Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice

Diazepam has been broadly accepted as an anxiolytic drug and is often used as a positive control in behavioral experiments with mice. However, as opposed to this general assumption, the effect of diazepam on mouse behavior can be considered rather controversial from an evidence point of view. Here w...

ver descrição completa

Na minha lista:

Detalhes bibliográficos
Principais autores:	Reis, Marina Pádua, Ferreira, Diana Aline Nôga Morais, Tort, Adriano Bretanha Lopes, Blunder, Martina
Formato:	article
Idioma:	English
Publicado em:	Springer Science and Business Media LLC
Assuntos:	Diazepam - Therapeutic use Hypnotics and sedatives Anti-anxiety agents Mice
Endereço do item:	https://repositorio.ufrn.br/handle/123456789/32780 http://dx.doi.org/10.1038/s41598-021-88599-5
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!

id	ri-123456789-32780
record_format	dspace
spelling	ri-123456789-327802021-06-28T13:45:05Z Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice Reis, Marina Pádua Ferreira, Diana Aline Nôga Morais Tort, Adriano Bretanha Lopes Blunder, Martina Diazepam - Therapeutic use Hypnotics and sedatives Anti-anxiety agents Mice Diazepam has been broadly accepted as an anxiolytic drug and is often used as a positive control in behavioral experiments with mice. However, as opposed to this general assumption, the effect of diazepam on mouse behavior can be considered rather controversial from an evidence point of view. Here we revisit this issue by studying the effect of diazepam on a benchmark task in the preclinical anxiety literature: the elevated plus maze. We evaluated the minute-by-minute time-course of the diazepam effect along the 10 min of the task at three different doses (0.5, 1 and 2 mg/kg i.p. 30 min before the task) in female and male C57BL/6J mice. Furthermore, we contrasted the effects of diazepam with those of a selective serotoninergic reuptake inhibitor (paroxetine, 10 mg/kg i.p. 1 h before the task). Diazepam had no anxiolytic effect at any of the tested doses, and, at the highest dose, it impaired locomotor activity, likely due to sedation. Noteworthy, our results held true when examining male and female mice separately, when only examining the first 5 min of the task, and when animals were subjected to one hour of restrain-induced stress prior to diazepam treatment. In contrast, paroxetine significantly reduced anxiety-like behavior without inducing sedative effects. Our results therefore suggest that preclinical studies for screening new anxiolytic drugs should be cautious with diazepam use as a potential positive control 2021-06-28T13:45:04Z 2021-06-28T13:45:04Z 2021-04-29 article PÁDUA-REIS, Marina; NÔGA, Diana Aline; TORT, Adriano B. L.; BLUNDER, Martina. Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice. Scientific Reports, [S.L.], v. 11, p. 9335, abr. 2021. http://dx.doi.org/10.1038/s41598-021-88599-5. Disponível em: https://www.nature.com/articles/s41598-021-88599-5. Acesso em: 28 jun. 2021. https://repositorio.ufrn.br/handle/123456789/32780 http://dx.doi.org/10.1038/s41598-021-88599-5 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Springer Science and Business Media LLC
institution	Repositório Institucional
collection	RI - UFRN
language	English
topic	Diazepam - Therapeutic use Hypnotics and sedatives Anti-anxiety agents Mice
spellingShingle	Diazepam - Therapeutic use Hypnotics and sedatives Anti-anxiety agents Mice Reis, Marina Pádua Ferreira, Diana Aline Nôga Morais Tort, Adriano Bretanha Lopes Blunder, Martina Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice
description	Diazepam has been broadly accepted as an anxiolytic drug and is often used as a positive control in behavioral experiments with mice. However, as opposed to this general assumption, the effect of diazepam on mouse behavior can be considered rather controversial from an evidence point of view. Here we revisit this issue by studying the effect of diazepam on a benchmark task in the preclinical anxiety literature: the elevated plus maze. We evaluated the minute-by-minute time-course of the diazepam effect along the 10 min of the task at three different doses (0.5, 1 and 2 mg/kg i.p. 30 min before the task) in female and male C57BL/6J mice. Furthermore, we contrasted the effects of diazepam with those of a selective serotoninergic reuptake inhibitor (paroxetine, 10 mg/kg i.p. 1 h before the task). Diazepam had no anxiolytic effect at any of the tested doses, and, at the highest dose, it impaired locomotor activity, likely due to sedation. Noteworthy, our results held true when examining male and female mice separately, when only examining the first 5 min of the task, and when animals were subjected to one hour of restrain-induced stress prior to diazepam treatment. In contrast, paroxetine significantly reduced anxiety-like behavior without inducing sedative effects. Our results therefore suggest that preclinical studies for screening new anxiolytic drugs should be cautious with diazepam use as a potential positive control
format	article
author	Reis, Marina Pádua Ferreira, Diana Aline Nôga Morais Tort, Adriano Bretanha Lopes Blunder, Martina
author_facet	Reis, Marina Pádua Ferreira, Diana Aline Nôga Morais Tort, Adriano Bretanha Lopes Blunder, Martina
author_sort	Reis, Marina Pádua
title	Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice
title_short	Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice
title_full	Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice
title_fullStr	Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice
title_full_unstemmed	Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice
title_sort	diazepam causes sedative rather than anxiolytic effects in c57bl/6j mice
publisher	Springer Science and Business Media LLC
publishDate	2021
url	https://repositorio.ufrn.br/handle/123456789/32780 http://dx.doi.org/10.1038/s41598-021-88599-5
work_keys_str_mv	AT reismarinapadua diazepamcausessedativeratherthananxiolyticeffectsinc57bl6jmice AT ferreiradianaalinenogamorais diazepamcausessedativeratherthananxiolyticeffectsinc57bl6jmice AT tortadrianobretanhalopes diazepamcausessedativeratherthananxiolyticeffectsinc57bl6jmice AT blundermartina diazepamcausessedativeratherthananxiolyticeffectsinc57bl6jmice
_version_	1773961530179584000

Diazepam causes sedative rather than anxiolytic effects in C57BL/6J mice

Registros relacionados