Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing

The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore,...

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Principais autores: Santos, Yago Queiroz dos, França, Anderson Felipe Jácome de, Veras, Bruno Oliveira de, Carelli, Gabriella Silva Campos, Moura, Geovanna Maria Medeiros, Cruz, Joelton Igor Oliveira da, Oliveira, Fernanda Granja da Silva, Oliveira, João Ricardhis Saturnino de, Amorim, Luciclaudio Cassimiro de, Santos, Elizeu Antunes dos
Formato: article
Idioma:English
Publicado em: Atena Editora
Assuntos:
Endoglucanase
Marine bacteria
rRNA
Endereço do item:https://repositorio.ufrn.br/handle/123456789/32805
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spelling ri-123456789-328052022-04-12T17:29:47Z Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing Santos, Yago Queiroz dos França, Anderson Felipe Jácome de Veras, Bruno Oliveira de Carelli, Gabriella Silva Campos Moura, Geovanna Maria Medeiros Cruz, Joelton Igor Oliveira da Oliveira, Fernanda Granja da Silva Oliveira, João Ricardhis Saturnino de Amorim, Luciclaudio Cassimiro de Santos, Elizeu Antunes dos Endoglucanase Marine bacteria rRNA The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretome 2021-07-05T13:33:48Z 2021-07-05T13:33:48Z 2019 article SANTOS, Yago Queiroz dos et al. Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing. In: SILVA NETO, Benedito Rodrigues da (Org.). Conceitos Básicos da Genética. 1. ed. Ponta Grossa, PR: Atena Editora, 2019. p. 202-209 978-85-7247-421-4 https://repositorio.ufrn.br/handle/123456789/32805 10.22533/at.ed.214192106 en application/pdf Atena Editora
institution Repositório Institucional
collection RI - UFRN
language English
topic Endoglucanase
Marine bacteria
rRNA
spellingShingle Endoglucanase
Marine bacteria
rRNA
Santos, Yago Queiroz dos
França, Anderson Felipe Jácome de
Veras, Bruno Oliveira de
Carelli, Gabriella Silva Campos
Moura, Geovanna Maria Medeiros
Cruz, Joelton Igor Oliveira da
Oliveira, Fernanda Granja da Silva
Oliveira, João Ricardhis Saturnino de
Amorim, Luciclaudio Cassimiro de
Santos, Elizeu Antunes dos
Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
description The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretome
format article
author Santos, Yago Queiroz dos
França, Anderson Felipe Jácome de
Veras, Bruno Oliveira de
Carelli, Gabriella Silva Campos
Moura, Geovanna Maria Medeiros
Cruz, Joelton Igor Oliveira da
Oliveira, Fernanda Granja da Silva
Oliveira, João Ricardhis Saturnino de
Amorim, Luciclaudio Cassimiro de
Santos, Elizeu Antunes dos
author_facet Santos, Yago Queiroz dos
França, Anderson Felipe Jácome de
Veras, Bruno Oliveira de
Carelli, Gabriella Silva Campos
Moura, Geovanna Maria Medeiros
Cruz, Joelton Igor Oliveira da
Oliveira, Fernanda Granja da Silva
Oliveira, João Ricardhis Saturnino de
Amorim, Luciclaudio Cassimiro de
Santos, Elizeu Antunes dos
author_sort Santos, Yago Queiroz dos
title Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
title_short Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
title_full Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
title_fullStr Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
title_full_unstemmed Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
title_sort phylogenetic analysis and identification of a cellulase producing bacillus sp. strain by 16s rrna sequencing
publisher Atena Editora
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/32805
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