Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)

Due to the increase in bacterial resistance in recent years, research and development of alternative antibacterial therapies has become necessary and, in this sense, the use of magnetic fields has been explored as it is a non-pharmacological therapy and, therefore, probably exempt of the molecula...

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Autor principal: Santos, Sunamita da Silva Barbosa
Outros Autores: Silva, Marcelo de Sousa da
Formato: Dissertação
Idioma:pt_BR
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
Resistência bacteriana
Magnetita
Campo magnético
Endereço do item:https://repositorio.ufrn.br/handle/123456789/38016
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id ri-123456789-38016
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language pt_BR
topic Resistência bacteriana
Magnetita
Campo magnético
spellingShingle Resistência bacteriana
Magnetita
Campo magnético
Santos, Sunamita da Silva Barbosa
Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)
description Due to the increase in bacterial resistance in recent years, research and development of alternative antibacterial therapies has become necessary and, in this sense, the use of magnetic fields has been explored as it is a non-pharmacological therapy and, therefore, probably exempt of the molecular effects of the resistance phenomenon. At the same time, nanoparticles with magnetic properties, especially magnetite (Fe3O4), are still little explored in terms of their antibacterial potential. In this context, the present work evaluated the intrinsic antibacterial properties of magnetite nanoparticles, synthesized by high-energy milling, and their association with a variable external magnetic field of 70 mT at 10 Hz on Escherichia coli DH5α pVAX-1. After synthesis, the nanoparticles were characterized as to their composition and primary size, by X-ray diffraction (XRD); as to its hydrodynamic size, by dynamic light scattering (DLS) and as to its surface charge, by zeta potential. Subsequently, in vitro assays were performed, in which alterations in the bacterial inhibition curve were investigated; in bacterial morphology, using microscopy (SEM), and in bacterial surface load, using zeta potential. The association between the antibiotic kanamycin and magnetite was also evaluated regarding the potentiated effect between the substances. Exposure to isolated magnetite, or combined to a magnetic field, generated significant bacteriostatic effects, in addition to morphological changes in the bacterial surface and an increase in the zeta potential of the suspension. The use of magnetite nanoparticles also potentiated the inhibitory effect of the antibiotic kanamycin, pointing to a second option for use as an adjunct to antibiotic therapy. The results presented reveal important antimicrobial potential and additional in vivo tests are needed to prove the importance of the effects observed in a biological system.
author2 Silva, Marcelo de Sousa da
author_facet Silva, Marcelo de Sousa da
Santos, Sunamita da Silva Barbosa
format masterThesis
author Santos, Sunamita da Silva Barbosa
author_sort Santos, Sunamita da Silva Barbosa
title Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)
title_short Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)
title_full Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)
title_fullStr Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)
title_full_unstemmed Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina)
title_sort atividade antibacteriana de magnetita (fe3o4) isolada e associada a campos eletromagnéticos sobre escherichia coli dh5a pvax-1 (resistente à canamicina)
publisher Universidade Federal do Rio Grande do Norte
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/38016
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AT santossunamitadasilvabarbosa antibacterialactivityofmagnetitefe3o4isolatedandassociatedwithelectromagneticfieldsonescherichiacolidh5apvax1resistanttokanamycin
