Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing

This study evaluated the hydrothermal, alkaline (NaOH) and acid (H2SO4) pretreatment all combined with Tween 80 of the green coconut fiber aiming to improve the fermentable sugars production during the enzymatic hydrolysis. In addition, the water use during the post-washing of the pretreated mater...

詳細記述

保存先:
書誌詳細
主要な著者: Santos, Everaldo Silvino dos, Nogueira, Cleitiane da Costa, Padilha, Carlos Eduardo de Araújo, Leitão, Ana Laura de Sá, Rocha, Patrícia Maria, Macedo, Gorete Ribeiro de
フォーマット: article
言語:English
出版事項: Elsevier
主題:
Acid
Alkaline
Hydrothermal
Surfactant
Washing
Coconut fiber
オンライン･アクセス:https://repositorio.ufrn.br/handle/123456789/32628
タグ: タグ追加
タグなし, このレコードへの初めてのタグを付けませんか!
id ri-123456789-32628
record_format dspace
spelling ri-123456789-326282023-02-07T19:25:46Z Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing Santos, Everaldo Silvino dos Nogueira, Cleitiane da Costa Padilha, Carlos Eduardo de Araújo Leitão, Ana Laura de Sá Rocha, Patrícia Maria Macedo, Gorete Ribeiro de Acid Alkaline Hydrothermal Surfactant Washing Coconut fiber This study evaluated the hydrothermal, alkaline (NaOH) and acid (H2SO4) pretreatment all combined with Tween 80 of the green coconut fiber aiming to improve the fermentable sugars production during the enzymatic hydrolysis. In addition, the water use during the post-washing of the pretreated materials was also assayed, seeking to ration its use without compromising the enzymatic hydrolysis and the removal of inhibitors. Untreated and pretreated biomass were assayed in terms of total solids, extractables, polysaccharides, lignin and total ashes according to the protocols proposed by NREL. Enzymatic hydrolysis was carried out using the preparation of cellulases from Trichoderma reesei ATCC 26921 and β-glucosidases (NS-22118 DCN00218) and xylanases (NS-22036 CDN01015). Additionally, the mass loss during pretreatment and washing was estimated. Results showed that the use of 3.0% (w/w) Tween 80 in the dilute alkaline pretreatment (2.0% (w/v) NaOH, 121 °C, 10 min) increased the conversion into glucose during the enzymatic hydrolysis of the pretreated materials from 48.7% to 56.1%. However, it did not show any influence on the pretreated materials in acid and hydrothermal conditions. The evaluation of the washing of the pretreated materials reduced the volume of water initially used in up to 75%, without affecting the final sugar production and the reduction of inhibitory compounds in the residual water, contributing to the viability of the production of cellulosic ethanol from green coconut fiber 2021-06-10T13:59:40Z 2021-06-10T13:59:40Z 2018-02 article NOGUEIRA, C. C.; PADILHA, C. E.; LEITAO, A. L. S.; ROCHA, P. M.; MACEDO, G. R.; SANTOS, E. S.. Enhancing enzymatic hydrolysis of green coconut fiber-Pretreatment assisted by tween 80 and water effect on the post-washing. Industrial Crops and Products, v. 112, p. 734-740, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0926669017308804?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.indcrop.2017.12.047. 0926-6690 https://repositorio.ufrn.br/handle/123456789/32628 10.1016/j.indcrop.2017.12.047 en Elsevier
institution Repositório Institucional
collection RI - UFRN
language English
topic Acid
Alkaline
Hydrothermal
Surfactant
Washing
Coconut fiber
spellingShingle Acid
Alkaline
Hydrothermal
Surfactant
Washing
Coconut fiber
Santos, Everaldo Silvino dos
Nogueira, Cleitiane da Costa
Padilha, Carlos Eduardo de Araújo
Leitão, Ana Laura de Sá
Rocha, Patrícia Maria
Macedo, Gorete Ribeiro de
Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
description This study evaluated the hydrothermal, alkaline (NaOH) and acid (H2SO4) pretreatment all combined with Tween 80 of the green coconut fiber aiming to improve the fermentable sugars production during the enzymatic hydrolysis. In addition, the water use during the post-washing of the pretreated materials was also assayed, seeking to ration its use without compromising the enzymatic hydrolysis and the removal of inhibitors. Untreated and pretreated biomass were assayed in terms of total solids, extractables, polysaccharides, lignin and total ashes according to the protocols proposed by NREL. Enzymatic hydrolysis was carried out using the preparation of cellulases from Trichoderma reesei ATCC 26921 and β-glucosidases (NS-22118 DCN00218) and xylanases (NS-22036 CDN01015). Additionally, the mass loss during pretreatment and washing was estimated. Results showed that the use of 3.0% (w/w) Tween 80 in the dilute alkaline pretreatment (2.0% (w/v) NaOH, 121 °C, 10 min) increased the conversion into glucose during the enzymatic hydrolysis of the pretreated materials from 48.7% to 56.1%. However, it did not show any influence on the pretreated materials in acid and hydrothermal conditions. The evaluation of the washing of the pretreated materials reduced the volume of water initially used in up to 75%, without affecting the final sugar production and the reduction of inhibitory compounds in the residual water, contributing to the viability of the production of cellulosic ethanol from green coconut fiber
format article
author Santos, Everaldo Silvino dos
Nogueira, Cleitiane da Costa
Padilha, Carlos Eduardo de Araújo
Leitão, Ana Laura de Sá
Rocha, Patrícia Maria
Macedo, Gorete Ribeiro de
author_facet Santos, Everaldo Silvino dos
Nogueira, Cleitiane da Costa
Padilha, Carlos Eduardo de Araújo
Leitão, Ana Laura de Sá
Rocha, Patrícia Maria
Macedo, Gorete Ribeiro de
author_sort Santos, Everaldo Silvino dos
title Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
title_short Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
title_full Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
title_fullStr Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
title_full_unstemmed Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing
title_sort enhancing enzymatic hydrolysis of green coconut fiber—pretreatment assisted by tween 80 and water effect on the post-washing
publisher Elsevier
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/32628
work_keys_str_mv AT santoseveraldosilvinodos enhancingenzymatichydrolysisofgreencoconutfiberpretreatmentassistedbytween80andwatereffectonthepostwashing
AT nogueiracleitianedacosta enhancingenzymatichydrolysisofgreencoconutfiberpretreatmentassistedbytween80andwatereffectonthepostwashing
AT padilhacarloseduardodearaujo enhancingenzymatichydrolysisofgreencoconutfiberpretreatmentassistedbytween80andwatereffectonthepostwashing
AT leitaoanalauradesa enhancingenzymatichydrolysisofgreencoconutfiberpretreatmentassistedbytween80andwatereffectonthepostwashing
AT rochapatriciamaria enhancingenzymatichydrolysisofgreencoconutfiberpretreatmentassistedbytween80andwatereffectonthepostwashing
AT macedogoreteribeirode enhancingenzymatichydrolysisofgreencoconutfiberpretreatmentassistedbytween80andwatereffectonthepostwashing
_version_ 1773965440046858240

類似資料