Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10

Cancer is a multifactorial disease that comes up from disorderly growth of atypical cells resulting in tissue invasions that lead to tumor formation. Among the of the most diverse types of existing cancers, melanoma-type skin cancer is one of the most dangerous, especially in countries with a tro...

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Autor principal: Ferreira, Macelia Pinheiro
Outros Autores: Almeida, Maria das Graças
Formato: bachelorThesis
Idioma:pt_BR
Publicado em: Universidade Federal do Rio Grande do Norte
Assuntos:
Câncer. Chá verde (Camellia sinensis). EGCG. Melanoma.
Cancer. Green tea (Camellia sinensis). EGCG. Melanona.
Endereço do item:https://repositorio.ufrn.br/handle/123456789/33356
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id ri-123456789-33356
record_format dspace
institution Repositório Institucional
collection RI - UFRN
language pt_BR
topic Câncer. Chá verde (Camellia sinensis). EGCG. Melanoma.
Cancer. Green tea (Camellia sinensis). EGCG. Melanona.
spellingShingle Câncer. Chá verde (Camellia sinensis). EGCG. Melanoma.
Cancer. Green tea (Camellia sinensis). EGCG. Melanona.
Ferreira, Macelia Pinheiro
Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10
description Cancer is a multifactorial disease that comes up from disorderly growth of atypical cells resulting in tissue invasions that lead to tumor formation. Among the of the most diverse types of existing cancers, melanoma-type skin cancer is one of the most dangerous, especially in countries with a tropical climate, duo to the high incidence of sunlight. Based on this, several treatments are studied around the world, with the aim of improving the effectiveness and quality of life of patients who suffer from the side effects of conventional cancer treatments. Seeking to reduce the adverse effects resulting from usual antineoplastic molecules, several studies have investigated the effect of plants on tumor progession and on the hypercoagulable state that is associated with cancer. Among the most studied medicinal plants currently is Camellia sinensis, from which green tea is extracted and EGCG (Major compound of green tea). Based on the above, the aim of this study was to evaluate the effect of the green tea and EGCG on hemostasis and melanoma tumor development, which may be contributing as an adjuvant in traditional cancer treatment. The present study evaluated the antitumor, antiangiogenic and anticoagulant properties of green teat and EGCG. During the various experiments carried out, the tested concentrations of green tea and EGCG were 25μg/mL, 50μg/mL e 100μg/mL. It was observed in the platelet aggregation test a potent effect of green tea decreasing by up to 50% and EGCG decreasing by about 85% when compared to the negative control. The evaluation of the effects of green tea and EGCG on cell death through the apoptosis test by incubation with DAPI revealed a reduction of around 25% and 50% of green tea and EGCG, respectively. During the angiogenesis experiment performed with endothelial cells, there was a reduction in the formation of new blood vessels of about 25% in the presence of green tea and about 99% in the presence of EGCG at concentrations of 100μg/mL; In the study of the possible effect of green tea and MCGT on the reduction of VEGF and IL-8 secretion, VEGF and IL-8 production were inhibited in the presence of green tea by 55% and 20% respectively; and in the presence of EGCG there was a 75% reduction in VEGF inhibition and about 80% in the inhibition of IL-8 secretion; in both cases at a concentration of 100μg/mL. Therefore, the effects of green tea and EGCG on primary hemostasis and tumor development are evidenced through the results, and further studies are needed to clarify the action of these substances.
author2 Almeida, Maria das Graças
author_facet Almeida, Maria das Graças
Ferreira, Macelia Pinheiro
format bachelorThesis
author Ferreira, Macelia Pinheiro
author_sort Ferreira, Macelia Pinheiro
title Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10
title_short Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10
title_full Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10
title_fullStr Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10
title_full_unstemmed Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10
title_sort efeitos do chá verde (camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células b16-f10
publisher Universidade Federal do Rio Grande do Norte
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/33356
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spelling ri-123456789-333562021-09-10T19:07:38Z Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10 Ferreira, Macelia Pinheiro Almeida, Maria das Graças 0000-0001-7756-0442 http://lattes.cnpq.br/6876018099354505 0000-0002-5587-0922 http://lattes.cnpq.br/0321740024191482 Silva, Saulo Victor e 0000-0003-0793-2647 http://lattes.cnpq.br/8278651522984535 Luz, Jefferson Romáryo Duarte da 0000-0002-8249-7617 http://lattes.cnpq.br/7631213043778420 Ferrari, Márcio 0000-0002-2425-7586 http://lattes.cnpq.br/5782539548696465 Câncer. Chá verde (Camellia sinensis). EGCG. Melanoma. Cancer. Green tea (Camellia sinensis). EGCG. Melanona. Cancer is a multifactorial disease that comes up from disorderly growth of atypical cells resulting in tissue invasions that lead to tumor formation. Among the of the most diverse types of existing cancers, melanoma-type skin cancer is one of the most dangerous, especially in countries with a