Susceptibilidade do Triatoma brasiliensis (Hemiptera: Reduviidae Triatominae) à infecção por isolados do Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) do Estado do Rio Grande do Norte
The susceptibility of Triatoma brasiliensis to infection by Trypanosoma cruzi DTUs I, II and III found in the State of Rio Grande do Norte was evaluated experimentally. Nymphs were fed using direct and indirect xenodiagnosis with the isolates 3188 (TcI), RN79/RN2 (TcII) and Pl1.10.14 (TcIII) in s...
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| Formato: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/33002 |
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| Resumo: | The susceptibility of Triatoma brasiliensis to infection by Trypanosoma cruzi DTUs I, II
and III found in the State of Rio Grande do Norte was evaluated experimentally. Nymphs
were fed using direct and indirect xenodiagnosis with the isolates 3188 (TcI), RN79/RN2
(TcII) and Pl1.10.14 (TcIII) in simple or mixed infections. The intestinal contents were
evaluated by direct exam, xenoculture and kDNA polymerase chain reaction (PCR).
Then, the characterization of isolates was used the cytochrome oxidade subunit 2 gene
(COII), 24Sα ribosomal DNA gene (rDNA) and the spliced-leader intergenic region of
the mini-exon gene (SL-IR) as markers. In the direct xenodiagnosis, the direct exam
presented 30.0%(15/50) of positivity, the xenoculture 18.0%(9/50) and the kDNA PCR
88.0% (44/50). Meanwhile, the indirect xenodiagnosis showed 5.4%(3/56) of positivity to
the direct exam, 25.0%(14/56) to xenodiagnosis and 41.1%(23/56) to kDNA PCR.
Comparing the techniques, the direct xenodiagnosis showed better results than the
indirect procedure. The PCR identified the T. cruzi in both types of infection, with higher
positivity than direct exam and xenoculture. With respect the molecular characterization,
the re-isolates showed the same profile before and after the simple infections. In the
mixed infections were verified two or only one isolate. The data demonstrated the
susceptibility of T. brasiliensis to DTUs I, II and III, with high rates in simple and mixed
infections reinforcing the importance of T. brasiliensis to the epidemiology of T. cruzi
infection in Rio Grande do Norte. |
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