Citotoxicidade e genotoxicidade do reteno: um hidrocarboneto policíclico aromático marcador da queima de biomassa
Retene (RET) is a polycyclic aromatic hydrocarbon (PAH) released from biomass burning and used as a biomarker of coniferous wood combustion in the air environment. Although not considered as one of the priority HPAs and not included in risk assessments by the US Environmental Protection Agency (US-E...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/35775 |
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| Resumo: | Retene (RET) is a polycyclic aromatic hydrocarbon (PAH) released from biomass burning and used as a biomarker of coniferous wood combustion in the air environment. Although not considered as one of the priority HPAs and not included in risk assessments by the US Environmental Protection Agency (US-EPA), the few studies that exist with RET indicate that is capable of generating oxidative stress, causing cytotoxicity. In order to contribute to the elucidation of its function, the present study aimed to evaluate whether RET, in concentrations similar to those found in the environment, causes cellular and genetic damage in chinese hamster ovary (CHO) cells. For this, two experiments were performed: the cell viability test by the MTT reduction rate in formazan crystals and the Cytokinesis Block Micronucleus Cytome assay (CBMN cyt). Results revealed a decrease in viable cells at higher RET concentrations during 24 hours of treatment, yielding a dose-dependent response. Exposure during that time led to a significant increase in the frequency of micronuclei (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) as the concentration of this pollutant increased. Thus, the importance of including RET in routine environmental analysis in the future is emphasized. |
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