelde acaoantimicrobianadoextratodelyciumbarbarumfrenteamicrorganismossuperinfectantesdoambientebucal |
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1773961903140241408 |
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ri-123456789-392252023-05-03T14:10:05Z Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. Carvalho, Isabelle Helena Gurgel de Lins, Ruthineia Diógenes Alves Uchoa Lins, Ruthineia Diógenes Alves Uchoa Gurgel, Bruno Cesar De Vasconcelos Dametto, Fabio Roberto Fitoterapia Microbiologia e Cromatografia em camada delgada. Using of plants, extracts, and isolated metabolites is becoming very popular nowadays. Natural medicine does not acts only preventively, as also treats many diseases caused by resistant microorganisms. This study aimed to evaluate the antimicrobial action of the vegetable extract of Lycium barbarum (gojiberry) on super infecting microorganisms founded in oral environment, as well as to characterize the chemical profile of the extract. To determination of Minimum Inhibitory Concentration (MIC), Minimum Inhibitory Concentration of Adherence (MICA), and bactericidal and fungicidal kinetics of the extract, Enterococcus faecalis(ATCC), Pseudomonas aeruginosa (ATCC), and Candida albicans (AB) were used. Each test was performed in duplicate and the same procedure was made to the positive control, chlorhexidine digluconate at 0,12%, and nystatin. To the statistical analysis at 5% significance level, t-Student or Mann-Whitney test was applied to the results lecture. The CCD analysis suggested the presence of phenolic acids, flavonoids, coumarins, and carbohydrates. Lycium barbarum presented bacteriostatic and fungistatic activity medium superior to the positive control of chlorexidine, and statistical significant dilution up to 1:4(125 mg/mL) and 1:16 (31,25 mg/mL) on Enterococcus faecalis ATCC and P. aeruginosa ATCC, respectively, and to 1:16 (31,25 mg/mL) to C. albicans (AB). The extract did not presented bactericidal and fungicidal action, however presented nonstick effect of dilution up to 1:32 (15,65 mg/mL) on P. aeruginosa ATCC. It may be concluded that Lycium barbarum presented significant antimicrobial action, justified by the pharmacological components, stimulating new researches about bioactive natural substances, to the treatment of persistent or refractory oral infections. O uso de plantas, extratos e metabólitos isolados está se tornando bastante popular. Os remédios naturais não só agem profilaticamente, como também tratam muitas doenças causadas por micorganismos resistentes. Esta pesquisa teve como objetivo avaliar a ação antimicrobiana do extrato vegetal de Lycium barbarum (gojiberry) sobre microrganismos superinfectantes do meio ambiente bucal, bem como caracterizar o perfil químico do extrato.Para determinação da Concentração Inibitória Mínima (CIM), Concentração Inibitória Mínima de Aderência (CIMA) e cinética bactericida e fungicida do extrato, foram utilizados Enterococcus faecalis(ATCC), Pseudomonas aeruginosa (ATCC) e Candida albicans (AB). Cada ensaio foi realizado em duplicata e o mesmo procedimento foi realizado para o controle positivo, digluconato de clorexidina a 0,12% e nistatina. Para a análise estatística ao nível de 5% de significância foi aplicado o teste t-Student ou de Mann-Whitney para a avaliação dos resultados. A análise por cromatografia em camada delgada (CCD) sugeriu a presença de ácidos fenólicos, flavonoides, cumarinas e carboidratos. Lycium barbarum apresentou desempenho bacteriostático e fungistático médio superior ao controle positivo de clorexidina e estatisticamente significativo até a diluição 1:4 (125 mg/mL) e 1:16 (31,25 mg/mL) sobre Enterococcus faecalis ATCC e P. aeruginosa ATCC, respectivamente, e de 1:16 (31,25 mg/mL) para C. albicans (AB). O extrato não apresentou ação bactericida e fungicida, entretanto apresentou efeito antiaderente até a diluição 1:32 (15,65 mg/mL) sobre P. aeruginosa ATCC. Conclui-se que Lycium barbarum apresentou significativa ação antimicrobiana, justificada pelos constituintes farmacológicos, estimulando a pesquisa de substâncias naturais bioativas, para tratamento de infecções bucais persistentes ou refratárias. 2017-11-01T18:37:10Z 2021-09-27T14:08:39Z 2017-11-01T18:37:10Z 2021-09-27T14:08:39Z 2017-06-19 bachelorThesis 2013037870 CARVALHO, Isabelle Helena Gurgel de. Ação antimicrobiana do extrato de lycium barbarum frente a microrganismos superinfectantes do ambiente bucal. 2017. Monografia (Graduação) Departamento de Odontologia, Universidade Federal do Rio Grande do Norte, Natal, 2017. https://repositorio.ufrn.br/handle/123456789/39225 pt_BR openAccess application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN Odontologia |