Caracterização da imunogenicidade de antígenos recombinantes de Trypanosoma cruzi em modelo murino
Chagas disease (CD) is a neglected tropical disease caused by the protozoan Trypanosoma cruzi. Numerous parasite proteins are being purified and used as antigenic candidates for the development of vaccines in immunization protocols. In this context, an immunization protocol was developed with a m...
Na minha lista:
| Autor principal: | |
|---|---|
| Outros Autores: | |
| Formato: | bachelorThesis |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
|
| Assuntos: | |
| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/35810 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
| Resumo: | Chagas disease (CD) is a neglected tropical disease caused by the protozoan Trypanosoma
cruzi. Numerous parasite proteins are being purified and used as antigenic candidates for the
development of vaccines in immunization protocols. In this context, an immunization
protocol was developed with a mixture of two recombinant antigens called X (FPPSTc) and Y
(SPPSTc) in the presence and absence of the aluminum hydroxide adjuvant, in order to
characterize its immunostimulatory capacity. BALB / c mice were immunized and blood
samples were obtained at 15, 30 and 45 days after immunization. The profile of specific
antibodies total IgG, IgG1, IgG2a, IgG2b and IgG3 were determined by immunoassay. The
data demonstrate the highest production of total anti-X IgG, especially when associated with
aluminum hydroxide, whereas the production of anti-Y antibodies was not significant in
relation to the negative control. In addition, the mixture of the two antigens showed a better
profile for the IgG2b subclass. Additionally, it was observed that sera from chagasic patients
do not recognize the X and Y antigens and that the sera from the immunized animals do not
recognize the total T. cruzi extract. In conclusion, this study contributes to the understanding
of the immunogenicity of these two possible antigenic candidates for the rational development
of vaccines in the context of Chagas disease. |
|---|