Chitooligosaccharides antagonize the cytotoxic effect of glucosamine

Chitooligosaccharides (COS) are partially hydrolyzed compounds derived from chitosan that exhibit a number of biological activities, including antitumor, antibacterial and antifungal properties. In this work, we examined the cytotoxicity of pure COS and oligomers A, B and C (solutions composed of di...

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Principais autores: Santos, Everaldo Silvino dos, Assis, Cristiane Fernandes de, Costa, Leandro Silva, Melo-Silveira, Raniere Fagundes, Oliveira, Ruth Medeiros, Pagnoncelli, Maria Giovana Binder, Rocha, Hugo Alexandre Oliveira, Macedo, Gorete Ribeiro de
Formato: article
Idioma:English
Publicado em: Springer
Assuntos:
Glucosamine
Chitosan
Metarhrizium anisopliae
HeLa
HepG2
3T3
Endereço do item:https://repositorio.ufrn.br/handle/123456789/32557
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spelling ri-123456789-325572021-05-25T13:18:25Z Chitooligosaccharides antagonize the cytotoxic effect of glucosamine Santos, Everaldo Silvino dos Assis, Cristiane Fernandes de Costa, Leandro Silva Melo-Silveira, Raniere Fagundes Oliveira, Ruth Medeiros Pagnoncelli, Maria Giovana Binder Rocha, Hugo Alexandre Oliveira Macedo, Gorete Ribeiro de Glucosamine Chitosan Metarhrizium anisopliae HeLa HepG2 3T3 Chitooligosaccharides (COS) are partially hydrolyzed compounds derived from chitosan that exhibit a number of biological activities, including antitumor, antibacterial and antifungal properties. In this work, we examined the cytotoxicity of pure COS and oligomers A, B and C (solutions composed of different amounts of COS) produced by enzymatic hydrolysis using a crude enzyme extract produced by the fungus Metarhrizium anisopliae. The antiproliferative effect of these molecules was analyzed using tumor cell lines (HepG2 and HeLa cells) and in a normal cell line (3T3). The antioxidant activity was analyzed in several in vitro experiments. Glucosamine showed higher toxicity (approximately 92%) to all cell lines studied. However, the oligomers obtained after hydrolysis demonstrated no toxic effects on the normal cells (3T3). Furthermore, we showed that a small amount of other COS can decrease the cytotoxic effect of glucosamine against 3T3 cells, indicating that glucosamine could be used as an antitumor drug in the presence of other COS. In addition, different effects were found in antiproliferative assays, which depended on the COS composition in the oligomers (A, B and C), showing that a combination of them may be essential for developing antineoplastic drugs. Superoxide anion scavenging was the main antioxidant activity demonstrated by the COS and oligomers. This activity was also dependent on the oligomer composition of the chitosan hydrolysates. Further work will identify the ideal proportions of COS and glucosamine for maximizing the effects of these biological activities 2021-05-25T13:18:23Z 2021-05-25T13:18:23Z 2012-10-02 article ASSIS, Cristiane Fernandes de; COSTA, Leandro Silva; MELO-SILVEIRA, Raniere Fagundes; OLIVEIRA, Ruth Medeiros; PAGNONCELLI, Maria Giovana Binder; ROCHA, Hugo Alexandre Oliveira; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Chitooligosaccharides antagonize the cytotoxic effect of glucosamine. World Journal Of Microbiology And Biotechnology, [S.L.], v. 28, n. 3, p. 1097-1105, 2 out. 2011. Disponível em: https://link.springer.com/article/10.1007%2Fs11274-011-0910-4. Acesso em: 08 abr. 2021. http://dx.doi.org/10.1007/s11274-011-0910-4. 0959-3993 1573-0972 https://repositorio.ufrn.br/handle/123456789/32557 10.1007/s11274-011-0910-4 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Springer
institution Repositório Institucional
collection RI - UFRN
language English
topic Glucosamine
Chitosan
Metarhrizium anisopliae
HeLa
HepG2
3T3
spellingShingle Glucosamine
Chitosan
Metarhrizium anisopliae
HeLa
HepG2
3T3
Santos, Everaldo Silvino dos
Assis, Cristiane Fernandes de
Costa, Leandro Silva
Melo-Silveira, Raniere Fagundes
Oliveira, Ruth Medeiros
Pagnoncelli, Maria Giovana Binder
Rocha, Hugo Alexandre Oliveira
Macedo, Gorete Ribeiro de
Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
description Chitooligosaccharides (COS) are partially hydrolyzed compounds derived from chitosan that exhibit a number of biological activities, including antitumor, antibacterial and antifungal properties. In this work, we examined the cytotoxicity of pure COS and oligomers A, B and C (solutions composed of different amounts of COS) produced by enzymatic hydrolysis using a crude enzyme extract produced by the fungus Metarhrizium anisopliae. The antiproliferative effect of these molecules was analyzed using tumor cell lines (HepG2 and HeLa cells) and in a normal cell line (3T3). The antioxidant activity was analyzed in several in vitro experiments. Glucosamine showed higher toxicity (approximately 92%) to all cell lines studied. However, the oligomers obtained after hydrolysis demonstrated no toxic effects on the normal cells (3T3). Furthermore, we showed that a small amount of other COS can decrease the cytotoxic effect of glucosamine against 3T3 cells, indicating that glucosamine could be used as an antitumor drug in the presence of other COS. In addition, different effects were found in antiproliferative assays, which depended on the COS composition in the oligomers (A, B and C), showing that a combination of them may be essential for developing antineoplastic drugs. Superoxide anion scavenging was the main antioxidant activity demonstrated by the COS and oligomers. This activity was also dependent on the oligomer composition of the chitosan hydrolysates. Further work will identify the ideal proportions of COS and glucosamine for maximizing the effects of these biological activities
format article
author Santos, Everaldo Silvino dos
Assis, Cristiane Fernandes de
Costa, Leandro Silva
Melo-Silveira, Raniere Fagundes
Oliveira, Ruth Medeiros
Pagnoncelli, Maria Giovana Binder
Rocha, Hugo Alexandre Oliveira
Macedo, Gorete Ribeiro de
author_facet Santos, Everaldo Silvino dos
Assis, Cristiane Fernandes de
Costa, Leandro Silva
Melo-Silveira, Raniere Fagundes
Oliveira, Ruth Medeiros
Pagnoncelli, Maria Giovana Binder
Rocha, Hugo Alexandre Oliveira
Macedo, Gorete Ribeiro de
author_sort Santos, Everaldo Silvino dos
title Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
title_short Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
title_full Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
title_fullStr Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
title_full_unstemmed Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
title_sort chitooligosaccharides antagonize the cytotoxic effect of glucosamine
publisher Springer
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/32557
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