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spelling ri-123456789-380162022-05-02T15:08:39Z Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina) Antibacterial activity of magnetite (Fe3O4) isolated and associated with electromagnetic fields on Escherichia coli DH5α pVAX-1 (resistant to kanamycin) Santos, Sunamita da Silva Barbosa Silva, Marcelo de Sousa da http://lattes.cnpq.br/6988181736543670 http://lattes.cnpq.br/1295430560645312 Melo, Maria Celeste Nunes de http://lattes.cnpq.br/0580551464788795 Barbosa, Júlio César Pereira http://lattes.cnpq.br/0437190557200470 Resistência bacteriana Magnetita Campo magnético Due to the increase in bacterial resistance in recent years, research and development of alternative antibacterial therapies has become necessary and, in this sense, the use of magnetic fields has been explored as it is a non-pharmacological therapy and, therefore, probably exempt of the molecular effects of the resistance phenomenon. At the same time, nanoparticles with magnetic properties, especially magnetite (Fe3O4), are still little explored in terms of their antibacterial potential. In this context, the present work evaluated the intrinsic antibacterial properties of magnetite nanoparticles, synthesized by high-energy milling, and their association with a variable external magnetic field of 70 mT at 10 Hz on Escherichia coli DH5α pVAX-1. After synthesis, the nanoparticles were characterized as to their composition and primary size, by X-ray diffraction (XRD); as to its hydrodynamic size, by dynamic light scattering (DLS) and as to its surface charge, by zeta potential. Subsequently, in vitro assays were performed, in which alterations in the bacterial inhibition curve were investigated; in bacterial morphology, using microscopy (SEM), and in bacterial surface load, using zeta potential. The association between the antibiotic kanamycin and magnetite was also evaluated regarding the potentiated effect between the substances. Exposure to isolated magnetite, or combined to a magnetic field, generated significant bacteriostatic effects, in addition to morphological changes in the bacterial surface and an increase in the zeta potential of the suspension. The use of magnetite nanoparticles also potentiated the inhibitory effect of the antibiotic kanamycin, pointing to a second option for use as an adjunct to antibiotic therapy. The results presented reveal important antimicrobial potential and additional in vivo tests are needed to prove the importance of the effects observed in a biological system. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Devido ao aumento da resistência bacteriana nos últimos anos, tem tornado necessário a pesquisa e desenvolvimento de terapias antibacterianas alternativas e, nesse sentido, o uso de campo magnéticos tem sido explorados por tratar-se de uma terapia não farmacológica e, por isso, provavelmente isenta dos efeitos moleculares do fenômeno de resistência. Paralelamente, nanopartículas com propriedades magnéticas, em especial a magnetita (Fe3O4), são ainda pouco exploradas quanto ao potencial antibacteriano. Nesse contexto, o presente trabalho avaliou as propriedades antibacterianas intrínsecas de nanopartículas de magnetita, sintetizadas por moagem de alta energia, e da sua associação com um campo magnético externo variável de 70 mT a 10 Hz sobre Escherichia coli DH5α pVAX-1. Após a síntese, as nanopartículas foram caracterizadas quanto sua composição e tamanho primário, por difração de raio-X (DRX); quanto ao seu tamanho hidrodinâmico, por espalhamento dinâmico de luz (DLS) e quanto à sua carga superficial, por potencial zeta. Posteriormente, os ensaios in vitro foram realizados, nos quais foram investigadas alterações na curva de inibição bacteriana; na morfologia bacteriana, por meio de microscopia (MEV), e na carga de superfície bacteriana, utilizando potencial zeta. A associação entre o antibiótico canamicina e magnetita também foi avaliado quanto ao efeito potencializado entre as substâncias. A exposição à magnetita isolada, ou combinada ao campo magnético gerou efeitos bacteriostáticos significativos, além de alterações morfológicas na superfície bacteriana e aumento no potencial zeta da suspensão. O uso de nanopartículas de magnetita também potencializou o efeito inibitório do antibiótico canamicina, apontando para o uma segunda opção de uso, como coadjuvante na terapia antibiótica. Os resultados apresentados revelam potencial antimicrobiano importante e ensaios in vivo adicionais são necessários para comprovar a importância dos efeitos observados num sistema biológico. 2021-09-24T15:52:05Z 2021-09-24T15:52:05Z 2021-06-30 masterThesis SANTOS, Sunamita da Silva Barbosa. Atividade antibacteriana de magnetita (Fe3O4) isolada e associada a campos eletromagnéticos sobre Escherichia coli DH5a pVAX-1 (resistente à canamicina). 2021. 85f. Dissertação (Mestrado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal. https://repositorio.ufrn.br/handle/123456789/38016 pt_BR Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN PROGRAMA DE PÓS-GRADUAÇÃO EM BIOQUÍMICA

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