Atividade antibacteriana de carboidratos extraídos de Gracilaria birdiae cultivadas na praia de Pitangui-Extremoz-RN sobre biofilmes bacterianos
Algae cultivation provides conditions that combine biodiversity protection through better environmental conditions so that traditional populations of fishermen and algae producers can develop their activities and ensure their socioeconomic and cultural sustainability. Gracilaria birdiae is a rodophi...
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| Formato: | bachelorThesis |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/33919 |
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| Resumo: | Algae cultivation provides conditions that combine biodiversity protection through better environmental conditions so that traditional populations of fishermen and algae producers can develop their activities and ensure their socioeconomic and cultural sustainability. Gracilaria birdiae is a rodophita (red algae) which produces agar polymers, have shown several relevant biological activities, such as antimicrobial activity, for example. Many bacterial infections are associated with the ability of these microorganisms to form biofilms, which is essential for colonization of the most diverse environments, as well as conferring high levels of resistance. In this sense, the discovery of new molecules that have antimicrobial effect or are able to inhibit the formation of biofilms, constitutes as a target to be reached. The objective of this work was to evaluate the antibacterial and antibiofilm activity of carbohydrates extracted from G. bidiae on infectionrelated bacteria. The algae from the cultivation were washed with running water, were later lined in wood paper and dried in an aerated and crushed greenhouse. Delipidation and proteolysis (removal of pigmentation and proteins) were performed. After this process, the filtering was performed to remove only the liquid. 950ml of liquid was obtained, added twice as methanol for the formation of the extract. Erlenmeyers with liquid were on ice for 1 day. The next step was centrifugation at 10,000 rpm at 4 ° C. To determine antibacterial activity, carbohydrates extracted from the algae were diluted to concentrations ranging from 1000 to 15.6 μg / mL and distributed in 96-well flat-bottom polystyrene plates with each adjusted bacterial suspension (1 x 10⁶ CFU / mL) in TSB medium and then incubated for 24 hours at 37 ° C. Susceptibility of microorganisms to carbohydrates was evaluated by tests and minimum inhibitory concentration (MIC), the effect on biofilms was evaluated by quantification of biomass by the crystal violet staining method (CV) and by the number of viable cells of the biofilm by colony forming unit count (CFU). The results showed a significant reduction of Staphylococcus aureus biofilm biomass at concentrations of 62.5 μg / mL to 1000 μg / mL, and at concentrations of 62.5 μg / mL μg / mL at 500 on Escherichia coli. However, no significant activity on Staphylococcus epidermidis was observed. Regarding the number of viable cells in the biofilms, carbohydrates did not present significant difference in relation to the control. The results obtained in this work suggest that carbohydrates extracted from G. birdiae have a promising action on Gram-positive and Gram-negative bacteria biofilms. |
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