Avaliação das características imunofenotípicas da Micose Fungóide e Síndrome de Sézary
Cutaneous T-cell lymphomas (LCCTs) constitute a group of extranodal lymphoproliferative diseases, currently classified and subdivided according to clinical behavior according to the WHO/EORTC. Among the CTCLs, the most common are mycosis fungoides (MF) with an indolent course and sezary syndrome (SS...
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| Formato: | article |
| Idioma: | pt_BR |
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Sociedade Brasileira de Análises Clínicas ( SBAC)
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/38941 |
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| Resumo: | Cutaneous T-cell lymphomas (LCCTs) constitute a group of extranodal lymphoproliferative diseases, currently classified and subdivided according to clinical behavior according to the WHO/EORTC. Among the CTCLs, the most common are mycosis fungoides (MF) with an indolent course and sezary syndrome (SS), which corresponds to the leukemic form of MF, generally associated with an aggressive course. The present study aimed to demonstrate the importance of immunophenotyping by flow cytometry through the positivity and intensity of antigenic expressions in confirming the diagnosis of mycosis fungoides and Sézary syndrome. Peripheral blood (SP) samples from 23 patients previously diagnosed with MF were analyzed in order to investigate SS. These patients were referred to the Laboratory of the Department of Hematology, Blood Center Dalton Barbosa Cunha (HEMONORTE), Natal, Brazil. Patients were analyzed for clinical, hematological and immunophenotypic aspects, using 4.0 mL of peripheral blood (SP). In immunophenotyping by flow cytometry (CF), specific panels of AcMo conjugated directly to three different fluorochromes were used: fluorescein isothiocyanate (FITC) Phicoerythrin (PE), chlorophyll pyridine protein, (PerCP), which detect green, orange and red fluorescence, respectively, allowing the labeling of up to three labels per tube, consisting of pan-B, pan-T and unspecified antigens. In our study, the CF analysis made it possible to identify the predominance of the population of mature T cells (CD3+/CD2+/CD5+, TCRAB+) with expansion of
CD4+ cells in 100% of cases. Through Sézary cell (CS) analysis, patients numbers 1 and 3 were identified ( table 3 ) with absolute CS counts of less than 1,000/mm³, however, the other patients had absolute CS values > 1,000/mm³ of SP. In the results obtained, we observed that CF immunophenotyping proved to be an efficient methodology in the detection of CS cells, which can be used in the early diagnosis and monitoring of MS/SS. |
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