Método RT-LAMP na detecção molecular de SARS-CoV, MERS-CoV e SARS-CoV-2.
RT-LAMP has shown itself to be a promising tool due to its simple technology, high sensitivity / specificity and reduced diagnosis time. Based on the nucleic acid amplification test, this method proved to be eligible for the molecular detection of respiratory viruses, which implies its possible a...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/35804 |
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| Resumo: | RT-LAMP has shown itself to be a promising tool due to its simple technology,
high sensitivity / specificity and reduced diagnosis time. Based on the nucleic acid
amplification test, this method proved to be eligible for the molecular detection of
respiratory viruses, which implies its possible applicability in the detection of
Coronavirus. This study gathered data on the molecular detection by RT-LAMP of
SARS-CoV, MERS-CoV and SARS-CoV-2, analyzing and comparing their
methodological applicability and the respective evolution in the diagnosis of these
viruses. SARS-CoV and SARS-CoV-2 studies used upper respiratory tract samples, and
those of MERS-CoV, utilized cells cultured and infected in laboratory. As main target
regions all use the ORF1 region, however, MERS-CoV and SARS-CoV-2 also use the E
region, and the N and RdRp regions, respectively. |
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