tropical climate, duo to the high incidence of sunlight. Based on this, several treatments are studied around the world, with the aim of improving the effectiveness and quality of life of patients who suffer from the side effects of conventional cancer treatments. Seeking to reduce the adverse effects resulting from usual antineoplastic molecules, several studies have investigated the effect of plants on tumor progession and on the hypercoagulable state that is associated with cancer. Among the most studied medicinal plants currently is Camellia sinensis, from which green tea is extracted and EGCG (Major compound of green tea). Based on the above, the aim of this study was to evaluate the effect of the green tea and EGCG on hemostasis and melanoma tumor development, which may be contributing as an adjuvant in traditional cancer treatment. The present study evaluated the antitumor, antiangiogenic and anticoagulant properties of green teat and EGCG. During the various experiments carried out, the tested concentrations of green tea and EGCG were 25μg/mL, 50μg/mL e 100μg/mL. It was observed in the platelet aggregation test a potent effect of green tea decreasing by up to 50% and EGCG decreasing by about 85% when compared to the negative control. The evaluation of the effects of green tea and EGCG on cell death through the apoptosis test by incubation with DAPI revealed a reduction of around 25% and 50% of green tea and EGCG, respectively. During the angiogenesis experiment performed with endothelial cells, there was a reduction in the formation of new blood vessels of about 25% in the presence of green tea and about 99% in the presence of EGCG at concentrations of 100μg/mL; In the study of the possible effect of green tea and MCGT on the reduction of VEGF and IL-8 secretion, VEGF and IL-8 production were inhibited in the presence of green tea by 55% and 20% respectively; and in the presence of EGCG there was a 75% reduction in VEGF inhibition and about 80% in the inhibition of IL-8 secretion; in both cases at a concentration of 100μg/mL. Therefore, the effects of green tea and EGCG on primary hemostasis and tumor development are evidenced through the results, and further studies are needed to clarify the action of these substances. O câncer é uma doença multifatorial que surge a partir do crescimento desordenado de células atípicas, resultando em invasões teciduais que levam à formação de tumores. Entre os mais diversos tipos de cânceres existentes, o câncer de pele do tipo melanoma é um dos mais perigosos. Com base nisso, diversos tratamentos são estudados em todo o mundo, com o intuito de melhorar a eficácia e a qualidade de vida dos pacientes oncológicos. Buscando a diminuição dos efeitos adversos resultantes dos tratamentos convencionais diversos estudos investigam o efeito de plantas sobre a progressão tumoral e sobre o estado de hipercoagulabilidade que está associado ao câncer. Dentre as plantas medicinais mais estudadas atualmente está a Camellia sinensis, que a partir dela é extraído o chá verde e a epigalocatequina-3galato (EGCG), composto majoritário do chá verde. Com base no exposto, o objetivo deste trabalho foi avaliar o efeito do chá verde e da EGCG sobre a hemostasia e o desenvolvimento tumoral do melanoma, podendo estar contribuindo de maneira adjuvante no tratamento oncológico tradicional. O presente estudo avaliou as propriedades antitumorais, antiangiogênicas e efeitos na hemostasia primária e secundária do chá verde e da EGCG objetivando o desenvolvimento de novas alternativas de tratamentos adjuvantes na oncologia e em alterações na hemostasia. Durante os diversos experimentos realizados, as concentrações testadas do chá verde e da EGCG foram para cada um 25μg/mL, 50μg/mL e 100μg/mL. Foi observado no teste de agregação plaquetária um potente efeito do chá verde diminuindo em até 50% e da EGCG reduzindo em cerca de 80% quando comparado ao controle negativo. A avaliação dos efeitos do chá verde e EGCG sobre a morte celular através do teste de apoptose por incubação com DAPI revelou redução em torno de 25% e 50% do chá verde e EGCG, respectivamente. Durante o experimento de angiogênese realizado com células endoteliais, observou-se redução na formação de novos vasos sanguíneos em cerca de 25% na presença do chá verde e cerca de 99% na presença da EGCG nas concentrações de 100μg/mL; No estudo do possível efeito do chá verde e EGCG sobre redução da secreção de VEGF e IL-8 a produção de VEGF e IL-8 foram inibidas na presença do chá verde em 55% e 20% respectivamente; e na presença da EGCG houve redução de 75% de inibição de VEGF e cerca de 80% na inibição de secreção de IL-8; em ambos os casos na concentração de 100μg/mL. Portanto, fica evidenciado através dos resultados os efeitos do chá verde e EGCG sobre hemostasia primária e desenvolvimento tumoral, sendo necessário mais estudos para esclarecer a ação dessas substâncias. 2021-09-10T19:07:37Z 2021-09-10T19:07:37Z 2021-08-25 bachelorThesis FERREIRA, Macelia Pinheiro. Efeitos do chá verde (Camellia sinensis) e da epigalocatequina-3-galato sobre a hemostasia e o desenvolvimento tumoral do melanoma em células B16-F10. 2021. 50 f. Trabalho de Conclusão de Curso (Graduação em Farmácia) - Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, 2021. https://repositorio.ufrn.br/handle/123456789/33356 pt_BR Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Universidade Federal do Rio Grande do Norte Brasil UFRN Farmácia Departamento de Análises Clínicas e Toxicológicas

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