Nanopartículas lipídicas sólidas de 1,3-diestearil-2-oleil-glicerol funcionalizadas com carga positiva para liberação modificada de proteínas
Solid Lipid Nanoparticles (SLN) are applied colloidal systems as carriers of drugs and biomolecules acting to increase stability and the ability to cross biological barriers. The delivery of proteins as a therapeutic constituent in formulations is a major challenge for the pharmaceutical industry...
Na minha lista:
| Autor principal: | |
|---|---|
| Outros Autores: | |
| Formato: | Dissertação |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
|
| Assuntos: | |
| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/32815 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
| Resumo: | Solid Lipid Nanoparticles (SLN) are applied colloidal systems as carriers of
drugs and biomolecules acting to increase stability and the ability to cross
biological barriers. The delivery of proteins as a therapeutic constituent in
formulations is a major challenge for the pharmaceutical industry in view of the
physico-chemical limitations of these components. In the present study, the
triglyceride 1,3-distearyl-2-oleyl-glycerol (TG1) was chosen as the lipid matrix to
obtain the SLN by emulsification technique with solvent evaporation due to its
potential healing effect. Bovine Serum Albumin (BSA) was chosen as a model
protein because of its similarity to Human Serum Albumin (HSA) and extensive
experimental use. For this, the SLN were functionalized with the positive charge
using the Hyperamed Polyethyleneimine (PEI). SLN were monitored for their size,
polydispersity and zeta potential. The cationic SLN were stable, with sizes below
300 nm and polydispersion in the 0.2 range. The interactions of the protein on the
surface of the particles were accessed by Gel Electrophoresis and Infrared
Spectroscopy (FTIR-ATR). The studies of Atomic Force Microscopy (AFM) pointed
to the spherical shape of the particles. The BSA protein was successfully
incorporated into the cationic NLS (approximately 98%), which had a protracted
release profile. Cell viability assays have shown that SLN decreased PEI toxicity
and BSA loaded cationic SLN further reduced this toxic profile. |
|---|