Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.

Sporotrichosis, caused by fungi belonging to the Sporothrix schenckii complex, is widely distributed around the globe, especially in tropical and subtropical regions. Traumatic inoculation is the usual route of acquisition. This infectious disease used to be restricted to farmers and armadillo’s hun...

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Autor principal: Costa, Alexandre Soares de Sena
Outros Autores: Chaves, Guilherme Maranhão
Formato: bachelorThesis
Idioma:English
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
Sporothrix spp.
sporotrichosis
cats
zoonosis
subcutaneous mycosis
Endereço do item:https://repositorio.ufrn.br/handle/123456789/35740
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id ri-123456789-35740
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language English
topic Sporothrix spp.
sporotrichosis
cats
zoonosis
subcutaneous mycosis
spellingShingle Sporothrix spp.
sporotrichosis
cats
zoonosis
subcutaneous mycosis
Costa, Alexandre Soares de Sena
Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
description Sporotrichosis, caused by fungi belonging to the Sporothrix schenckii complex, is widely distributed around the globe, especially in tropical and subtropical regions. Traumatic inoculation is the usual route of acquisition. This infectious disease used to be restricted to farmers and armadillo’s hunters due to vegetable manipulation injuries. However, studies in Brazil have reported frequent outbreaks associated with feline transmission, characterizing sporotrichosis as a zoonotic infection. This study aimed to isolate, purify and evaluate in vitro virulence factors (colony growth and melanin production at different glucose concentrations), as well as, antifungal susceptibility testing to itraconazole (ITZ) and amphotericin B (AmB) of 40 clinical isolates obtained from patients living in Rio Grande do Norte state, Brazil. In our study, 75% of the patients were female and two clinical forms of infection were predominant, cutaneous-fixed and lymphocutaneous. We also report a case of primary pulmonary sporotrichosis leading to patient’s death. The molecular screening revealed that 12 strains were identified as S. brasiliensis. All the isolates were susceptible to both antifungal drugs tested, with MIC values ranging from 0.0312 to 0.5 μg/mL for ITZ and 0.125 and 1 μg/mL for AmB. There was no remarkable conclusion for colony growth, but a low number of strains produced melanin. Our study shows a prevalence of cases of sporotrichosis in female patients and S. brasiliensis as an etiological agent of infection in the region. In addition, the isolates demonstrated a satisfactory antifungal susceptibility profiling, with low MIC values to the antifungal drugs tested.
author2 Chaves, Guilherme Maranhão
author_facet Chaves, Guilherme Maranhão
Costa, Alexandre Soares de Sena
format bachelorThesis
author Costa, Alexandre Soares de Sena
author_sort Costa, Alexandre Soares de Sena
title Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
title_short Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
title_full Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
title_fullStr Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
title_full_unstemmed Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex.
title_sort isolation, identification and phenotypic characterization of clinical isolates of the sporothrix schenckii complex.
publisher Universidade Federal do Rio Grande do Norte
publishDate 2019
url https://repositorio.ufrn.br/handle/123456789/35740
work_keys_str_mv AT costaalexandresoaresdesena isolationidentificationandphenotypiccharacterizationofclinicalisolatesofthesporothrixschenckiicomplex
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spelling ri-123456789-357402021-09-20T17:50:17Z Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex. Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex. Costa, Alexandre Soares de Sena Chaves, Guilherme Maranhão Ururahy, Marcela Abbott Galvão Souza, Luanda Bárbara Ferreira Canário de Sporothrix spp. sporotrichosis cats zoonosis subcutaneous mycosis Sporotrichosis, caused by fungi belonging to the Sporothrix schenckii complex, is widely distributed around the globe, especially in tropical and subtropical regions. Traumatic inoculation is the usual route of acquisition. This infectious disease used to be restricted to farmers and armadillo’s hunters due to vegetable manipulation injuries. However, studies in Brazil have reported frequent outbreaks associated with feline transmission, characterizing sporotrichosis as a zoonotic infection. This study aimed to isolate, purify and evaluate in vitro virulence factors (colony growth and melanin production at different glucose concentrations), as well as, antifungal susceptibility testing to itraconazole (ITZ) and amphotericin B (AmB) of 40 clinical isolates obtained from patients living in Rio Grande do Norte state, Brazil. In our study, 75% of the patients were female and two clinical forms of infection were predominant, cutaneous-fixed and lymphocutaneous. We also report a case of primary pulmonary sporotrichosis leading to patient’s death. The molecular screening revealed that 12 strains were identified as S. brasiliensis. All the isolates were susceptible to both antifungal drugs tested, with MIC values ranging from 0.0312 to 0.5 μg/mL for ITZ and 0.125 and 1 μg/mL for AmB. There was no remarkable conclusion for colony growth, but a low number of strains produced melanin. Our study shows a prevalence of cases of sporotrichosis in female patients and S. brasiliensis as an etiological agent of infection in the region. In addition, the isolates demonstrated a satisfactory antifungal susceptibility profiling, with low MIC values to the antifungal drugs tested. Sporotrichosis, caused by fungi belonging to the Sporothrix schenckii complex, is widely distributed around the globe, especially in tropical and subtropical regions. Traumatic inoculation is the usual route of acquisition. This infectious disease used to be restricted to farmers and armadillo’s hunters due to vegetable manipulation injuries. However, studies in Brazil have reported frequent outbreaks associated with feline transmission, characterizing sporotrichosis as a zoonotic infection. This study aimed to isolate, purify and evaluate in vitro virulence factors (colony growth and melanin production at different glucose concentrations), as well as, antifungal susceptibility testing to itraconazole (ITZ) and amphotericin B (AmB) of 40 clinical isolates obtained from patients living in Rio Grande do Norte state, Brazil. In our study, 75% of the patients were female and two clinical forms of infection were predominant, cutaneous-fixed and lymphocutaneous. We also report a case of primary pulmonary sporotrichosis leading to patient’s death. The molecular screening revealed that 12 strains were identified as S. brasiliensis. All the isolates were susceptible to both antifungal drugs tested, with MIC values ranging from 0.0312 to 0.5 μg/mL for ITZ and 0.125 and 1 μg/mL for AmB. There was no remarkable conclusion for colony growth, but a low number of strains produced melanin. Our study shows a prevalence of cases of sporotrichosis in female patients and S. brasiliensis as an etiological agent of infection in the region. In addition, the isolates demonstrated a satisfactory antifungal susceptibility profiling, with low MIC values to the antifungal drugs tested. 2019-11-19T16:49:51Z 2021-09-20T17:50:17Z 2019-11-19T16:49:51Z 2021-09-20T17:50:17Z 2019-11-05 bachelorThesis 20150120772 COSTA, Alexandre Soares de Sena. Isolation, identification and phenotypic characterization of clinical isolates of the Sporothrix schenckii Complex. 2019. 33 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2019. https://repositorio.ufrn.br/handle/123456789/35740 en Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN Graduação em Farmácia

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