Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography

Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Some authors have reported that as much as 50% of overall costs in...

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Principais autores: Santos, Everaldo Silvino dos, Sousa Junior, Francisco Canindé de, Vaz, Michelle Rossana Ferreira, Padilha, Carlos Eduardo de Araújo, Martins, Daniella Regina Arantes, Macedo, Gorete Ribeiro de
Formato: article
Idioma:English
Publicado em: BMC
Assuntos:
Visceral Leishmaniasis
Leishmaniasis
Elution Condition
Batch Adsorption Experiment
Leishmania Infantum
Endereço do item:https://repositorio.ufrn.br/handle/123456789/32543
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spelling ri-123456789-325432021-05-21T01:52:32Z Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography Santos, Everaldo Silvino dos Sousa Junior, Francisco Canindé de Vaz, Michelle Rossana Ferreira Padilha, Carlos Eduardo de Araújo Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro de Visceral Leishmaniasis Leishmaniasis Elution Condition Batch Adsorption Experiment Leishmania Infantum Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Some authors have reported that as much as 50% of overall costs in the biotechnology industries are related to downstream processing. Thus, the development of new and economically advantageous purification methods is a challenge [2]. Expanded bed adsorption (EBA) is an innovative chromatography technology that allows the adsorption of target proteins directly from unclarified feedstock. EBA technology combines solid-liquid separation with an adsorption step in a single-unit operation, aiming at increased overall yield, reduced operational time, and less capital investment and consumables [3,4]. Thus, the aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from crude feedstock using EBA chromatography 2021-05-21T01:52:31Z 2021-05-21T01:52:31Z 2014-10-01 article SOUSA JUNIOR, Francisco Canindé de; VAZ, Michelle Rossana Ferreira; PADILHA, Carlos Eduardo de Araújo; MARTINS, Daniella Regina Arantes; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography. Bmc Proceedings, [S.L.], v. 8, n. 4, out. 2014. Disponível em: https://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-8-S4-P194. Acesso em: 08 abr. 2021. https://doi.org/10.1186/1753-6561-8-S4-P194 1753-6561 https://repositorio.ufrn.br/handle/123456789/32543 10.1186/1753-6561-8-S4-P194 en Attribution 3.0 Brazil Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ http://creativecommons.org/licenses/by/3.0/br/ application/pdf BMC
institution Repositório Institucional
collection RI - UFRN
language English
topic Visceral Leishmaniasis
Leishmaniasis
Elution Condition
Batch Adsorption Experiment
Leishmania Infantum
spellingShingle Visceral Leishmaniasis
Leishmaniasis
Elution Condition
Batch Adsorption Experiment
Leishmania Infantum
Santos, Everaldo Silvino dos
Sousa Junior, Francisco Canindé de
Vaz, Michelle Rossana Ferreira
Padilha, Carlos Eduardo de Araújo
Martins, Daniella Regina Arantes
Macedo, Gorete Ribeiro de
Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
description Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Some authors have reported that as much as 50% of overall costs in the biotechnology industries are related to downstream processing. Thus, the development of new and economically advantageous purification methods is a challenge [2]. Expanded bed adsorption (EBA) is an innovative chromatography technology that allows the adsorption of target proteins directly from unclarified feedstock. EBA technology combines solid-liquid separation with an adsorption step in a single-unit operation, aiming at increased overall yield, reduced operational time, and less capital investment and consumables [3,4]. Thus, the aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from crude feedstock using EBA chromatography
format article
author Santos, Everaldo Silvino dos
Sousa Junior, Francisco Canindé de
Vaz, Michelle Rossana Ferreira
Padilha, Carlos Eduardo de Araújo
Martins, Daniella Regina Arantes
Macedo, Gorete Ribeiro de
author_facet Santos, Everaldo Silvino dos
Sousa Junior, Francisco Canindé de
Vaz, Michelle Rossana Ferreira
Padilha, Carlos Eduardo de Araújo
Martins, Daniella Regina Arantes
Macedo, Gorete Ribeiro de
author_sort Santos, Everaldo Silvino dos
title Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
title_short Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
title_full Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
title_fullStr Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
title_full_unstemmed Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
title_sort production, recovery, and purification of recombinant 503 antigen of leishmania infantum chagasi using expanded bed adsorption chromatography
publisher BMC
publishDate 2021
url https://repositorio.ufrn.br/handle/123456789/32543
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