Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia

CNPq, UFRN

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Autor principal: Lima, Adrielle Kétcia Alves de
Outros Autores: Ururahy, Marcela Abbott Galvão
Formato: bachelorThesis
Idioma:English
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
podocin
wilms tumor 1
biomarker
podocyte
pregnancy
Bioquímica Clínica
Endereço do item:https://repositorio.ufrn.br/handle/123456789/35825
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spelling ri-123456789-358252021-09-20T17:52:34Z Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia Lima, Adrielle Kétcia Alves de Ururahy, Marcela Abbott Galvão Ururahy, Marcela Abbott Galvão Souza, Karla Simone Costa de Domingos, Igor de Farias podocin wilms tumor 1 biomarker podocyte pregnancy Bioquímica Clínica CNPq, UFRN Background: Preeclampsia is a systemic syndrome that affects pregnancy, characterized by increased blood pressure and, frequently, proteinuria. It is considered one of the main causes of maternal mortality and due to its possible serious complications, a predictive diagnostic tool is required. There has been increasing evidence that podocytes are related to kidney damage associated to preeclampsia and that the presence of podocyturia was found to be highly sensitive and specific to this disease. Objective: To characterize the expression profile of the podocyte proteins nephrin, podocin and Wilms’ tumor 1 (WT-1) present in the urinary extracellular vesicles of pregnant women diagnosed with severe preeclampsia in order to investigate their potential use as biomarkers in the early diagnosis of the disease. Study Design: This is a cross-sectional study, including 20 pregnant women divided into two groups. The first group with 10 pregnant women diagnosed with severe preeclampsia (case group - PE) and the second, with 10 normotensive pregnant women (control group - CG). Fasting blood samples were used to evaluate the patients' overall metabolic status. First morning urine samples were collected to determinate the albumin/creatinine ratio and to isolate urinary extracellular vesicles by ultracentrifugation. Nephrin, podocin and WT-1 expressions were evaluated by Western-blot. Results: Increased serum creatinine (p = 0.015), uric acid (p <0.001) and urinary albumin/creatinine ratio (p <0.001), as well as decreased serum albumin (p <0.001), total protein (p = 0.037) and calcium (p = 0.003) were found in case group when compared control group. Regarding the podocyte proteins in the urinary extracellular vesicles, significant increased nephrin/creatinine ratio (p = 0.008) was observed in preeclampsia when compared to those from normotensive pregnant women. In addition, the area under de Receiver Operating Characteristic Curve (AUROC) analysis showed that nephrin/creatinine ratio is a good predictor of albuminuria (AUROC = 0.833; p = 0.018). Conclusion: Increase of the nephrin in the urinary extracellular vesicles in pregnant women with preeclampsia proves to be a potential predictor for the early diagnosis of the disease. 2020-07-29T10:58:34Z 2021-09-20T17:52:34Z 2020-07-29T10:58:34Z 2021-09-20T17:52:34Z 2020-07-20 bachelorThesis 20170014211 LIMA, Adrielle Kétcia Alves de. Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia. 2020. 49 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal, 2020. https://repositorio.ufrn.br/handle/123456789/35825 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/octet-stream Universidade Federal do Rio Grande do Norte Brasil UFRN Farmácia
institution Repositório Institucional
collection RI - UFRN
language English
topic podocin
wilms tumor 1
biomarker
podocyte
pregnancy
Bioquímica Clínica
spellingShingle podocin
wilms tumor 1
biomarker
podocyte
pregnancy
Bioquímica Clínica
Lima, Adrielle Kétcia Alves de
Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
description CNPq, UFRN
author2 Ururahy, Marcela Abbott Galvão
author_facet Ururahy, Marcela Abbott Galvão
Lima, Adrielle Kétcia Alves de
format bachelorThesis
author Lima, Adrielle Kétcia Alves de
author_sort Lima, Adrielle Kétcia Alves de
title Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
title_short Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
title_full Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
title_fullStr Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
title_full_unstemmed Increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
title_sort increased nephrin in urinary extracellular vesicles of pregnant women with severe preeclampsia
publisher Universidade Federal do Rio Grande do Norte
publishDate 2020
url https://repositorio.ufrn.br/handle/123456789/35825
work_keys_str_mv AT limaadrielleketciaalvesde increasednephrininurinaryextracellularvesiclesofpregnantwomenwithseverepreeclampsia
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