Um sistema inteligente de baixo custo para detecção de ácidos nucleicos baseados em eletroforese no espectro visível
With the growing expansion of the Internet of Things, the development of products and services has become more and more automated, a wide range of development opportunities has opened up in several areas. The laboratory environment features various equipment and services that still require compute...
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| Formato: | doctoralThesis |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Rio Grande do Norte
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| Endereço do item: | https://repositorio.ufrn.br/handle/123456789/32782 |
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| Resumo: | With the growing expansion of the Internet of Things, the development of products
and services has become more and more automated, a wide range of development opportunities has opened up in several areas. The laboratory environment features various
equipment and services that still require computerization and development especially focused on low cost, miniaturization and connectivity. Aiming to expand the analysis and
diagnostics to places with low resources. The detection of nucleic acids by electrophoresis is a fast and accessible technique for many diagnostic methods, mainly in research
laboratories or in basic health units. Standard protocols detect nucleic acid molecules
through specific chemical dyes using a UV transilluminator or ultraviolet photodocumentation system. However, the acquisition costs and availability of these devices, especially
those with the ability to photograph and connect to the Internet, are prohibitive, especially
in developing countries. Most nucleic acid detection systems by means of electrophoresis
expose professionals to various risk factors as they use ultraviolet radiation for detection
and also the risk of contact with samples and equipment contaminated by dyes, which
mostly contain mutagenic substances. This work describes the development of a low cost
nucleic acid detection hardware and software system, capable of obtaining qualitative
and semi-quantitative data from gel analysis. The proposed device explores the visible
light absorption range of the commonly used nucleic acid dyes, using easily accessible
electronic devices and fast and simple manufacturing processes. Using spectral tracking
techniques the system covers a wide range of color spectra to detect bands of various
dyes used commercially. The obtaining of the optimum excitation point within the visible
region is possible by detailed analysis of the entire excitation spectrum of the dye and sensitivity in obtaining the wavelengths through the emitting diodes and light. Using the
Internet of things connectivity concepts, we use wireless communication, via Bluetooth,
to develop a human-machine interface on the smartphone to control the proposed device,
capture and share images. The development of the project was designed to allow for scalability of the process, low manufacturing and maintenance costs. The use of LEDs in the
visible spectrum can obtain very reproducible images, providing a high potential for rapid
diagnostics and at the service location, in addition to applications in various fields, such
as health, agriculture and aquaculture. |
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