Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
The branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellu...
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ri-123456789-325492021-05-21T19:22:13Z Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis Soares, Deivid Costa Courtial, Camila Gambini Pereira Meireles, Maria Angela de Almeida Saraiva, Elvira Maria Leishmanicidal activity Tabernaemontana Leishmania Supercritical Fluid Extraction The branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellular amastigotes of Leishmania amazonensis, a causative agent of New World cutaneous leishmaniasis. AF3 inhibits Leishmania survival in a dose-dependent manner, and reached 88% inhibition of amastigote growth at 100 μg/mL. The anti-parasite effect was independent of nitric oxide (NO), since AF3 was able to inhibit NO production induced by IFN-γ plus LPS. In addition, AF3 inhibited TGF-β production, which could have facilitated AF3-mediated parasite killing. The AF3 fraction obtained from SFE was nontoxic for host macrophages, as assessed by plasma membrane integrity and mitochondrial activity. We conclude that SFE is an efficient method for obtaining bioactive indole alkaloids from plant extracts. Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cells 2021-05-21T19:22:12Z 2021-05-21T19:22:12Z 2007-06 article SOARES, D. C.; PEREIRA, C. G.; MEIRELES, M.A.A.; SARAIVA, E. M.. Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis. Parasitology International, v. 56, p. 135-139, 2007. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1383576907000074?via%3Dihub. Acesso em: 08 abr. 2021. https://doi.org/10.1016/j.parint.2007.01.004. 1383-5769 https://repositorio.ufrn.br/handle/123456789/32549 10.1016/j.parint.2007.01.004 en Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ application/pdf Elsevier |
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Leishmanicidal activity Tabernaemontana Leishmania Supercritical Fluid Extraction |
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Leishmanicidal activity Tabernaemontana Leishmania Supercritical Fluid Extraction Soares, Deivid Costa Courtial, Camila Gambini Pereira Meireles, Maria Angela de Almeida Saraiva, Elvira Maria Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis |
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The branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellular amastigotes of Leishmania amazonensis, a causative agent of New World cutaneous leishmaniasis. AF3 inhibits Leishmania
survival in a dose-dependent manner, and reached 88% inhibition of amastigote growth at 100 μg/mL. The anti-parasite effect was independent of nitric oxide (NO), since AF3 was able to inhibit NO production induced by IFN-γ plus LPS. In addition, AF3 inhibited TGF-β production, which could have facilitated AF3-mediated parasite killing. The AF3 fraction obtained from SFE was nontoxic for host macrophages, as assessed by
plasma membrane integrity and mitochondrial activity. We conclude that SFE is an efficient method for obtaining bioactive indole alkaloids from plant extracts. Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cells |
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article |
author |
Soares, Deivid Costa Courtial, Camila Gambini Pereira Meireles, Maria Angela de Almeida Saraiva, Elvira Maria |
author_facet |
Soares, Deivid Costa Courtial, Camila Gambini Pereira Meireles, Maria Angela de Almeida Saraiva, Elvira Maria |
author_sort |
Soares, Deivid Costa |
title |
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis |
title_short |
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis |
title_full |
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis |
title_fullStr |
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis |
title_full_unstemmed |
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis |
title_sort |
leishmanicidal activity of a supercritical fluid fraction obtained from tabernaemontana catharinensis |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://repositorio.ufrn.br/handle/123456789/32549 |
work_keys_str_mv |
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_version_ |
1773959400013168640 |